Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C4K17_RS02325 Genome accession   NZ_CP027746
Coordinates   517648..518700 (+) Length   350 a.a.
NCBI ID   WP_081362901.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain DSM 19603     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 512648..523700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K17_RS02315 (C4K17_0463) - 513600..514868 (+) 1269 WP_007925851.1 malic enzyme-like NAD(P)-binding protein -
  C4K17_RS02320 (C4K17_0464) - 514996..517431 (-) 2436 WP_164485365.1 penicillin-binding protein 1A -
  C4K17_RS02325 (C4K17_0466) pilM 517648..518700 (+) 1053 WP_081362901.1 pilus assembly protein PilM Machinery gene
  C4K17_RS02330 (C4K17_0467) - 518700..519266 (+) 567 WP_081362900.1 PilN domain-containing protein -
  C4K17_RS02335 (C4K17_0468) pilO 519263..519880 (+) 618 WP_081362899.1 type 4a pilus biogenesis protein PilO -
  C4K17_RS02340 (C4K17_0469) - 519877..520404 (+) 528 WP_081362898.1 pilus assembly protein PilP -
  C4K17_RS02345 (C4K17_0470) pilQ 520418..522490 (+) 2073 WP_081362897.1 type IV pilus secretin PilQ Machinery gene
  C4K17_RS02350 (C4K17_0471) aroK 522495..523013 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37023.44 Da        Isoelectric Point: 4.9966

>NTDB_id=279935 C4K17_RS02325 WP_081362901.1 517648..518700(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain DSM 19603]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGASVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSKEDAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVSGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=279935 C4K17_RS02325 WP_081362901.1 517648..518700(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain DSM 19603]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGTGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGATGGCTGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTTTGCGATGACGACCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGACGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGACGCTGGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGATTACGCCAGTGAAGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAACA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTTCGGGACTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCCGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTTGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.83

100

0.551
  comM Acinetobacter nosocomialis M2

54.545

100

0.549
  comM Acinetobacter baylyi ADP1

52.273

100

0.526
  pilM Legionella pneumophila strain ERS1305867

45.198

100

0.457

Multiple sequence alignment