Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C7S18_RS21395 Genome accession   NZ_CP027860
Coordinates   5525401..5527134 (+) Length   577 a.a.
NCBI ID   WP_106893486.1    Uniprot ID   A0A2P1PXK1
Organism   Ahniella affigens strain D13     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5520401..5532134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7S18_RS21380 (C7S18_21375) - 5522361..5523266 (-) 906 WP_106893483.1 DUF2145 domain-containing protein -
  C7S18_RS21385 (C7S18_21380) - 5523263..5523679 (-) 417 WP_146152058.1 hypothetical protein -
  C7S18_RS21390 (C7S18_21385) - 5523883..5525187 (+) 1305 WP_106893485.1 amidohydrolase -
  C7S18_RS21395 (C7S18_21390) pilB 5525401..5527134 (+) 1734 WP_106893486.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C7S18_RS21400 (C7S18_21395) pilC 5527138..5528424 (+) 1287 WP_106893487.1 type II secretion system F family protein Machinery gene
  C7S18_RS21405 (C7S18_21400) - 5528441..5529103 (+) 663 WP_240623941.1 alpha/beta hydrolase -
  C7S18_RS21410 (C7S18_21405) - 5529311..5530003 (-) 693 WP_106893488.1 lipid-binding SYLF domain-containing protein -
  C7S18_RS21415 (C7S18_21410) - 5530137..5531024 (-) 888 WP_106893489.1 hypothetical protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63052.61 Da        Isoelectric Point: 6.0966

>NTDB_id=281055 C7S18_RS21395 WP_106893486.1 5525401..5527134(+) (pilB) [Ahniella affigens strain D13]
MANPINPAQLTGLPGIVRRLVIDGVISEEDARKAFDASNKQKIPLVSFLVQNAVAPAIQVANAAATEFGIPLFDVRALDL
RQAPIKLVEEKLIQQHRAMPLFKRGNRLFVGISDPTNLRALDEIKFQANLPVDPIIVPEDFLEKAIESALAGVDQLNQQL
GGDEDEGLEGMELGDDEDLDDAAQGGNDSGVDDTPIVKFVNKCLLDAIKRGASDIHFEPYETLMRVRFRLDGILRPVANA
PVKLAPRIAARIKVMAGLDIAERRLPQDGRIKLNISKAKSMDFRVSTCPTLFGEKAVLRILDASAAKLGIDKLGYEEEQK
GHFLDAIHKPYGMVLVTGPTGSGKTVSLYTALNILNTDAVNISTAEDPVEIRVHGINQVQQNAKKGMTFAAALRSFLRQD
PDIIMVGEIRDLETAEIAIKAAQTGHFVLSTLHTNDAPQTVARLMNMGIAPFNITSSVTLITAQRLARRLHDCKRPIRIP
REALLAEGFKEDEVDSMTLYEAVGCSGCNDGYKGRVGIYQVMPMTEEIQKVILHGGNAIQIAEIAKRNGINDLRQSALLK
CRNGFTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=281055 C7S18_RS21395 WP_106893486.1 5525401..5527134(+) (pilB) [Ahniella affigens strain D13]
ATGGCGAATCCCATCAATCCGGCACAACTCACCGGCCTGCCCGGCATCGTGCGGCGTTTGGTGATCGATGGGGTCATCAG
TGAGGAAGACGCACGCAAGGCGTTTGATGCGTCTAACAAGCAGAAGATTCCGCTGGTAAGTTTTTTGGTACAAAACGCCG
TGGCGCCGGCCATCCAAGTGGCCAACGCGGCAGCGACCGAATTCGGCATTCCGCTGTTCGATGTGCGTGCGCTCGATCTT
CGGCAGGCGCCGATCAAGCTGGTGGAGGAAAAACTGATCCAGCAGCATCGGGCGATGCCTTTGTTCAAGCGCGGCAATCG
CCTGTTCGTCGGCATTTCTGATCCAACCAACCTGCGTGCGCTGGACGAAATTAAATTCCAGGCCAATCTGCCGGTCGACC
CCATCATTGTGCCCGAGGACTTCCTGGAGAAGGCCATCGAATCGGCGTTGGCCGGCGTCGACCAGCTGAATCAGCAGCTG
GGTGGCGATGAGGACGAAGGCCTGGAGGGCATGGAGCTCGGTGACGACGAAGATCTGGACGATGCGGCCCAGGGTGGTAA
CGACTCCGGGGTCGATGACACGCCGATCGTCAAGTTCGTCAACAAGTGCCTGCTCGACGCGATCAAGCGCGGCGCGTCCG
ATATTCACTTCGAGCCTTACGAGACGTTGATGCGCGTGCGCTTTCGTCTGGACGGCATTCTGCGGCCGGTCGCCAACGCG
CCGGTCAAGCTGGCGCCGCGCATTGCCGCCCGTATCAAGGTCATGGCTGGTCTGGACATTGCCGAGCGCCGTCTCCCGCA
GGACGGACGCATCAAGCTGAATATCAGCAAGGCGAAGTCGATGGACTTTCGCGTGAGTACCTGCCCAACGCTGTTCGGCG
AAAAAGCGGTGCTGCGTATTCTGGACGCGTCCGCGGCCAAGCTCGGTATCGACAAGCTGGGCTATGAAGAAGAGCAGAAA
GGTCACTTCCTGGATGCCATTCACAAGCCCTATGGAATGGTGCTGGTGACCGGTCCAACCGGGTCCGGCAAAACGGTATC
GCTCTATACCGCGCTCAATATTCTCAATACCGATGCGGTCAATATCTCGACGGCTGAAGACCCGGTCGAAATCCGCGTGC
ATGGCATCAACCAGGTTCAGCAGAATGCCAAGAAGGGCATGACGTTCGCGGCGGCGCTGCGCTCGTTCCTGCGCCAGGAC
CCGGACATCATCATGGTCGGCGAAATCCGCGATCTGGAAACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGGCACTT
CGTGTTGTCCACGCTGCACACCAACGACGCGCCGCAGACGGTTGCGCGCCTGATGAACATGGGCATTGCCCCGTTCAATA
TCACCTCATCTGTGACCTTGATCACGGCGCAGCGCCTGGCGCGGCGCTTACATGATTGCAAACGGCCCATCCGAATCCCG
CGCGAGGCCCTGCTGGCTGAAGGCTTCAAGGAAGACGAGGTTGACTCGATGACCTTGTACGAAGCCGTTGGCTGCAGCGG
CTGCAACGATGGTTACAAGGGTCGCGTCGGCATTTATCAAGTGATGCCGATGACCGAAGAAATTCAGAAGGTGATTCTGC
ATGGCGGCAACGCCATCCAGATCGCCGAAATTGCCAAGCGCAACGGCATCAACGATCTGCGCCAGTCTGCACTGCTGAAA
TGCCGTAACGGCTTCACCAGTCTGGCCGAGATCAACCGCGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)

Type II/IV secretion system protein (T2SSE)

(202-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2P1PXK1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.386

98.787

0.537
  pilB Legionella pneumophila strain ERS1305867

54.021

99.133

0.536
  pilB Acinetobacter baylyi ADP1

53.603

98.614

0.529
  pilF Neisseria gonorrhoeae MS11

49.129

99.48

0.489
  pilB Vibrio cholerae strain A1552

48.873

100

0.489
  pilB Vibrio campbellii strain DS40M4

45.172

100

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

45.826

93.414

0.428
  pilF Thermus thermophilus HB27

38.681

97.227

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.456

98.094

0.367

Multiple sequence alignment