Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C4K33_RS26100 Genome accession   NZ_CP027736
Coordinates   5867109..5868326 (+) Length   405 a.a.
NCBI ID   WP_063433050.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. piscium strain PCL1391     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5862109..5873326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K33_RS26085 (C4K33_5299) - 5862566..5865481 (+) 2916 WP_173678550.1 DUF748 domain-containing protein -
  C4K33_RS26090 (C4K33_5300) - 5865684..5866040 (-) 357 WP_025807489.1 BON domain-containing protein -
  C4K33_RS26095 (C4K33_5301) - 5866329..5866745 (-) 417 WP_063433049.1 pilin -
  C4K33_RS26100 (C4K33_5302) pilC 5867109..5868326 (+) 1218 WP_063433050.1 type II secretion system F family protein Machinery gene
  C4K33_RS26105 (C4K33_5303) pilD 5868329..5869201 (+) 873 WP_063433051.1 A24 family peptidase Machinery gene
  C4K33_RS26110 (C4K33_5304) coaE 5869198..5869821 (+) 624 WP_063433052.1 dephospho-CoA kinase -
  C4K33_RS26115 (C4K33_5305) yacG 5869818..5870021 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  C4K33_RS26120 (C4K33_5306) - 5870026..5870241 (-) 216 WP_025807496.1 hypothetical protein -
  C4K33_RS26125 (C4K33_5307) - 5870307..5870996 (-) 690 WP_063433053.1 energy-coupling factor ABC transporter permease -
  C4K33_RS26130 (C4K33_5308) - 5870993..5871460 (-) 468 WP_063433054.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44579.39 Da        Isoelectric Point: 10.4288

>NTDB_id=279706 C4K33_RS26100 WP_063433050.1 5867109..5868326(+) (pilC) [Pseudomonas chlororaphis subsp. piscium strain PCL1391]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGISPARVRKKSRSLFQRTPPIRARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLMAELKQDIAAGSSFTTALRKRPRYFDELYCSLVDAGEQAGALETLLDRVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQHWWWLLAGGLLLSVFGLR
HAHRHSPRFRQWLDVGLLKLPLVAPLMYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDSMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=279706 C4K33_RS26100 WP_063433050.1 5867109..5868326(+) (pilC) [Pseudomonas chlororaphis subsp. piscium strain PCL1391]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAGCCCGGCCAGGGTCAGGAAGAAATCCC
GTTCGCTGTTCCAGCGCACGCCCCCGATCAGGGCCCGCGACATCAGCTTGTTCACCCGGCAGCTGGCGACCCTGATCAAG
GCCGGCGTGCCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGACAACCCGAACATGCGCAAACTGATGGCCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCACAGCATTGCGCAAGAGGCCGCGGTACTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAACAGGCCGGGGCCCTGGAAACCCTGCTGGATCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACGCGCATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTCACGGGCAT
TCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAAGGCTTCGGCGCGCAACTGCCGGTGTTCACCCAACTGG
TCATCGCCCTCTCGGAGTTCGTGCAGCACTGGTGGTGGCTGTTGGCGGGCGGTCTGTTGCTCAGCGTGTTCGGCCTGCGC
CATGCCCACCGGCACTCACCGCGCTTTCGCCAGTGGCTGGACGTGGGCCTGCTGAAACTGCCGTTGGTAGCGCCGCTGAT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCACTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAATCAGGTGTTCAAGCATGCGGTGAACCGCATCAGGCAGGACGTGGCGACTGGCATG
CAGTTGCACTTTTCCATGCGCGCGTCCGGCATCTTTCCCGGCATGGCCATCCAGATGACCGCCATCGGCGAAGAGTCCGG
GGCCCTGGACAGCATGCTGGAAAAGGTCGCGATCCACTATGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAACCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGTACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.432

100

0.654
  pilC Acinetobacter baylyi ADP1

55.637

100

0.56
  pilC Acinetobacter baumannii D1279779

54.902

100

0.553
  pilC Legionella pneumophila strain ERS1305867

53.03

97.778

0.519
  pilG Neisseria gonorrhoeae MS11

41.542

99.259

0.412
  pilG Neisseria meningitidis 44/76-A

40.796

99.259

0.405
  pilC Vibrio cholerae strain A1552

41.327

96.79

0.4
  pilC Vibrio campbellii strain DS40M4

38.308

99.259

0.38

Multiple sequence alignment