Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ASUC_RS03185 Genome accession   NC_009655
Coordinates   675227..676669 (-) Length   480 a.a.
NCBI ID   WP_012072366.1    Uniprot ID   A6VLY9
Organism   Actinobacillus succinogenes 130Z     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 670227..681669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASUC_RS03155 (Asuc_0606) nqrM 670622..670867 (+) 246 WP_012072360.1 (Na+)-NQR maturation NqrM -
  ASUC_RS03160 (Asuc_0607) mnmA 670993..672162 (+) 1170 WP_012072361.1 tRNA 2-thiouridine(34) synthase MnmA -
  ASUC_RS03165 (Asuc_0608) - 672211..672477 (-) 267 WP_012072362.1 GNAT family N-acetyltransferase -
  ASUC_RS11340 yacG 672488..672691 (-) 204 WP_245822096.1 DNA gyrase inhibitor YacG -
  ASUC_RS03170 (Asuc_0609) coaE 672689..673306 (-) 618 Protein_611 dephospho-CoA kinase -
  ASUC_RS03175 (Asuc_0610) - 673337..674029 (-) 693 WP_012072364.1 A24 family peptidase -
  ASUC_RS03180 (Asuc_0611) pilC 674026..675237 (-) 1212 WP_012072365.1 type II secretion system F family protein Machinery gene
  ASUC_RS03185 (Asuc_0612) pilB 675227..676669 (-) 1443 WP_012072366.1 GspE/PulE family protein Machinery gene
  ASUC_RS03190 (Asuc_0613) pilA 676681..677136 (-) 456 WP_012072367.1 prepilin peptidase-dependent pilin Machinery gene
  ASUC_RS03195 (Asuc_0614) ampD 677251..677808 (+) 558 WP_012072368.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ASUC_RS03200 (Asuc_0615) - 677881..678339 (-) 459 WP_012072369.1 surface-adhesin E family protein -
  ASUC_RS03205 (Asuc_0616) - 678451..679101 (-) 651 WP_012072370.1 HAD family phosphatase -
  ASUC_RS03210 (Asuc_0617) artM 679257..679943 (-) 687 WP_012072371.1 arginine ABC transporter permease ArtM -
  ASUC_RS03215 (Asuc_0618) artQ 679943..680614 (-) 672 WP_012072372.1 arginine ABC transporter permease ArtQ -
  ASUC_RS03220 (Asuc_0619) - 680618..681340 (-) 723 WP_012072373.1 arginine ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 480 a.a.        Molecular weight: 53890.33 Da        Isoelectric Point: 5.1082

>NTDB_id=28517 ASUC_RS03185 WP_012072366.1 675227..676669(-) (pilB) [Actinobacillus succinogenes 130Z]
MSGDHKTVTTSTGETIEISPHLWTKNQDQQALLVRFLALPLQENDSILWLGLDSLTNLSACETFAFLTGKIIEPVLLTNQ
ELKRCLQALSPQTFQVEENPIFYTEETTDVEQKSDDTVIQLLNQIFENALHRQASDIHLEPRPDCLQVRFRIDGVLQNQT
PISSAFANRVISRLKLLAKLDISEMRLPQDGRFHFKTTFSDVLDLRLSTLPTNLGEKAVLRVQQNKPVTLAFSDLGMNEN
QQRIFRYALSQPQGLILVTGPTGSGKSITLYTALSSLNSAEKHIMTAEDPIEIELDGIIQTQINPAVGLDFSRLLRTFLR
QDPDIIMLGEIRDEESAAMALRAAQTGHLVLSTLHTNDASSAISRLRQLGIQQHEIDSTLLLVIAQRLIRKCCPKCITRP
DDNYLDTEKCGQKSADSCDCHQGYKGRVGIYQLLQPDFSNAQQQYRLDFPDLYQAGLEKINTGITDFTEIQRVLGVKNET

Nucleotide


Download         Length: 1443 bp        

>NTDB_id=28517 ASUC_RS03185 WP_012072366.1 675227..676669(-) (pilB) [Actinobacillus succinogenes 130Z]
ATGTCGGGCGACCACAAAACCGTCACCACAAGTACGGGAGAAACGATAGAAATTTCACCGCATTTGTGGACAAAGAATCA
GGATCAACAAGCGTTACTCGTACGTTTTCTCGCCTTACCTCTGCAGGAAAACGACAGCATACTCTGGCTGGGATTAGACA
GTTTAACCAATTTATCCGCTTGCGAAACCTTTGCATTTTTAACGGGTAAAATCATTGAGCCGGTGCTACTGACCAATCAG
GAATTAAAACGTTGCTTGCAGGCATTATCGCCGCAAACCTTTCAAGTGGAAGAAAATCCGATTTTCTATACCGAGGAAAC
TACCGACGTTGAACAAAAATCCGACGATACCGTTATTCAATTATTGAATCAGATCTTTGAAAACGCCCTTCACCGGCAGG
CTTCGGATATTCATTTGGAACCGCGTCCGGATTGTTTGCAAGTCCGCTTCCGCATTGACGGCGTTTTACAAAACCAAACA
CCGATTTCATCGGCATTTGCCAATCGCGTAATTTCCCGGTTGAAACTGCTGGCGAAATTAGACATCAGTGAAATGCGCCT
GCCGCAGGACGGACGATTCCATTTTAAAACCACCTTTTCCGATGTACTTGATTTGCGCCTCTCCACACTTCCCACTAATC
TGGGGGAAAAGGCGGTACTGCGGGTACAACAAAATAAACCGGTCACGCTGGCATTTTCCGATTTGGGGATGAATGAAAAT
CAACAGCGCATTTTCCGCTATGCCCTCAGTCAACCGCAAGGGCTTATTCTGGTCACCGGCCCTACCGGCAGCGGAAAAAG
TATCACGCTTTATACCGCACTTTCCTCGCTGAATTCTGCCGAAAAACATATTATGACGGCGGAAGATCCCATTGAAATAG
AACTGGACGGGATTATCCAAACCCAAATCAACCCGGCTGTCGGTTTGGATTTTAGTCGTCTGTTGCGAACGTTTCTGCGT
CAGGATCCCGATATCATTATGCTGGGCGAAATTCGTGACGAAGAAAGCGCCGCTATGGCGTTACGTGCCGCGCAAACCGG
TCATTTAGTACTTTCCACTCTGCATACTAACGATGCCTCATCAGCAATTTCGCGTTTACGTCAGCTCGGTATTCAGCAAC
ACGAAATCGACAGCACTCTGCTACTGGTTATCGCACAACGATTAATCAGAAAATGTTGTCCGAAATGCATCACAAGACCG
GACGACAATTATTTGGATACAGAAAAGTGCGGGCAAAAATCCGCTGATTCTTGTGATTGTCATCAAGGTTATAAAGGTCG
TGTGGGAATTTATCAGCTATTACAGCCCGACTTTAGCAACGCTCAACAACAATATCGTTTGGATTTTCCCGACCTTTATC
AGGCGGGGTTGGAAAAAATCAATACGGGAATCACCGATTTTACCGAAATTCAACGGGTACTGGGAGTAAAAAATGAAACT
TAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(124-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A6VLY9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.376

97.5

0.667
  pilB Haemophilus influenzae Rd KW20

68.376

97.5

0.667
  pilB Glaesserella parasuis strain SC1401

55.603

98.542

0.548
  pilB Vibrio campbellii strain DS40M4

41.336

99.792

0.412
  pilB Vibrio parahaemolyticus RIMD 2210633

40.956

100

0.41
  pilB Acinetobacter baylyi ADP1

38.99

100

0.402
  pilB Vibrio cholerae strain A1552

40.208

100

0.402
  pilB Legionella pneumophila strain ERS1305867

38.9

100

0.398
  pilB Acinetobacter baumannii D1279779

37.728

100

0.387

Multiple sequence alignment