Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbB   Type   Machinery gene
Locus tag   LL14B4_RS02020 Genome accession   NZ_CP028160
Coordinates   401126..401515 (+) Length   129 a.a.
NCBI ID   WP_010905314.1    Uniprot ID   S6EQX8
Organism   Lactococcus lactis subsp. lactis strain 14B4     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 401126..404494 401126..401515 within 0


Gene organization within MGE regions


Location: 401126..404494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL14B4_RS02020 (LL14B4_02020) ssbB 401126..401515 (+) 390 WP_010905314.1 single-stranded DNA-binding protein Machinery gene
  LL14B4_RS02025 (LL14B4_02025) groES 401635..401919 (+) 285 WP_003131589.1 co-chaperone GroES -
  LL14B4_RS02030 (LL14B4_02030) groL 402007..403635 (+) 1629 WP_109990650.1 chaperonin GroEL -
  LL14B4_RS02035 (LL14B4_02035) - 403682..404494 (-) 813 WP_003131583.1 MBL fold metallo-hydrolase -

Sequence


Protein


Download         Length: 129 a.a.        Molecular weight: 14672.73 Da        Isoelectric Point: 8.3705

>NTDB_id=281799 LL14B4_RS02020 WP_010905314.1 401126..401515(+) (ssbB) [Lactococcus lactis subsp. lactis strain 14B4]
MNKTMLIGRLTSAPEISKTTNDKSYVRVTLAVNRRFKNEKGEREADFISIIIWGKSAETLVSYAKKGSLISIEGEIRTRN
YTDKQNQKHYVTEILGLSYDLLESRATIALRESAIKTEETLLDAEELPF

Nucleotide


Download         Length: 390 bp        

>NTDB_id=281799 LL14B4_RS02020 WP_010905314.1 401126..401515(+) (ssbB) [Lactococcus lactis subsp. lactis strain 14B4]
ATGAATAAAACCATGTTAATTGGACGCTTAACCAGTGCGCCAGAAATTTCAAAAACAACAAATGATAAATCATATGTCCG
TGTGACCTTAGCAGTCAATCGCCGTTTCAAAAATGAAAAAGGAGAACGAGAGGCAGATTTTATTTCAATTATTATTTGGG
GAAAATCTGCAGAAACTTTAGTTTCTTACGCAAAAAAAGGAAGTCTTATTTCAATTGAAGGAGAAATTAGAACTAGAAAT
TATACGGATAAGCAAAATCAAAAACATTATGTCACAGAAATCTTAGGACTGAGTTATGATTTACTTGAAAGTAGGGCAAC
AATCGCTTTACGAGAAAGCGCTATAAAGACTGAAGAAACCTTACTTGATGCGGAAGAACTCCCTTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB S6EQX8

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Lactococcus lactis subsp. cremoris KW2

90.698

100

0.907

  ssbB Streptococcus sobrinus strain NIDR 6715-7

60.769

100

0.612

  ssbB/cilA Streptococcus pneumoniae TIGR4

56.923

100

0.574

  ssbB/cilA Streptococcus pneumoniae Rx1

56.154

100

0.566

  ssbB/cilA Streptococcus pneumoniae D39

56.154

100

0.566

  ssbB/cilA Streptococcus pneumoniae R6

56.154

100

0.566

  ssbB/cilA Streptococcus mitis NCTC 12261

56.154

100

0.566

  ssbB/cilA Streptococcus mitis SK321

56.154

100

0.566

  ssbA Streptococcus mutans UA159

55.385

100

0.558

  ssb Latilactobacillus sakei subsp. sakei 23K

43.75

86.822

0.38

  ssbA Bacillus subtilis subsp. subtilis str. 168

44.762

81.395

0.364


Multiple sequence alignment