Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ASUC_RS03190 Genome accession   NC_009655
Coordinates   676681..677136 (-) Length   151 a.a.
NCBI ID   WP_012072367.1    Uniprot ID   A6VLZ0
Organism   Actinobacillus succinogenes 130Z     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 671681..682136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASUC_RS03165 (Asuc_0608) - 672211..672477 (-) 267 WP_012072362.1 GNAT family N-acetyltransferase -
  ASUC_RS11340 yacG 672488..672691 (-) 204 WP_245822096.1 DNA gyrase inhibitor YacG -
  ASUC_RS03170 (Asuc_0609) coaE 672689..673306 (-) 618 Protein_611 dephospho-CoA kinase -
  ASUC_RS03175 (Asuc_0610) - 673337..674029 (-) 693 WP_012072364.1 A24 family peptidase -
  ASUC_RS03180 (Asuc_0611) pilC 674026..675237 (-) 1212 WP_012072365.1 type II secretion system F family protein Machinery gene
  ASUC_RS03185 (Asuc_0612) pilB 675227..676669 (-) 1443 WP_012072366.1 GspE/PulE family protein Machinery gene
  ASUC_RS03190 (Asuc_0613) pilA 676681..677136 (-) 456 WP_012072367.1 prepilin peptidase-dependent pilin Machinery gene
  ASUC_RS03195 (Asuc_0614) ampD 677251..677808 (+) 558 WP_012072368.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ASUC_RS03200 (Asuc_0615) - 677881..678339 (-) 459 WP_012072369.1 surface-adhesin E family protein -
  ASUC_RS03205 (Asuc_0616) - 678451..679101 (-) 651 WP_012072370.1 HAD family phosphatase -
  ASUC_RS03210 (Asuc_0617) artM 679257..679943 (-) 687 WP_012072371.1 arginine ABC transporter permease ArtM -
  ASUC_RS03215 (Asuc_0618) artQ 679943..680614 (-) 672 WP_012072372.1 arginine ABC transporter permease ArtQ -
  ASUC_RS03220 (Asuc_0619) - 680618..681340 (-) 723 WP_012072373.1 arginine ABC transporter substrate-binding protein -
  ASUC_RS03225 (Asuc_0620) artP 681349..682083 (-) 735 WP_012072374.1 arginine ABC transporter ATP-binding protein ArtP -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 15812.88 Da        Isoelectric Point: 8.0859

>NTDB_id=28518 ASUC_RS03190 WP_012072367.1 676681..677136(-) (pilA) [Actinobacillus succinogenes 130Z]
MKTDRTFYRASFSVSGYKGFTLIELMIVIAIVAILATVAVPSYQNYTKKAAVSELLQASAPYRSEVELCVYNTGSVTNCT
AGSNGIQNDNAGFGKYVDSVKVSGGVITVTGKGSLNDTSYTLTAGGDASKGVTWTTACKPDASIFPSGFCN

Nucleotide


Download         Length: 456 bp        

>NTDB_id=28518 ASUC_RS03190 WP_012072367.1 676681..677136(-) (pilA) [Actinobacillus succinogenes 130Z]
ATGAAAACCGACCGCACTTTTTATCGCGCCTCATTCTCCGTATCCGGGTATAAGGGCTTCACCTTAATCGAATTAATGAT
TGTTATTGCCATTGTCGCGATTTTGGCCACCGTCGCCGTTCCGTCCTATCAAAACTACACCAAAAAAGCCGCCGTTTCGG
AGCTGCTGCAGGCATCGGCACCCTATCGTTCAGAAGTGGAACTCTGTGTCTATAATACCGGTTCCGTCACAAACTGTACG
GCGGGTTCAAACGGCATTCAGAACGATAATGCCGGTTTCGGTAAATATGTAGACAGCGTAAAAGTCTCGGGCGGTGTCAT
TACCGTAACGGGTAAAGGCAGCCTGAACGACACAAGTTACACGTTAACTGCCGGCGGCGACGCGTCTAAAGGCGTAACCT
GGACGACCGCCTGCAAACCGGACGCCAGTATTTTCCCGTCCGGATTCTGTAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(17-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A6VLZ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

67.164

88.742

0.596
  pilA Haemophilus influenzae Rd KW20

65.672

88.742

0.583
  pilA Glaesserella parasuis strain SC1401

52.899

91.391

0.483

Multiple sequence alignment