Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PSPA7_RS27585 Genome accession   NC_009656
Coordinates   5972056..5973120 (-) Length   354 a.a.
NCBI ID   WP_003110888.1    Uniprot ID   -
Organism   Pseudomonas paraeruginosa PA7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5967056..5978120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSPA7_RS27560 (PSPA7_5776) aroK 5967594..5968112 (-) 519 WP_003148769.1 shikimate kinase AroK -
  PSPA7_RS27565 (PSPA7_5777) pilQ 5968124..5970268 (-) 2145 WP_012077720.1 type 4a pilus secretin PilQ Machinery gene
  PSPA7_RS27570 (PSPA7_5778) pilP 5970322..5970843 (-) 522 WP_012077721.1 type 4a pilus biogenesis lipoprotein PilP -
  PSPA7_RS27575 (PSPA7_5779) pilO 5970840..5971463 (-) 624 WP_003148775.1 type 4a pilus biogenesis protein PilO -
  PSPA7_RS27580 (PSPA7_5780) pilN 5971460..5972056 (-) 597 WP_003148777.1 type 4a pilus biogenesis protein PilN -
  PSPA7_RS27585 (PSPA7_5781) pilM 5972056..5973120 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PSPA7_RS27590 (PSPA7_5782) - 5973305..5975776 (+) 2472 WP_012077723.1 penicillin-binding protein 1A -
  PSPA7_RS27595 (PSPA7_5783) - 5975881..5977149 (-) 1269 WP_012077724.1 malic enzyme -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37983.48 Da        Isoelectric Point: 4.5405

>NTDB_id=28560 PSPA7_RS27585 WP_003110888.1 5972056..5973120(-) (pilM) [Pseudomonas paraeruginosa PA7]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=28560 PSPA7_RS27585 WP_003110888.1 5972056..5973120(-) (pilM) [Pseudomonas paraeruginosa PA7]
GTGCTAGGGCTCATAAAAAAGAAAGCGAATACCTTACTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAACGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAATCGGCCGTGGTG
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACGATCGAGATGGAGGCTGGGCTTTCCGAGGACGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAGGTCGCCATCGACTTCGAGGTCCAGGGTCTTTCCGCGC
GCAATCCCGAGCGCGTGGACGTGCTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTGCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGTTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTGGTGGACATCGGCGCGACCATGACCACGCTCAGCGTCCTGCACAACGGCC
GCACCATCTATACCCGCGAGCAGTTGTTCGGCGGACGCCAGCTCACCGAGGAGATCCAGCGGCGCTACGGGCTTTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGTGGTCTTCCGGATGACTACGACAGCGAAGTCCTGCGTCCGTTCAAGGACGC
CGTGGTCCAGCAGGTTTCCCGTTCGCTGCAGTTCTTCTTCGCCGCCGGCCAGTTCAATGACGTCGACTACATCGTCCTGG
CGGGCGGCACGGCGTCGATCCAGGACCTCGACCGGCTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAACGCCGGTGCCCTGGCCAGCGACGCGCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.899

100

0.562
  comM Acinetobacter nosocomialis M2

55.899

100

0.562
  comM Acinetobacter baylyi ADP1

51.977

100

0.52
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466

Multiple sequence alignment