Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C4K13_RS02250 Genome accession   NZ_CP027720
Coordinates   500180..501232 (+) Length   350 a.a.
NCBI ID   WP_009046597.1    Uniprot ID   A0AB33WJJ4
Organism   Pseudomonas chlororaphis subsp. aureofaciens strain DSM 6698     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 495180..506232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K13_RS02240 (C4K13_0454) - 496132..497400 (+) 1269 WP_009046595.1 malic enzyme-like NAD(P)-binding protein -
  C4K13_RS02245 (C4K13_0455) - 497528..499963 (-) 2436 WP_164485209.1 penicillin-binding protein 1A -
  C4K13_RS02250 (C4K13_0457) pilM 500180..501232 (+) 1053 WP_009046597.1 pilus assembly protein PilM Machinery gene
  C4K13_RS02255 (C4K13_0458) - 501232..501798 (+) 567 WP_009046598.1 PilN domain-containing protein -
  C4K13_RS02260 (C4K13_0459) pilO 501795..502412 (+) 618 WP_009046599.1 type 4a pilus biogenesis protein PilO -
  C4K13_RS02265 (C4K13_0460) - 502409..502936 (+) 528 WP_041985501.1 pilus assembly protein PilP -
  C4K13_RS02270 (C4K13_0461) pilQ 502950..505022 (+) 2073 WP_041985502.1 type IV pilus secretin PilQ Machinery gene
  C4K13_RS02275 (C4K13_0462) aroK 505027..505545 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37047.51 Da        Isoelectric Point: 5.0008

>NTDB_id=279300 C4K13_RS02250 WP_009046597.1 500180..501232(+) (pilM) [Pseudomonas chlororaphis subsp. aureofaciens strain DSM 6698]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGASVARTVALVDIGATLTALSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSKEEAS
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=279300 C4K13_RS02250 WP_009046597.1 500180..501232(+) (pilM) [Pseudomonas chlororaphis subsp. aureofaciens strain DSM 6698]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTTATCGAGATGGAGGCCGGGCTTTGCGATGACGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGATGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCTTGTCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAGGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCGCCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGAGGCCAGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTGGTGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCTATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGACTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCTGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.398

100

0.557
  comM Acinetobacter nosocomialis M2

55.114

100

0.554
  comM Acinetobacter baylyi ADP1

51.989

100

0.523
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.46

Multiple sequence alignment