Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4K33_RS02270 Genome accession   NZ_CP027736
Coordinates   503057..505129 (+) Length   690 a.a.
NCBI ID   WP_063427909.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. piscium strain PCL1391     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 498057..510129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K33_RS02250 (C4K33_0453) pilM 500286..501338 (+) 1053 WP_063427902.1 pilus assembly protein PilM Machinery gene
  C4K33_RS02255 (C4K33_0454) - 501338..501904 (+) 567 WP_025808191.1 PilN domain-containing protein -
  C4K33_RS02260 (C4K33_0455) pilO 501901..502518 (+) 618 WP_063427904.1 type 4a pilus biogenesis protein PilO -
  C4K33_RS02265 (C4K33_0456) - 502515..503060 (+) 546 WP_063427907.1 pilus assembly protein PilP -
  C4K33_RS02270 (C4K33_0457) pilQ 503057..505129 (+) 2073 WP_063427909.1 type IV pilus secretin PilQ Machinery gene
  C4K33_RS02275 (C4K33_0458) aroK 505134..505652 (+) 519 WP_009041824.1 shikimate kinase AroK -
  C4K33_RS02280 (C4K33_0459) aroB 505801..506901 (+) 1101 WP_063427911.1 3-dehydroquinate synthase -
  C4K33_RS02285 (C4K33_0460) - 506912..508501 (+) 1590 WP_063427912.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74561.66 Da        Isoelectric Point: 5.1970

>NTDB_id=279694 C4K33_RS02270 WP_063427909.1 503057..505129(+) (pilQ) [Pseudomonas chlororaphis subsp. piscium strain PCL1391]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLANKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAAQSAPGLPAVASGPAKAAASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLAFAKARLPEPLRVRLDVKDFATPVQFVSARAETGKAIITIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNVERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=279694 C4K33_RS02270 WP_063427909.1 503057..505129(+) (pilQ) [Pseudomonas chlororaphis subsp. piscium strain PCL1391]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGCCCGGTGCCCGAGCCGCGGG
GTTATACCACCGACCAGCCGGCGCGGATTGCCCTCGATCTGCCGGGTGTCGTCAGCCAGCTGGCGAACAAGAGCCGCGAT
CTGGGCAATGGCAATGCGCGCAGCGTGACGGTGGTCGAATCGGGGCAGCGTACCCGGGTGGTGGTCAACCTGGTCGCGCT
GGCGCCCTACAGCACCCGGGTCGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCAGAGTGCAC
CGGGGCTGCCTGCTGTCGCGTCCGGGCCGGCCAAGGCCGCGGCTTCCGCGGGGCGGTCGATTCGTAGCGTGGATTTCCAG
CGCGGCGAGCAGGGCGAGGGCAATGTACTGATCGACCTCTCCGATTCGGGAATGAGCCCGGATATACAGGAGCGCGACGG
CAAGCTCGTCCTCGCCTTCGCCAAGGCCCGCCTGCCAGAGCCCTTGCGGGTCAGGCTCGACGTCAAGGATTTCGCCACGC
CGGTGCAGTTCGTCAGTGCCCGGGCGGAGACCGGCAAGGCCATCATCACCATCGAACCTGGCGGCACCTTCGATTACTCG
ACCTACCAGACGGACAACAAGCTTACGGTGAGCGTCAGGCCGATGACCTCGGGCGATCTGCAGAAGCGCAACGTCGAGCG
CTCGGCCTACAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTTCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCTCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCCAGGTGGGTAATGTGTTGCTGGTGGCGCCGGCCGATGA
AATCGCCGCTCGCGAACGCCAGGAGCTGGAGTCACAGAAGCAGATAGCCGACCTGGAGCCGCTGCGGCGCGAACTGCTAC
AGGTCAACTACGCCAAGGCCGCGGAAATCGCCAAGTTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGTCCGATGAGCGT
GGTTCGATCACCGTCGATGAACGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCACGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGAGTCAGCAACGGCACCAACGGC
TCTTCCACCATAGGCACGCCGGGCAGTACCAGCAGCAATTCACCCTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAACTGACGGCCATGGAAAAGACCGGTAACGGTGAAA
TCGTCTCCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGCACGGAAATCCCTTATCAGGAA
GCGGCCTCCAGCGGAGCGACCTCGGTGTCCTTCAAGGAGGCCTCGCTGTCCCTGGAGGTGACGCCGCAAATTACCCCGGA
TAATCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCA
AGAAAAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTTATCGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGACTTTTCCGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

AMIN domain (AMIN)

(40-122)

Secretin and TonB N terminus short domain (STN)

(298-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.097

100

0.73
  comQ Acinetobacter baylyi ADP1

40.11

100

0.423
  pilQ Acinetobacter baumannii strain A118

38.165

100

0.416
  pilQ Acinetobacter baumannii D1279779

38.165

100

0.416
  pilQ Legionella pneumophila strain Lp02

38.131

97.681

0.372
  pilQ Legionella pneumophila strain ERS1305867

38.209

97.101

0.371

Multiple sequence alignment