Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C4K04_RS02285 Genome accession   NZ_CP027753
Coordinates   501879..502931 (+) Length   350 a.a.
NCBI ID   WP_124318827.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain B25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 496879..507931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K04_RS02275 (C4K04_0461) - 497821..499089 (+) 1269 WP_007925851.1 malic enzyme-like NAD(P)-binding protein -
  C4K04_RS02280 (C4K04_0462) - 499227..501662 (-) 2436 WP_206429827.1 penicillin-binding protein 1A -
  C4K04_RS02285 (C4K04_0464) pilM 501879..502931 (+) 1053 WP_124318827.1 pilus assembly protein PilM Machinery gene
  C4K04_RS02290 (C4K04_0465) - 502931..503497 (+) 567 WP_124318828.1 PilN domain-containing protein -
  C4K04_RS02295 (C4K04_0466) pilO 503494..504111 (+) 618 WP_124318829.1 type 4a pilus biogenesis protein PilO -
  C4K04_RS02300 (C4K04_0467) - 504108..504635 (+) 528 WP_124318830.1 pilus assembly protein PilP -
  C4K04_RS02305 (C4K04_0468) pilQ 504649..506721 (+) 2073 WP_124318831.1 type IV pilus secretin PilQ Machinery gene
  C4K04_RS02310 (C4K04_0469) aroK 506726..507244 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37105.57 Da        Isoelectric Point: 4.8956

>NTDB_id=280089 C4K04_RS02285 WP_124318827.1 501879..502931(+) (pilM) [Pseudomonas chlororaphis strain B25]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLSDDDRERQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARMVDLEVCALERSFALAAGASVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSQEEAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQCLGLPVLIANPFAGM
GIGARVDAAALVSDAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=280089 C4K04_RS02285 WP_124318827.1 501879..502931(+) (pilM) [Pseudomonas chlororaphis strain B25]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGTTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGTGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCTGAAGGGGTGGGGCATGCCCTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGTGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTGTCCGACGACGATCGGGAACGCCAGCT
CAAGGTCGACGCCGACCAGTACATTCCCTATCCGCTGGACGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAGCACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCACGGATGGTGGACCTGGAAGTTTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTCAGTGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGCAAGAAGAGGCCGGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGACTTGCGCCGTTGATCGAGCAGTGCCTGGGCTTGCCCGTGCTGATTGCCAACCCCTTCGCTGGCATG
GGCATCGGCGCCAGGGTCGACGCCGCGGCCCTGGTCAGCGACGCCCCGTCCTTGATGATCGCCTGCGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.25

100

0.566
  comM Acinetobacter nosocomialis M2

55.966

100

0.563
  comM Acinetobacter baylyi ADP1

52.557

100

0.529
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.454

Multiple sequence alignment