Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4K04_RS02305 Genome accession   NZ_CP027753
Coordinates   504649..506721 (+) Length   690 a.a.
NCBI ID   WP_124318831.1    Uniprot ID   A0A3G7TGQ7
Organism   Pseudomonas chlororaphis strain B25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 499649..511721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K04_RS02285 (C4K04_0464) pilM 501879..502931 (+) 1053 WP_124318827.1 pilus assembly protein PilM Machinery gene
  C4K04_RS02290 (C4K04_0465) - 502931..503497 (+) 567 WP_124318828.1 PilN domain-containing protein -
  C4K04_RS02295 (C4K04_0466) pilO 503494..504111 (+) 618 WP_124318829.1 type 4a pilus biogenesis protein PilO -
  C4K04_RS02300 (C4K04_0467) - 504108..504635 (+) 528 WP_124318830.1 pilus assembly protein PilP -
  C4K04_RS02305 (C4K04_0468) pilQ 504649..506721 (+) 2073 WP_124318831.1 type IV pilus secretin PilQ Machinery gene
  C4K04_RS02310 (C4K04_0469) aroK 506726..507244 (+) 519 WP_009041824.1 shikimate kinase AroK -
  C4K04_RS02315 (C4K04_0470) aroB 507393..508493 (+) 1101 WP_016704447.1 3-dehydroquinate synthase -
  C4K04_RS02320 (C4K04_0471) - 508504..510093 (+) 1590 WP_124318832.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74627.72 Da        Isoelectric Point: 5.4337

>NTDB_id=280090 C4K04_RS02305 WP_124318831.1 504649..506721(+) (pilQ) [Pseudomonas chlororaphis strain B25]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLANKSRD
LGNGNARSVTVVESGPRTRVVVNLVALAPYSTRIAGNQLFVVIGQGATAAQSAPGQPAVASGRAKASASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=280090 C4K04_RS02305 WP_124318831.1 504649..506721(+) (pilQ) [Pseudomonas chlororaphis strain B25]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGGCCGGTGCCCGAGCCGCGGG
GTTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAGCTGGCGAACAAGAGCCGCGAT
CTGGGCAATGGCAATGCGCGCAGCGTGACAGTGGTCGAATCGGGGCCGCGTACCCGGGTGGTGGTCAACCTGGTCGCGCT
GGCGCCCTACAGCACTCGCATCGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACAGCGGCGCAGAGTGCAC
CGGGGCAGCCGGCTGTCGCGTCCGGGCGGGCCAAGGCCTCGGCTTCGGCGGGGCGGTCGATTCGCAGCGTGGATTTCCAG
CGCGGCGAGCAGGGTGAAGGCAATGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATCCAGGAGCGCGACGG
CAAGCTCGTCCTTACCTTCGCCAAGGCCCGCCTGCCAGAACCCCTGCGGGTCAGGCTGGACGTCAAGGACTTCGCCACTC
CCGTGCAATTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATTGTCACCATCGAGCCTGGCGGTACCTTCGATTACTCG
ACCTATCAGACGGACAACAAGCTCACGGTAAGCGTCAGGCCGATGACTTCGGGTGATCTGCAGAAACGCAACGCCGAGCG
CTCGGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGTCTGCAGAATGTGCCGTGGGACCAG
GCTCTCGATCTGGTGCTCAAGACGAAGGGGCTGGACAAGCGCAAGGTGGGCAATGTGTTGCTGGTGGCGCCAGCCGATGA
AATCGCGGCCCGCGAACGCCAGGAGCTGGAATCGCAGAAGCAGATAGCCGACCTGGAGCCGCTGCGGCGCGAACTGCTGC
AGGTCAACTACGCCAAGGCCGCGGAGATCGCCAAGCTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGTCCGACGAGCGT
GGTTCGATCACGGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTTGGCGTGCGCTGGGGAGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAATGGTACCAATGGC
TCTTCCACCATAGGCACGCCAGGCAGTACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAGCTGACAGCCATGGAAAAGACCGGTAACGGTGAAA
TCGTCTCCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGTACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCGACTTCGGTGTCGTTCAAGGAGGCTTCGCTGTCCCTGGAAGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTCATCGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTAGGCGATGTGCCGTATCTTGGCCGCCTTTTCAGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-122)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(298-344)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G7TGQ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.769

100

0.733
  comQ Acinetobacter baylyi ADP1

39.918

100

0.422
  pilQ Acinetobacter baumannii strain A118

37.718

100

0.407
  pilQ Acinetobacter baumannii D1279779

37.718

100

0.407
  pilQ Legionella pneumophila strain Lp02

38.039

97.536

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.117

96.957

0.37

Multiple sequence alignment