Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C4K34_RS26860 Genome accession   NZ_CP027735
Coordinates   6040735..6041952 (+) Length   405 a.a.
NCBI ID   WP_124303621.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. piscium strain DTR133     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6035735..6046952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K34_RS26845 (C4K34_5440) - 6036192..6039107 (+) 2916 WP_173678550.1 DUF748 domain-containing protein -
  C4K34_RS26850 (C4K34_5441) - 6039310..6039666 (-) 357 WP_025807489.1 BON domain-containing protein -
  C4K34_RS26855 (C4K34_5442) - 6039955..6040371 (-) 417 WP_063433049.1 pilin -
  C4K34_RS26860 (C4K34_5443) pilC 6040735..6041952 (+) 1218 WP_124303621.1 type II secretion system F family protein Machinery gene
  C4K34_RS26865 (C4K34_5444) pilD 6041955..6042827 (+) 873 WP_063433051.1 A24 family peptidase Machinery gene
  C4K34_RS26870 (C4K34_5445) coaE 6042824..6043447 (+) 624 WP_063433052.1 dephospho-CoA kinase -
  C4K34_RS26875 (C4K34_5446) yacG 6043444..6043647 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  C4K34_RS26880 (C4K34_5447) - 6043652..6043867 (-) 216 WP_025807496.1 hypothetical protein -
  C4K34_RS26885 (C4K34_5448) - 6043933..6044622 (-) 690 WP_063433053.1 energy-coupling factor ABC transporter permease -
  C4K34_RS26890 (C4K34_5449) - 6044619..6045086 (-) 468 WP_063433054.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44592.39 Da        Isoelectric Point: 10.4288

>NTDB_id=279679 C4K34_RS26860 WP_124303621.1 6040735..6041952(+) (pilC) [Pseudomonas chlororaphis subsp. piscium strain DTR133]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHNPSLIRAQLRKQGISPARVRKKSRSLFQRTPPIRARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLMAELKQDIAAGSSFTTALRKRPRYFDELYCSLVDAGEQAGALETLLDRVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQHWWWLLAGGLLLSVFGLR
HAHRHSPRFRQWLDVGLLKLPLVAPLMYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDSMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=279679 C4K34_RS26860 WP_124303621.1 6040735..6041952(+) (pilC) [Pseudomonas chlororaphis subsp. piscium strain DTR133]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATAACCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAGCCCGGCCAGGGTCAGGAAGAAATCCC
GTTCGCTGTTCCAGCGCACGCCCCCGATCAGGGCCCGCGACATCAGCTTGTTCACCCGGCAGCTGGCGACCCTGATCAAG
GCCGGCGTGCCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGACAACCCGAACATGCGCAAACTGATGGCCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCACAGCATTGCGCAAGAGGCCGCGGTACTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAACAGGCCGGGGCCCTGGAAACCCTGCTGGATCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACGCGCATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTCACGGGCAT
TCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAAGGCTTCGGCGCGCAACTGCCGGTGTTCACCCAACTGG
TCATCGCCCTCTCGGAGTTCGTGCAGCACTGGTGGTGGCTGTTGGCGGGCGGTCTGTTGCTCAGCGTGTTCGGCCTGCGC
CATGCCCACCGGCACTCACCGCGCTTTCGCCAGTGGCTGGACGTGGGCCTGCTGAAACTGCCGTTGGTAGCGCCGCTGAT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCACTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAATCAGGTGTTCAAGCATGCGGTGAACCGCATCAGGCAGGACGTGGCGACTGGCATG
CAGTTGCACTTTTCCATGCGCGCGTCCGGCATCTTTCCCGGCATGGCCATCCAGATGACCGCCATCGGCGAAGAGTCCGG
GGCCCTGGACAGCATGCTGGAAAAGGTCGCGATCCACTATGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAACCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGTACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.432

100

0.654
  pilC Acinetobacter baylyi ADP1

55.882

100

0.563
  pilC Acinetobacter baumannii D1279779

55.147

100

0.556
  pilC Legionella pneumophila strain ERS1305867

53.03

97.778

0.519
  pilG Neisseria gonorrhoeae MS11

41.294

99.259

0.41
  pilG Neisseria meningitidis 44/76-A

40.547

99.259

0.402
  pilC Vibrio cholerae strain A1552

41.327

96.79

0.4
  pilC Vibrio campbellii strain DS40M4

38.06

99.259

0.378

Multiple sequence alignment