Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4K17_RS02345 Genome accession   NZ_CP027746
Coordinates   520418..522490 (+) Length   690 a.a.
NCBI ID   WP_081362897.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain DSM 19603     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 515418..527490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K17_RS02325 (C4K17_0466) pilM 517648..518700 (+) 1053 WP_081362901.1 pilus assembly protein PilM Machinery gene
  C4K17_RS02330 (C4K17_0467) - 518700..519266 (+) 567 WP_081362900.1 PilN domain-containing protein -
  C4K17_RS02335 (C4K17_0468) pilO 519263..519880 (+) 618 WP_081362899.1 type 4a pilus biogenesis protein PilO -
  C4K17_RS02340 (C4K17_0469) - 519877..520404 (+) 528 WP_081362898.1 pilus assembly protein PilP -
  C4K17_RS02345 (C4K17_0470) pilQ 520418..522490 (+) 2073 WP_081362897.1 type IV pilus secretin PilQ Machinery gene
  C4K17_RS02350 (C4K17_0471) aroK 522495..523013 (+) 519 WP_009041824.1 shikimate kinase AroK -
  C4K17_RS02355 (C4K17_0472) aroB 523161..524261 (+) 1101 WP_016704447.1 3-dehydroquinate synthase -
  C4K17_RS02360 (C4K17_0473) - 524272..525861 (+) 1590 WP_016704448.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74598.68 Da        Isoelectric Point: 5.4337

>NTDB_id=279936 C4K17_RS02345 WP_081362897.1 520418..522490(+) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain DSM 19603]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYATDQPARIALDLPGVVSQLANKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAAQSAPGQPAVASGRAKAAASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=279936 C4K17_RS02345 WP_081362897.1 520418..522490(+) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain DSM 19603]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGGCCGGTGCCCGAGCCGCGGG
GTTACGCCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAGCTGGCGAACAAGAGCCGCGAT
CTGGGCAATGGCAATGCACGCAGCGTGACAGTGGTCGAATCGGGGCAGCGTACCCGGGTGGTGGTCAACCTGGTCGCGCT
GGCGCCCTACAGTACTCGAGTTGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCAGAGTGCTC
CGGGGCAGCCGGCTGTCGCATCCGGGCGGGCCAAGGCTGCGGCTTCGGCGGGGCGGTCGATTCGCAGTGTGGATTTTCAG
CGCGGCGAGCAGGGTGAGGGCAATGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATCCAGGAACGCGACGG
CAAGCTCGTCCTCACCTTTGCCAAGGCCCGCCTGCCAGAGCCCCTGCGGGTCAGGCTGGACGTCAAGGACTTCGCCACTC
CCGTGCAATTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGTACCTTCGATTACTCG
ACCTACCAGACGGACAACAAGCTCACGGTAAGCGTCAGGCCCATGACTTCGGGCGATCTGCAGAAACGCAATGCCGAGCG
CTCGGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATAGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAATCTCAACCTGGTGGCCAGCGACACGGTGCAGGGTGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCTCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAACGCAAGGTGGGCAATGTGCTGCTGGTGGCGCCGGCCGACGA
AATCGCGGCCCGCGAACGCCAGGAACTGGAGTCGCAGAAGCAGATAGCCGATTTGGAGCCGCTGCGGCGCGAACTGCTGC
AGGTCAACTATGCCAAGGCGGCGGAAATCGCCAAGCTGTTCCAGTCGGTCACCCGCGCTGAGGACAAGTCCGACGAGCGT
GGGTCGATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGTGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGTACCAACGGC
TCTTCCACCATAGGCACGCCGGGCAGCACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAGCTGACAGCCATGGAAAAGACCGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTGGTCACCTCCGACAAAGAGACGGCGAAGATTCTCAAGGGTACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCAACTTCGGTGTCGTTCAAGGAGGCGTCGCTGTCCCTGGAGGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTTATCGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTAGGCGATGTGCCGTATCTTGGCCGCCTTTTCAGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(298-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.769

100

0.733
  comQ Acinetobacter baylyi ADP1

40.055

100

0.423
  pilQ Acinetobacter baumannii strain A118

37.987

100

0.41
  pilQ Acinetobacter baumannii D1279779

37.987

100

0.41
  pilQ Legionella pneumophila strain Lp02

38.039

97.536

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.117

96.957

0.37

Multiple sequence alignment