Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C8I07_RS05820 Genome accession   NZ_CP028355
Coordinates   1354631..1356343 (+) Length   570 a.a.
NCBI ID   WP_107402781.1    Uniprot ID   -
Organism   Shewanella baltica strain CW2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1349631..1361343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C8I07_RS05795 (C8I07_05775) ampE 1350451..1351302 (-) 852 WP_107402778.1 beta-lactamase regulator AmpE -
  C8I07_RS05800 (C8I07_05780) ampD 1351372..1351935 (-) 564 WP_107402779.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C8I07_RS05810 (C8I07_05790) nadC 1352521..1353402 (+) 882 WP_107402780.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C8I07_RS05815 (C8I07_05795) - 1354124..1354570 (+) 447 WP_310795220.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  C8I07_RS05820 (C8I07_05800) pilB 1354631..1356343 (+) 1713 WP_107402781.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C8I07_RS05825 (C8I07_05805) pilC 1356467..1357732 (+) 1266 WP_107402782.1 type II secretion system F family protein Machinery gene
  C8I07_RS05830 (C8I07_05810) pilD 1357776..1358702 (+) 927 WP_107402783.1 prepilin peptidase Machinery gene
  C8I07_RS05835 (C8I07_05815) coaE 1358742..1359359 (+) 618 WP_107402784.1 dephospho-CoA kinase -
  C8I07_RS05840 (C8I07_05820) zapD 1359352..1360086 (+) 735 WP_107402785.1 cell division protein ZapD -
  C8I07_RS05845 (C8I07_05825) yacG 1360160..1360369 (+) 210 WP_006083355.1 DNA gyrase inhibitor YacG -
  C8I07_RS05850 (C8I07_05830) mutT 1360446..1360838 (+) 393 WP_107402786.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62982.35 Da        Isoelectric Point: 5.9851

>NTDB_id=284322 C8I07_RS05820 WP_107402781.1 1354631..1356343(+) (pilB) [Shewanella baltica strain CW2]
MPTTGLHLGLSTLFIRKGLLTEEQMANAITKSRQNKHGLVSTLVQEKLISARAIAELCYEEYGTPLLDLNEFDLTSIPED
FLNKKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEEDKLAKALEKILEEDISGLDISSMDEAA
LAGIEITDTDRRNEEPTGDSSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVSLASRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCSECKQAEVIPEHELLRLGF
AQAQITQGFTTFKPVGCEHCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKAQGMRDLRQSGLLKVLHGVTSI
AEVNRVTSFD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=284322 C8I07_RS05820 WP_107402781.1 1354631..1356343(+) (pilB) [Shewanella baltica strain CW2]
ATGCCAACAACTGGTTTACATCTAGGTTTATCAACCTTATTTATTCGCAAGGGCCTGCTTACCGAGGAGCAGATGGCTAA
CGCCATCACAAAATCCCGTCAGAATAAACATGGACTGGTTAGTACCTTAGTCCAAGAAAAACTCATTTCAGCCCGCGCCA
TTGCTGAGTTGTGTTACGAAGAATATGGCACACCGCTGCTTGACCTTAATGAATTTGATCTTACTTCCATTCCAGAAGAT
TTCCTCAATAAAAAGCTGATTGAAAAACACCGCTGCTTACCCCTGTTTAAACGTGGTAATCGCCTCTATATAGGCACATC
AGACCCGACCAATATTGCCGCCCTCGAAGATTTCCAATTTAGCGCTGGCCTGCACGCCGAAGCCATTCTGGTGGAAGAAG
ATAAACTCGCTAAGGCGCTGGAAAAAATCCTTGAAGAAGACATCAGCGGGCTCGATATCAGTAGCATGGATGAGGCGGCG
CTAGCGGGGATTGAAATTACCGATACAGATAGGCGCAACGAAGAACCCACTGGTGATAGTAGCGATGATGCACCTATCGT
TATTTATATCAATAAAATTTTAACCGATGCAATCCGTAAAGGGGCCTCGGATTTACACTTTGAACCCTATGAGAAACGCT
ACCGTATTCGTTTTCGCATTGACGGTATTCTGCACGAAGTATCAGAACCCCCAGTTAGCCTTGCCAGCCGTTTATCAGCG
CGGTTAAAAGTGATGTCGAAACTCGATATTGCCGAACGGCGTGTACCGCAGGATGGTCGAATTAAGATGAAGCTTTCCCG
CACTAAGTCCATCGACTTTCGGGTAAGTACTCTGCCGACCCTGTGGGGCGAAAAAATTGTAATGCGGATCTTGGACTCAT
CCTCTGCACAATTAGGCATTGAAAAGCTTGGCTACGAGCCTGACCAAGAAAAACTCTATCTTGAGATGCTGGCAAAACCC
CAAGGCATGATTTTAGTGACAGGTCCTACAGGTTCAGGTAAAACCGTCTCCCTTTATACTGGGTTAAACATTCTCAATAC
CGAAGAGCGTAATATTTCTACCGCAGAAGATCCGGTTGAGATTAACCTTGAAGGCGTGAACCAAGTTCACATTAACTTAA
AGGCAGGCCTCACATTCGCATCCGCTTTACGTTCATTCCTACGTCAAGACCCCGATGTGGTGATGGTGGGTGAAATCCGC
GATCTTGAAACCGCCGAGATTGCCATTAAAGCGGCGCAAACGGGTCACTTAGTCTTATCGACCTTGCACACAAACTCGGC
GGCAGAAACCTTAACCCGTTTAATCAACATGGGTGTGCCAGGTTATAACATCGCCAGCTCAGTGAATCTGATTATTGCCC
AGCGCTTAGCCCGTCGCCTCTGCTCTGAATGTAAACAAGCTGAAGTGATACCCGAGCATGAATTACTGCGCCTAGGCTTT
GCCCAAGCGCAAATTACTCAGGGATTTACCACCTTTAAGCCTGTTGGCTGCGAGCATTGCTCCGGCGGCTACAAAGGTCG
GGTCGGTATTTACGAAGTAATGAAGATGTCCGATGAAATAGCCCGTACTATCATGGAGGGTGGTAATTCTTTGCAAATTG
CCTCCCAAGCTAAAGCCCAAGGAATGCGAGATTTACGGCAATCAGGATTATTAAAGGTGCTCCATGGCGTAACGAGTATT
GCTGAAGTCAACCGAGTCACGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.358

98.421

0.584
  pilB Acinetobacter baumannii D1279779

58.511

98.947

0.579
  pilB Legionella pneumophila strain ERS1305867

56.584

98.596

0.558
  pilB Vibrio cholerae strain A1552

53.036

98.246

0.521
  pilB Vibrio parahaemolyticus RIMD 2210633

51.318

99.825

0.512
  pilB Vibrio campbellii strain DS40M4

51.413

99.298

0.511
  pilF Neisseria gonorrhoeae MS11

47.88

99.298

0.475
  pilF Thermus thermophilus HB27

39.153

99.474

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.156

97.018

0.37

Multiple sequence alignment