Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C4K22_RS26945 Genome accession   NZ_CP027741
Coordinates   5985490..5986707 (+) Length   405 a.a.
NCBI ID   WP_124312163.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain 449     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5980490..5991707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K22_RS26930 (C4K22_5484) - 5980935..5983862 (+) 2928 WP_124335559.1 DUF748 domain-containing protein -
  C4K22_RS26935 (C4K22_5485) - 5984065..5984421 (-) 357 WP_009050906.1 BON domain-containing protein -
  C4K22_RS26940 (C4K22_5486) - 5984709..5985125 (-) 417 WP_096375478.1 pilin -
  C4K22_RS26945 (C4K22_5487) pilC 5985490..5986707 (+) 1218 WP_124312163.1 type II secretion system F family protein Machinery gene
  C4K22_RS26950 (C4K22_5488) pilD 5986710..5987582 (+) 873 WP_009050909.1 A24 family peptidase Machinery gene
  C4K22_RS26955 (C4K22_5489) coaE 5987579..5988202 (+) 624 WP_124312164.1 dephospho-CoA kinase -
  C4K22_RS26960 (C4K22_5490) yacG 5988199..5988402 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  C4K22_RS26965 (C4K22_5491) - 5988407..5988622 (-) 216 WP_009050911.1 hypothetical protein -
  C4K22_RS26970 (C4K22_5492) - 5988688..5989377 (-) 690 WP_124312165.1 energy-coupling factor ABC transporter permease -
  C4K22_RS26975 (C4K22_5493) - 5989374..5989841 (-) 468 WP_124312166.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44400.20 Da        Isoelectric Point: 10.4090

>NTDB_id=279842 C4K22_RS26945 WP_124312163.1 5985490..5986707(+) (pilC) [Pseudomonas chlororaphis subsp. aurantiaca strain 449]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLVRAQLRKQGISPARVRKKSSSLLQRTPPIKARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLLAELKQDIAAGSSFTSALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRVRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQHWWWLLAGALLLSVFGLR
HAHRRSPRFRHWLDAGLLKLPLVAPLLYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPMIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=279842 C4K22_RS26945 WP_124312163.1 5985490..5986707(+) (pilC) [Pseudomonas chlororaphis subsp. aurantiaca strain 449]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGGTCAGGGCCCAGTTGCGCAAGCAAGGCATCAGCCCGGCCAGGGTCAGGAAGAAATCCA
GCTCGCTGTTGCAGCGCACGCCCCCGATCAAGGCCCGCGACATCAGCCTGTTCACCCGGCAGTTGGCGACCCTGATCAAG
GCCGGCGTACCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGATAACCCGAACATGCGCAAACTGTTGGCCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGTTTCACCAGTGCGCTGCGCAAGAGGCCGCGGTATTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAGCAGGCCGGCGCCCTGGAAACCCTGCTGGAGCGAGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACCCGAGTCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTCACGGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAAGGCTTCGGCGCGCAACTGCCGGTGTTCACCCAACTGG
TCATCGCCCTCTCGGAATTCGTGCAGCACTGGTGGTGGCTGCTGGCGGGCGCTCTGTTGCTCAGTGTGTTCGGCCTGCGC
CATGCCCACCGGCGCTCACCGCGCTTTCGCCACTGGCTGGACGCGGGCCTGCTGAAACTGCCATTGGTGGCGCCGCTGCT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCGCTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAATCAGGTGTTCAAGCATGCGGTGAACCGCATCAGGCAAGACGTGGCGACCGGCATG
CAGTTGCACTTTTCCATGCGCGCGTCCGGCATTTTTCCCGGCATGGCGATCCAGATGACCGCCATCGGCGAAGAATCCGG
GGCCCTGGACGGCATGCTGGAAAAAGTCGCGATCCACTACGAGGAAGAGGTCGATACCCTGGTCGATACCCTGAGCAGTC
TCATGGAGCCCATGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.926

100

0.659
  pilC Acinetobacter baylyi ADP1

55.392

100

0.558
  pilC Acinetobacter baumannii D1279779

55.147

100

0.556
  pilC Legionella pneumophila strain ERS1305867

52.273

97.778

0.511
  pilG Neisseria gonorrhoeae MS11

43.176

99.506

0.43
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio cholerae strain A1552

43.367

96.79

0.42
  pilC Vibrio campbellii strain DS40M4

40.05

99.259

0.398

Multiple sequence alignment