Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4K13_RS02270 Genome accession   NZ_CP027720
Coordinates   502950..505022 (+) Length   690 a.a.
NCBI ID   WP_041985502.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aureofaciens strain DSM 6698     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 497950..510022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K13_RS02250 (C4K13_0457) pilM 500180..501232 (+) 1053 WP_009046597.1 pilus assembly protein PilM Machinery gene
  C4K13_RS02255 (C4K13_0458) - 501232..501798 (+) 567 WP_009046598.1 PilN domain-containing protein -
  C4K13_RS02260 (C4K13_0459) pilO 501795..502412 (+) 618 WP_009046599.1 type 4a pilus biogenesis protein PilO -
  C4K13_RS02265 (C4K13_0460) - 502409..502936 (+) 528 WP_041985501.1 pilus assembly protein PilP -
  C4K13_RS02270 (C4K13_0461) pilQ 502950..505022 (+) 2073 WP_041985502.1 type IV pilus secretin PilQ Machinery gene
  C4K13_RS02275 (C4K13_0462) aroK 505027..505545 (+) 519 WP_009041824.1 shikimate kinase AroK -
  C4K13_RS02280 (C4K13_0463) aroB 505693..506793 (+) 1101 WP_016704447.1 3-dehydroquinate synthase -
  C4K13_RS02285 (C4K13_0464) - 506804..508393 (+) 1590 WP_016704448.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74642.78 Da        Isoelectric Point: 5.4337

>NTDB_id=279301 C4K13_RS02270 WP_041985502.1 502950..505022(+) (pilQ) [Pseudomonas chlororaphis subsp. aureofaciens strain DSM 6698]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLANKSRD
LGSGNARSVTVVESGPRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATVAQSAPGQPAVASGRAKATASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNA
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=279301 C4K13_RS02270 WP_041985502.1 502950..505022(+) (pilQ) [Pseudomonas chlororaphis subsp. aureofaciens strain DSM 6698]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCGCTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGACCGGTGCCCGAGCCGCGGG
GTTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAGCTGGCGAACAAGAGCCGCGAT
CTGGGCAGTGGCAATGCGCGCAGCGTGACGGTGGTCGAATCGGGGCCGCGTACCCGGGTGGTGGTCAATCTGGTCGCGCT
GGCGCCCTACAGCACTCGAGTCGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGTGGCGCAGAGTGCAC
CGGGGCAGCCGGCTGTCGCGTCCGGGCGGGCCAAGGCCACGGCTTCGGCGGGGCGGTCGATTCGCAGTGTGGATTTCCAG
CGCGGCGAGCAGGGTGAAGGCAATGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATCCAGGAACGCGACGG
CAAGCTCGTCCTTACCTTCGCCAAGGCCCGTCTGCCAGAACCCCTGCGGGTCAGGCTGGACGTCAAGGACTTCGCCACTC
CCGTGCAATTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACCTTCGATTACTCG
ACCTACCAGACGGACAACAAACTCACGGTAAGTGTCAGGCCGATGACTTCGGGCGATCTGCAGAAACGCAACGCCGAGCG
CTCGGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAAAATGTGCCGTGGGACCAG
GCTCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAATGTGTTGCTGGTGGCGCCAGCCGATGA
AATCGCGGCCCGCGAACGCCAGGAGCTGGAGTCGCAGAAGCAGATCGCTGATCTCGAGCCGCTGCGGCGTGAACTGCTGC
AGGTCAACTATGCCAAGGCGGCGGAGATCGCCAAGCTGTTCCAGTCGGTCACCCGCGCTGAGGACAAGTCTGACGAGCGT
GGTTCGATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGTGGCTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGCC
TCTTCCACCATAGGCACGCCAGGCAGTACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAGCTGACAGCCATGGAAAAGACCGGTAACGGCGAAA
TCGTCTCCCAGCCCAAGGTCGTCACCTCCGACAAGGAGACGGCGAAGATCCTCAAGGGCACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGTGGGGCGACTTCGGTGTCGTTCAAGGAGGCGTCGCTGTCCCTGGAGGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTTATCGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTAGGCGATGTGCCGTATCTTGGCCGCCTTTTCAGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(298-344)

AMIN domain (AMIN)

(40-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.909

100

0.735
  comQ Acinetobacter baylyi ADP1

39.617

100

0.42
  pilQ Acinetobacter baumannii strain A118

37.55

100

0.404
  pilQ Acinetobacter baumannii D1279779

37.55

100

0.404
  pilQ Legionella pneumophila strain Lp02

38.039

97.536

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.117

96.957

0.37

Multiple sequence alignment