Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C7T95_RS01950 Genome accession   NZ_CP028730
Coordinates   474099..475811 (-) Length   570 a.a.
NCBI ID   WP_107946384.1    Uniprot ID   -
Organism   Shewanella baltica strain 128     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 469099..480811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7T95_RS01930 coaE 469140..469757 (-) 618 WP_063884698.1 dephospho-CoA kinase -
  C7T95_RS01935 - 469759..471714 (-) 1956 WP_107946381.1 LTA synthase family protein -
  C7T95_RS01940 pilD 471731..472660 (-) 930 WP_107946382.1 prepilin peptidase Machinery gene
  C7T95_RS01945 pilC 472693..473961 (-) 1269 WP_107946383.1 type II secretion system F family protein Machinery gene
  C7T95_RS01950 pilB 474099..475811 (-) 1713 WP_107946384.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C7T95_RS01955 - 475883..476308 (-) 426 WP_107946385.1 type IV pilin protein -
  C7T95_RS01960 nadC 477017..477898 (-) 882 WP_107946386.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C7T95_RS01965 ampD 478170..478733 (+) 564 WP_107946387.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C7T95_RS01970 ampE 478803..479654 (+) 852 WP_006083346.1 beta-lactamase regulator AmpE -
  C7T95_RS01975 pdhR 479893..480645 (+) 753 WP_006083345.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62915.40 Da        Isoelectric Point: 6.2567

>NTDB_id=286816 C7T95_RS01950 WP_107946384.1 474099..475811(-) (pilB) [Shewanella baltica strain 128]
MPTTGLHLGLSTLFIRKGLLNEEQMANAITKSRQNKHALVSTLVQSKLISARAVAELCYEEYGTPLLDLNEFDLASIPED
FLNKKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEEDKLAKALEKILEEDISGLDLGGMDEAA
LAAIEITDTDRRNEEPTGESSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQAEIIPEHELLRLGF
SQTQITQGFTTFKPIGCEHCSAGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKAQGMRDLRQSGLLKVIHGVTSI
AEVNRVTSFG

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=286816 C7T95_RS01950 WP_107946384.1 474099..475811(-) (pilB) [Shewanella baltica strain 128]
ATGCCCACTACTGGATTGCATTTAGGATTATCGACTCTATTCATACGTAAAGGTCTGCTAAATGAAGAGCAGATGGCTAA
CGCTATCACTAAATCTCGACAAAACAAACACGCCCTTGTAAGCACCTTAGTTCAAAGTAAGCTAATCTCTGCCCGTGCAG
TAGCCGAGCTTTGCTATGAAGAGTACGGAACACCACTACTCGATCTCAATGAATTTGATCTTGCTTCCATTCCTGAGGAT
TTTCTCAATAAAAAGCTGATTGAGAAACACCGCTGCTTGCCCTTATTTAAACGTGGGAATCGCCTCTATATAGGCACATC
CGACCCAACCAATATTGCCGCCCTAGAAGATTTCCAATTTAGTGCAGGCCTGCATGCCGAAGCGATTCTAGTTGAGGAAG
ATAAGCTCGCTAAAGCGCTAGAGAAAATTCTTGAAGAAGATATCAGCGGTCTCGATTTAGGCGGTATGGACGAAGCAGCG
CTGGCAGCGATAGAAATTACCGACACAGATAGACGTAATGAAGAGCCTACTGGCGAGAGTAGTGACGATGCACCCATCGT
TATTTATATCAATAAAATTTTAACCGATGCGATTCGCAAAGGCGCCTCGGATTTGCACTTTGAACCCTATGAGAAACGCT
ACCGTATTCGTTTTCGTATAGATGGTATTCTCCACGAAGTCTCTGAACCGCCGATTAGTCTGGCTGGTCGTCTTTCTGCA
CGCTTAAAAGTGATGTCGAAACTCGATATCGCCGAACGACGCGTGCCACAGGATGGTCGAATCAAGATGAAACTGTCCCG
CACTAAGTCTATCGACTTTCGGGTTAGTACTCTGCCGACCCTGTGGGGTGAAAAGATTGTAATGCGGATTTTAGACTCAT
CCTCCGCACAATTAGGCATTGAAAAGCTCGGCTACGAGCCAGACCAAGAAAAGCTCTACCTTGAGATGCTAGCAAAACCC
CAAGGGATGATTTTAGTAACGGGGCCCACAGGTTCTGGTAAAACCGTCTCCCTCTACACTGGGTTAAATATTCTCAATAC
CGAAGAGCGTAATATATCTACCGCAGAAGATCCGGTTGAGATTAACCTTGAAGGCGTGAACCAAGTTCACATTAATTTAA
AAGCTGGTCTCACTTTTGCCTCAGCTTTACGCTCATTCCTGCGTCAAGACCCCGATGTGGTGATGGTAGGAGAAATCCGT
GATCTCGAAACCGCCGAAATTGCCATTAAAGCGGCGCAAACGGGTCACTTAGTCTTATCAACCTTGCACACAAACTCGGC
TGCAGAAACCTTAACTCGTTTAATCAACATGGGCGTGCCTGGTTATAATATCGCCAGTTCTGTGAATCTGATTATTGCCC
AGCGTTTGGCCCGTCGACTTTGCCCCGAATGTAAACAAGCGGAAATAATCCCCGAGCATGAACTATTACGTTTAGGTTTT
TCTCAAACACAAATTACTCAGGGCTTTACTACCTTTAAACCCATTGGCTGCGAACATTGCTCTGCAGGCTACAAGGGACG
GGTTGGCATTTATGAAGTGATGAAGATGTCTGACGAAATAGCCCGTACTATCATGGAGGGCGGCAACTCTTTGCAAATCG
CCTCCCAAGCTAAAGCCCAAGGAATGCGAGATTTACGACAATCCGGATTATTGAAAGTGATTCACGGTGTCACTAGCATT
GCAGAAGTCAATCGTGTCACCAGTTTTGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.18

98.421

0.582
  pilB Acinetobacter baumannii D1279779

58.899

95.614

0.563
  pilB Legionella pneumophila strain ERS1305867

55.694

98.596

0.549
  pilB Vibrio cholerae strain A1552

53.153

97.368

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.761

99.649

0.516
  pilB Vibrio campbellii strain DS40M4

51.504

99.123

0.511
  pilF Neisseria gonorrhoeae MS11

47.527

99.298

0.472
  pilF Thermus thermophilus HB27

39.046

99.298

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.71

97.895

0.379

Multiple sequence alignment