Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSPA7_RS27565 Genome accession   NC_009656
Coordinates   5968124..5970268 (-) Length   714 a.a.
NCBI ID   WP_012077720.1    Uniprot ID   A6VDG1
Organism   Pseudomonas paraeruginosa PA7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5963124..5975268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSPA7_RS27550 (PSPA7_5774) - 5964785..5966431 (-) 1647 WP_012077718.1 AAA family ATPase -
  PSPA7_RS27555 (PSPA7_5775) aroB 5966438..5967544 (-) 1107 WP_012077719.1 3-dehydroquinate synthase -
  PSPA7_RS27560 (PSPA7_5776) aroK 5967594..5968112 (-) 519 WP_003148769.1 shikimate kinase AroK -
  PSPA7_RS27565 (PSPA7_5777) pilQ 5968124..5970268 (-) 2145 WP_012077720.1 type 4a pilus secretin PilQ Machinery gene
  PSPA7_RS27570 (PSPA7_5778) pilP 5970322..5970843 (-) 522 WP_012077721.1 type 4a pilus biogenesis lipoprotein PilP -
  PSPA7_RS27575 (PSPA7_5779) pilO 5970840..5971463 (-) 624 WP_003148775.1 type 4a pilus biogenesis protein PilO -
  PSPA7_RS27580 (PSPA7_5780) pilN 5971460..5972056 (-) 597 WP_003148777.1 type 4a pilus biogenesis protein PilN -
  PSPA7_RS27585 (PSPA7_5781) pilM 5972056..5973120 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77396.06 Da        Isoelectric Point: 5.3173

>NTDB_id=28559 PSPA7_RS27565 WP_012077720.1 5968124..5970268(-) (pilQ) [Pseudomonas paraeruginosa PA7]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDQPVATPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASAAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFINASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTADDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SEGGAEGKEGGRGSITVDDRTNSIIAYQPQDRLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
NAYGKDGNMGIKDEEGLNCGPIAGNCSFPTVGTNKSPSPFVDIGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIIS
QPKVVTSDKETAKILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDYQNMLNGVPPINKN
EVNAKILVNDGETIVIGGVFSNEQTKAVDKVPFLGDLPYLGRLFRRDTVVDRKNELLVFLAPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=28559 PSPA7_RS27565 WP_012077720.1 5968124..5970268(-) (pilQ) [Pseudomonas paraeruginosa PA7]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCCTTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCGCTGCCGGGAGACCGGGTCGAGCTCAAGCTGCAGTTCGACCAGCCCGTCGCCACGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGGATCGCCCTCGACCTGCCGGGGGTGCAGAACAAGCTCGGCACCAAGAACCGCGAA
CTGAGCGTGGGCAATACACGCAGCGTCACGGTGGTCGAGGCGAAGGACCGCACACGGTTGATCATCAACCTGACCACGCT
GTCGTCCTACACCACCCGGGTGGAGGGCAACAACCTGTTCGTAGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCGCCGCTCCGGTCAAGGCGAGCCCGGCTCCGGCCAGCTACGCCCAGCCGATCAAGCCCAAGCCGTATGTGCCGGCGGGC
CGCGCGATCCGCAATATCGACTTCCAACGCGGCGAGAAGGGCGAGGGCAACGTGGTCATCGACCTCTCCGACCCGACGCT
GAGCCCGGATATCCAGGAACAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGCTGCCCGACGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACCCCGGTGCAGTTCATCAACGCCAGTGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTACCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCGCGGA
CGACGCCGAGCGGCGCAAGAAGGACAACTTCGCCTACACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGC
GGTCGGTGCTGCAATTGATCGCCGACTTCACCGATCTGAACCTGGTGGCCTCGGATACCGTGCAGGGCAACATCACCTTG
CGCCTGCAGAATGTGCCGTGGGACCAGGCGCTGGACCTCGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTGCTGGTGGCACCGGCCGACGAGATCGCCGCACGCGAGCGTCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAAC
TGGCGCCGCTGCGTCGCGAGCTGATCCAGGTGAACTACGCCAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGAGGGCGGTGCGGAAGGCAAGGAAGGCGGGCGCGGCTCGATCACAGTGGACGACCGCACCAACAGCATCATCGCCTA
CCAGCCGCAAGATCGCCTGGATGAGCTGCGGCGGATCGTTTCGCAACTGGACATCCCGGTACGCCAGGTGATGATCGAGG
CGCGCATCGTCGAAGCCAACGTCGGCTACGACAAGAGCCTCGGCGTGCGCTGGGGCGGTGCCTACCACAAGGGCAACTGG
AATGCCTACGGCAAGGACGGCAACATGGGGATCAAGGACGAGGAAGGCCTGAACTGCGGTCCCATCGCCGGCAACTGTAG
CTTCCCCACCGTGGGCACCAACAAGTCGCCGTCGCCGTTCGTCGATATTGGCGCGAAGGATGCCACTTCCGGGATCGGCA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAATTGTCGGCGATGGAAAAGACCGGCAACGGTGAAATCATTTCT
CAGCCGAAGGTGGTCACTTCCGACAAGGAAACCGCGAAGATCCTCAAAGGCTCCGAGGTTCCCTATCAGGAGGCCAGCTC
CAGCGGCGCCACCAGTACCTCCTTCAAGGAGGCTGCGCTGTCCCTCGAAGTGACCCCGCAGATCACGCCGGACAACCGGA
TCATCGTCGAGGTCAAGGTCACCAAGGATGCGCCGGACTACCAGAACATGCTGAATGGCGTACCGCCGATCAACAAGAAC
GAGGTCAACGCCAAGATCCTGGTCAACGATGGCGAAACCATCGTCATCGGCGGGGTGTTCAGCAACGAGCAGACCAAGGC
GGTGGACAAGGTGCCGTTCCTCGGCGACCTGCCCTACCTGGGCCGCCTGTTCCGGCGTGACACGGTGGTCGACAGGAAAA
ACGAACTTCTGGTTTTCCTGGCACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(307-353)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(549-705)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A6VDG1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

96.359

100

0.964
  comQ Acinetobacter baylyi ADP1

40.782

100

0.409
  pilQ Acinetobacter baumannii strain A118

40.278

100

0.406
  pilQ Acinetobacter baumannii D1279779

40.278

100

0.406
  pilQ Legionella pneumophila strain Lp02

37.662

97.059

0.366
  pilQ Legionella pneumophila strain ERS1305867

37.662

97.059

0.366

Multiple sequence alignment