Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C7H73_RS13855 Genome accession   NZ_CP027792
Coordinates   3027374..3029110 (+) Length   578 a.a.
NCBI ID   WP_106847186.1    Uniprot ID   -
Organism   Pulveribacter suum strain SC2-7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3022374..3034110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7H73_RS13825 (C7H73_13805) proB 3022937..3024079 (-) 1143 WP_106847181.1 glutamate 5-kinase -
  C7H73_RS13830 (C7H73_13810) cgtA 3024159..3025238 (-) 1080 WP_106847182.1 Obg family GTPase CgtA -
  C7H73_RS13835 (C7H73_13815) rpmA 3025333..3025590 (-) 258 WP_106847183.1 50S ribosomal protein L27 -
  C7H73_RS13840 (C7H73_13820) rplU 3025609..3025920 (-) 312 WP_106847184.1 50S ribosomal protein L21 -
  C7H73_RS13845 (C7H73_13825) - 3026102..3027085 (+) 984 WP_227001356.1 polyprenyl synthetase family protein -
  C7H73_RS13855 (C7H73_13835) pilB 3027374..3029110 (+) 1737 WP_106847186.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C7H73_RS13860 (C7H73_13840) pilC 3029135..3030352 (+) 1218 WP_106847187.1 type II secretion system F family protein Machinery gene
  C7H73_RS13865 (C7H73_13845) pilD 3030352..3031239 (+) 888 WP_106847188.1 A24 family peptidase Machinery gene
  C7H73_RS13870 (C7H73_13850) coaE 3031314..3031856 (+) 543 WP_264371566.1 dephospho-CoA kinase -
  C7H73_RS13875 (C7H73_13855) zapD 3031908..3032663 (+) 756 WP_106847189.1 cell division protein ZapD -
  C7H73_RS13880 (C7H73_13860) - 3032675..3032890 (+) 216 WP_106847190.1 DNA gyrase inhibitor YacG -
  C7H73_RS15915 - 3032878..3032979 (-) 102 WP_157948355.1 DUF1330 domain-containing protein -
  C7H73_RS13890 (C7H73_13870) - 3033178..3033876 (-) 699 WP_106847191.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62921.18 Da        Isoelectric Point: 5.3382

>NTDB_id=280796 C7H73_RS13855 WP_106847186.1 3027374..3029110(+) (pilB) [Pulveribacter suum strain SC2-7]
MAAVNTPQKEAQPIALPGLARALMSVGKLTQQSAEEVYKKSQASRASFIATLTAAGSITPAEVAHTVSSVFGAPLLDIDA
LDPLRISKELLDIKLCQAYGVVALSKRSNRLIVATADPTNQEAAEKIKFTTQMGVDWVIAEHDKLLRLIEATAKGGPESM
DAIVSGGDFEFDDTSMEAAAEEEDAQTNEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHHYRVRFRVDGELREIASPP
IAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIEALGYEPEEKE
RLLRAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNERAGLTFATALKAFLRQDP
DIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPNCKEPADIP
HESLLDAGFSEEEIDGSWTPYRPVGCSACNNGYKGRVGIYEVMPITEDLQRIILADGSALEIAAQARLEGVRSLRQSGLH
KVKTGMTSLEEVLGVTNE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=280796 C7H73_RS13855 WP_106847186.1 3027374..3029110(+) (pilB) [Pulveribacter suum strain SC2-7]
ATGGCTGCCGTCAATACTCCTCAAAAAGAAGCCCAGCCTATCGCGCTGCCGGGCTTGGCGCGCGCATTGATGTCGGTTGG
AAAACTGACGCAACAATCAGCCGAAGAAGTTTACAAAAAATCACAGGCTTCGCGAGCCAGTTTCATTGCCACTTTGACGG
CTGCCGGCAGCATCACGCCGGCAGAAGTGGCGCACACCGTGTCCAGCGTCTTCGGAGCCCCACTGCTGGACATTGACGCG
CTCGATCCGCTGCGTATCTCCAAGGAATTGCTGGATATCAAGCTGTGCCAAGCCTACGGTGTCGTGGCCCTGAGCAAGCG
CAGCAACCGGCTGATCGTCGCCACGGCGGATCCGACCAATCAGGAGGCTGCAGAGAAGATCAAGTTCACCACGCAAATGG
GCGTGGATTGGGTGATTGCCGAACACGACAAACTGCTGCGCTTGATCGAGGCTACCGCCAAGGGCGGGCCGGAGTCGATG
GATGCCATCGTTAGCGGCGGCGACTTCGAGTTTGACGACACCTCGATGGAGGCAGCGGCCGAAGAAGAGGACGCCCAGAC
GAATGAGGTCGAAGACGCGCCCATCGTCAAGTTTTTGCACAAGATGCTGCTCGATGCGTTCAACATGCGCGCGTCCGATT
TGCACTTCGAACCTTACGAGCATCACTACCGGGTGCGCTTTCGCGTCGATGGCGAGTTGCGCGAGATTGCATCGCCGCCC
ATTGCCATTAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCGCGCCTGGACATCTCCGAAAAGCGCGTGCCCCAGGA
TGGCCGCATGAAGCTGAAAGTAGGCCCGGACCGGGTGATCGACTTTCGCGTGAGCACGCTGCCCACCCTGTTCGGCGAGA
AGATCGTGATCCGTATCCTGGACCCCAGCAGCGCCAAGCTGGGCATTGAAGCGCTGGGCTACGAGCCGGAGGAGAAGGAG
CGGCTGCTGCGTGCCATCGGCCGCCCCTACGGAATGATCCTGGTGACGGGGCCTACCGGTTCGGGCAAGACGGTGTCCCT
CTATACCTGCCTGAATCTGTTGAACAAGCCGGGCGTGAATATCGCTACAGCCGAAGACCCTTCGGAAATCAACCTGCCCG
GCGTGAACCAGGTCAACGTCAATGAAAGGGCCGGCCTCACTTTCGCCACAGCGCTGAAGGCATTTCTGCGCCAGGATCCG
GACATCATCATGGTGGGTGAAATCCGTGACCTGGAAACGGCGGACATTTCCATCAAGGCGGCGCAGACCGGTCACCTGGT
TCTGTCCACGCTGCACACCAACGACGCGCCCACCACGCTCACGCGCATGCGCAACATGGGCATTGCGCCGTTCAACATCG
CCTCCAGCGTGATTTTGATCACCGCACAACGCCTGGCACGTCGCCTGTGCCCCAATTGCAAGGAGCCGGCCGACATTCCG
CACGAATCATTGCTGGATGCCGGCTTCAGCGAGGAAGAGATCGATGGGTCCTGGACACCCTACCGGCCGGTGGGCTGTTC
AGCATGCAACAACGGCTACAAGGGCCGGGTGGGCATCTACGAGGTGATGCCGATCACTGAAGATTTGCAGCGCATCATCC
TGGCCGACGGCAGCGCTCTGGAAATCGCTGCCCAGGCGCGGCTCGAGGGAGTGCGCTCGCTGCGCCAGTCCGGGCTGCAC
AAAGTAAAAACGGGAATGACGTCGCTGGAGGAAGTCCTGGGCGTCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-151)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.925

100

0.547
  pilB Acinetobacter baumannii D1279779

52.84

100

0.531
  pilF Neisseria gonorrhoeae MS11

52.482

97.578

0.512
  pilB Legionella pneumophila strain ERS1305867

50

97.578

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

45.76

97.924

0.448
  pilB Vibrio campbellii strain DS40M4

45.31

97.751

0.443
  pilB Vibrio cholerae strain A1552

46.705

89.273

0.417
  pilF Thermus thermophilus HB27

39.826

99.481

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.753

97.924

0.389

Multiple sequence alignment