Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C4K11_RS25935 Genome accession   NZ_CP027722
Coordinates   5762254..5763471 (+) Length   405 a.a.
NCBI ID   WP_124355434.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aureofaciens strain C50     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5757254..5768471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K11_RS25920 (C4K11_5269) - 5757708..5760626 (+) 2919 WP_190300837.1 DUF748 domain-containing protein -
  C4K11_RS25925 (C4K11_5270) - 5760829..5761185 (-) 357 WP_009050906.1 BON domain-containing protein -
  C4K11_RS25930 (C4K11_5271) - 5761473..5761889 (-) 417 WP_009050907.1 pilin -
  C4K11_RS25935 (C4K11_5272) pilC 5762254..5763471 (+) 1218 WP_124355434.1 type II secretion system F family protein Machinery gene
  C4K11_RS25940 (C4K11_5273) pilD 5763474..5764346 (+) 873 WP_009050909.1 prepilin peptidase Machinery gene
  C4K11_RS25945 (C4K11_5274) coaE 5764343..5764966 (+) 624 WP_009045669.1 dephospho-CoA kinase -
  C4K11_RS25950 (C4K11_5275) yacG 5764963..5765166 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  C4K11_RS25955 (C4K11_5276) - 5765171..5765386 (-) 216 WP_009050911.1 hypothetical protein -
  C4K11_RS25960 (C4K11_5277) - 5765452..5766141 (-) 690 WP_110177023.1 energy-coupling factor ABC transporter permease -
  C4K11_RS25965 (C4K11_5278) - 5766138..5766605 (-) 468 WP_124355435.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44407.18 Da        Isoelectric Point: 10.2685

>NTDB_id=279364 C4K11_RS25935 WP_124355434.1 5762254..5763471(+) (pilC) [Pseudomonas chlororaphis subsp. aureofaciens strain C50]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGISPARVRKKSSSLFQRTPPIKARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLLGELKQDIAAGSSFTSALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVITLSEFVQHWWWLLAGALLLSVFGLC
HAHRRSPRFRHWLDAALLKLPLVAPLLYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=279364 C4K11_RS25935 WP_124355434.1 5762254..5763471(+) (pilC) [Pseudomonas chlororaphis subsp. aureofaciens strain C50]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAGCCCGGCCAGGGTCAGGAAGAAATCCA
GCTCGCTGTTCCAGCGCACGCCCCCGATCAAGGCTCGCGACATCAGCCTGTTCACCCGGCAGTTGGCGACCCTGATCAAG
GCCGGCGTACCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGATAACCCGAACATGCGCAAACTGCTGGGCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCAGCGCACTGCGCAAGAGGCCGCGGTATTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAGCAGGCCGGCGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACCCGAATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTCACGGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAAGGCTTCGGCGCGCAACTGCCGGTGTTCACCCAACTGG
TCATCACCCTCTCGGAATTCGTGCAGCACTGGTGGTGGCTGCTGGCGGGCGCTCTGTTGCTCAGCGTGTTCGGCCTGTGT
CATGCCCACCGGCGCTCACCGCGCTTTCGCCACTGGCTGGACGCGGCCCTGCTGAAACTGCCGTTGGTAGCGCCGCTGCT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCGCTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAACCAGGTGTTCAAGCATGCGGTGAACCGCATCAGGCAGGACGTGGCGACCGGCATG
CAGTTGCACTTTTCCATGCGCGCGTCCGGTATCTTTCCCGGCATGGCGATCCAGATGACCGCCATCGGCGAAGAATCCGG
GGCCCTGGACGGCATGCTGGAAAAAGTCGCGATCCACTACGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAGCCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.926

100

0.659
  pilC Acinetobacter baylyi ADP1

55.882

100

0.563
  pilC Acinetobacter baumannii D1279779

55.147

100

0.556
  pilC Legionella pneumophila strain ERS1305867

53.03

97.778

0.519
  pilG Neisseria gonorrhoeae MS11

42.928

99.506

0.427
  pilG Neisseria meningitidis 44/76-A

42.184

99.506

0.42
  pilC Vibrio cholerae strain A1552

42.347

96.79

0.41
  pilC Vibrio campbellii strain DS40M4

39.552

99.259

0.393

Multiple sequence alignment