Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C4K33_RS02250 Genome accession   NZ_CP027736
Coordinates   500286..501338 (+) Length   350 a.a.
NCBI ID   WP_063427902.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. piscium strain PCL1391     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 495286..506338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K33_RS02240 (C4K33_0450) - 496183..497451 (+) 1269 WP_025808196.1 malic enzyme-like NAD(P)-binding protein -
  C4K33_RS02245 (C4K33_0451) - 497634..500069 (-) 2436 WP_164485792.1 penicillin-binding protein 1A -
  C4K33_RS02250 (C4K33_0453) pilM 500286..501338 (+) 1053 WP_063427902.1 pilus assembly protein PilM Machinery gene
  C4K33_RS02255 (C4K33_0454) - 501338..501904 (+) 567 WP_025808191.1 PilN domain-containing protein -
  C4K33_RS02260 (C4K33_0455) pilO 501901..502518 (+) 618 WP_063427904.1 type 4a pilus biogenesis protein PilO -
  C4K33_RS02265 (C4K33_0456) - 502515..503060 (+) 546 WP_063427907.1 pilus assembly protein PilP -
  C4K33_RS02270 (C4K33_0457) pilQ 503057..505129 (+) 2073 WP_063427909.1 type IV pilus secretin PilQ Machinery gene
  C4K33_RS02275 (C4K33_0458) aroK 505134..505652 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37141.58 Da        Isoelectric Point: 5.0043

>NTDB_id=279693 C4K33_RS02250 WP_063427902.1 500286..501338(+) (pilM) [Pseudomonas chlororaphis subsp. piscium strain PCL1391]
MLGLFSKKASSLLGIDISSTSVKLLELSRAGNRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLSDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEM
AGLTARVVDLEACALERSFALAAGSSVARAVALIDIGATLTTLSVVHEGQIIYSREQLFGGYQLTEHIQRRYRLSKEEAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQLFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=279693 C4K33_RS02250 WP_063427902.1 500286..501338(+) (pilM) [Pseudomonas chlororaphis subsp. piscium strain PCL1391]
GTGCTAGGACTCTTCAGCAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGCCGGCAACCGCTTTCGGGTCGAGGCCTATGGGGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCCTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGGGCGAAGCATGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGACTTTCCGATGACGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATTCCCTATCCACTGGACGAGGCGGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAGCGGGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTCGAAATG
GCGGGATTGACGGCGCGGGTGGTGGACCTGGAAGCCTGCGCGCTGGAGCGGTCGTTCGCCCTGGCGGCCGGGTCATCGGT
TGCCCGGGCCGTGGCGCTGATCGATATCGGCGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGCGGGTACCAGTTGACCGAGCACATCCAGCGTCGCTACAGGCTGTCGAAAGAAGAGGCCGGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGACTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTGGTACAGCA
GGTTTCGCGCTCGTTGCAGCTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGATTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGGCTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCCGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATTGGCGGCAAGGTCGATGCCGCGGCCCTGGCCAGCGAGGCTCCGTCCCTGATGATCGCCTGTGGCCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.545

100

0.549
  comM Acinetobacter nosocomialis M2

54.261

100

0.546
  comM Acinetobacter baylyi ADP1

51.705

100

0.52
  pilM Legionella pneumophila strain ERS1305867

45.198

100

0.457

Multiple sequence alignment