Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Tharo_RS05365 Genome accession   NZ_CP028339
Coordinates   1114637..1116352 (-) Length   571 a.a.
NCBI ID   WP_107220310.1    Uniprot ID   A0A2R4BL12
Organism   Thauera aromatica K172     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1109637..1121352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Tharo_RS05345 (Tharo_1059) - 1111457..1112083 (-) 627 WP_107220306.1 DUF502 domain-containing protein -
  Tharo_RS05350 (Tharo_1060) - 1112133..1112390 (-) 258 WP_107220307.1 zinc ribbon domain-containing protein -
  Tharo_RS05355 (Tharo_1061) pilD 1112538..1113389 (-) 852 WP_107220308.1 A24 family peptidase Machinery gene
  Tharo_RS05360 (Tharo_1062) pilC 1113393..1114622 (-) 1230 WP_107220309.1 type II secretion system F family protein Machinery gene
  Tharo_RS05365 (Tharo_1063) pilB 1114637..1116352 (-) 1716 WP_107220310.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Tharo_RS05370 (Tharo_1064) - 1116441..1117721 (-) 1281 WP_107220311.1 HlyC/CorC family transporter -
  Tharo_RS05375 (Tharo_1065) ccsA 1117861..1118691 (-) 831 WP_107220312.1 cytochrome c biogenesis protein CcsA -
  Tharo_RS05380 (Tharo_1066) ffh 1118740..1120110 (+) 1371 WP_107220313.1 signal recognition particle protein -
  Tharo_RS05385 (Tharo_1067) - 1120127..1120903 (+) 777 WP_107220314.1 EI24 domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 61679.49 Da        Isoelectric Point: 5.2944

>NTDB_id=284221 Tharo_RS05365 WP_107220310.1 1114637..1116352(-) (pilB) [Thauera aromatica K172]
MAASSKPSLSGLARALIQHGHLQETDALACSAHSGEGSNAFIREVAQRGLMSSAAMAAFAAETFGYPLLDITTFDTAALA
REAVDRKLMQKHQVVPLARRQNRLTLAIADPSNIRALDEIRFQTGLQLDLVIAEADKLGRAVDALVESAADTLRELTGEA
FDMDLLHQEGAQHPPDEDDTSEVDDAPVVRFIQKVLIDAINEGASDIHFEPYEKYYRIRVRTDGVLRDIAQPPLVLKDKI
AARIKVVSRLDISEKRIPQDGRMKLALSKNRAIDFRVSTLPTLHGEKIVMRILDPGSAMLGIDALGYEPDQKEALLAAID
RPYGMILVTGPTGSGKTVSLYTCLNLLNQPGANISTAEDPAEINLPGINQVNINEKAGLTFSTALRAFLRQDPDIIMVGE
IRDLETAEISVKAAQTGHLVMSTVHTNDAPSTLERLKNMGVAPFNIASSVILITAQRLARRLCSCKKPADIPEEALLEAG
FSAAELDGSWQAYGPVGCERCQGSGYKGRVGIYQVMPVSEQIAHIIMTGGNSIDIAAQAAREGVRDLRQSGLLKVKQGIT
SLAEVLASTNE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=284221 Tharo_RS05365 WP_107220310.1 1114637..1116352(-) (pilB) [Thauera aromatica K172]
ATGGCGGCAAGCTCCAAACCGTCCCTGAGCGGCCTCGCCAGGGCGCTGATCCAGCACGGGCATCTGCAGGAAACCGATGC
CCTCGCCTGCTCCGCACACAGCGGAGAAGGCAGCAACGCTTTCATCCGCGAAGTCGCCCAGCGCGGACTCATGAGCAGCG
CGGCGATGGCCGCCTTCGCTGCCGAAACTTTCGGCTACCCCCTGCTCGACATCACCACCTTCGACACCGCCGCCCTCGCC
CGCGAAGCCGTCGATCGCAAGCTGATGCAAAAGCATCAGGTCGTGCCCCTGGCGCGGCGTCAGAACCGCCTCACCCTCGC
AATCGCCGACCCGTCGAACATCCGTGCACTCGACGAGATCCGTTTCCAGACCGGTCTCCAGCTCGACCTCGTGATTGCCG
AAGCCGACAAGCTCGGGCGCGCCGTCGACGCGCTGGTCGAAAGCGCCGCCGATACGCTCCGGGAGCTCACCGGTGAAGCC
TTCGACATGGACCTGCTGCACCAGGAAGGCGCGCAGCACCCGCCCGACGAAGACGACACTTCGGAAGTCGACGACGCGCC
GGTGGTGCGTTTCATCCAGAAAGTGCTGATCGATGCCATCAACGAAGGCGCCTCCGACATCCACTTCGAGCCGTACGAGA
AGTACTACCGCATCCGCGTGCGCACCGACGGCGTCCTGCGCGACATCGCCCAGCCTCCGCTGGTGCTCAAGGACAAGATC
GCCGCCCGCATCAAGGTCGTCTCGCGCCTCGACATCTCGGAAAAGCGCATTCCCCAGGACGGGCGGATGAAGCTGGCGCT
GTCGAAGAACAGGGCGATCGACTTCCGCGTATCCACGCTGCCGACCCTGCACGGCGAAAAGATCGTGATGCGGATCCTCG
ATCCCGGCTCGGCAATGCTCGGCATCGATGCCCTCGGCTACGAGCCCGACCAGAAGGAAGCCCTGCTCGCAGCGATCGAC
CGTCCTTACGGCATGATCCTCGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTCTACACCTGCCTGAACCTGCT
CAACCAGCCCGGCGCCAATATTTCGACCGCGGAAGACCCGGCCGAAATCAACCTCCCCGGAATCAACCAGGTCAACATCA
ACGAAAAAGCCGGCCTCACCTTTTCCACGGCGCTGCGCGCCTTCCTCCGCCAGGACCCGGACATCATCATGGTGGGCGAG
ATCCGCGACCTCGAAACCGCCGAGATCTCGGTCAAGGCGGCACAGACCGGCCACCTCGTGATGTCCACGGTGCACACCAA
CGATGCCCCGAGCACGCTCGAGCGCCTCAAGAACATGGGCGTCGCCCCGTTCAACATCGCCTCTTCGGTGATCCTGATCA
CCGCCCAGCGCCTTGCCCGCCGCCTGTGCTCGTGCAAGAAGCCCGCGGACATCCCGGAAGAGGCGCTGCTCGAAGCCGGC
TTCAGCGCCGCCGAGCTCGACGGCAGCTGGCAGGCCTATGGGCCGGTCGGCTGCGAACGCTGCCAGGGCAGCGGCTACAA
GGGCCGGGTCGGGATCTACCAGGTCATGCCGGTGAGCGAGCAGATCGCCCACATCATCATGACCGGCGGCAACTCCATCG
ACATCGCCGCGCAGGCCGCGCGCGAGGGCGTGCGCGATCTGCGCCAGTCGGGCCTGCTGAAAGTGAAGCAAGGCATCACT
TCGCTCGCCGAAGTGCTGGCCTCGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-148)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R4BL12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.261

100

0.546
  pilB Acinetobacter baylyi ADP1

54.087

100

0.545
  pilB Legionella pneumophila strain ERS1305867

52.622

100

0.527
  pilF Neisseria gonorrhoeae MS11

54.753

92.119

0.504
  pilB Vibrio cholerae strain A1552

52.063

89.142

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

49.43

92.119

0.455
  pilB Vibrio campbellii strain DS40M4

48.859

92.119

0.45
  pilF Thermus thermophilus HB27

39.791

100

0.399
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.414

93.17

0.377

Multiple sequence alignment