Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4K25_RS02220 Genome accession   NZ_CP027714
Coordinates   496059..498131 (+) Length   690 a.a.
NCBI ID   WP_114760244.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain ATCC 17415     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 491059..503131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K25_RS02200 (C4K25_0442) pilM 493289..494341 (+) 1053 WP_037004437.1 pilus assembly protein PilM Machinery gene
  C4K25_RS02205 (C4K25_0443) - 494341..494907 (+) 567 WP_114760241.1 PilN domain-containing protein -
  C4K25_RS02210 (C4K25_0444) pilO 494904..495521 (+) 618 WP_114760242.1 type 4a pilus biogenesis protein PilO -
  C4K25_RS02215 (C4K25_0445) - 495518..496045 (+) 528 WP_114760243.1 pilus assembly protein PilP -
  C4K25_RS02220 (C4K25_0446) pilQ 496059..498131 (+) 2073 WP_114760244.1 type IV pilus secretin PilQ Machinery gene
  C4K25_RS02225 (C4K25_0447) aroK 498136..498654 (+) 519 WP_114760245.1 shikimate kinase AroK -
  C4K25_RS02230 (C4K25_0448) aroB 498803..499903 (+) 1101 WP_016704447.1 3-dehydroquinate synthase -
  C4K25_RS02235 (C4K25_0449) - 499914..501503 (+) 1590 WP_114760246.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74737.88 Da        Isoelectric Point: 5.2079

>NTDB_id=279143 C4K25_RS02220 WP_114760244.1 496059..498131(+) (pilQ) [Pseudomonas chlororaphis strain ATCC 17415]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLADKSRD
LGNGNARSVTVVESGQRTRVVVNLIALAPYSTRVAGNQLFVVIGQGATAALSAPGQPAVASGRAKAVASVGRSIHSVDFQ
RGEQGEGNVLIDLSDSEMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=279143 C4K25_RS02220 WP_114760244.1 496059..498131(+) (pilQ) [Pseudomonas chlororaphis strain ATCC 17415]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGTTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAGCTGACCTTCGATGGGCCGGTGCCCGAGCCGCGCG
GCTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCCGGTGTCGTCAGCCAGCTGGCAGACAAGAGCCGTGAC
CTGGGCAATGGCAATGCGCGCAGTGTGACGGTGGTCGAATCGGGGCAGCGTACCCGGGTTGTCGTCAATCTGATCGCGCT
GGCGCCCTACAGCACTCGGGTCGCTGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCTGAGTGCGC
CGGGGCAACCGGCTGTCGCGTCGGGGCGGGCCAAGGCCGTGGCTTCCGTGGGACGGTCGATTCACAGCGTGGATTTCCAG
CGCGGCGAGCAGGGCGAGGGGAATGTACTGATCGACCTCTCCGACTCGGAAATGAGCCCGGACATACAGGAACGCGACGG
CAAACTCGTCCTCACCTTCGCCAAGGCCCGCCTGCCCGAGCCCCTGCGGGTCAGGCTGGACGTCAAGGATTTCGCCACCC
CCGTGCAGTTCGTCAGCGCCCGGGCAGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACTTTCGATTACTCG
ACCTACCAGACGGACAACAAACTCACGGTGAGCGTCAGGCCGATGACCTCTGGCGACCTGCAGAAACGCAACGCCGAGCG
CTCGGCCTACAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGATT
TCACCAACCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAATGTGTTGCTGGTGGCGCCGGCCGATGA
AATTGCTGCCCGCGAACGCCAGGAACTGGAATCGCAGAAGCAGATCGCCGACCTGGAACCATTGCGGCGCGAACTGCTGC
AGGTCAACTATGCCAAGGCCGCGGAGATCGCCAAGTTGTTCCAGTCGGTCACCCGCGCTGAGGACAAGTCCGACGAACGC
GGGTCGATCACTGTCGATGAGCGGACCAACAACATCATCGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
CGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAATCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAATGGT
TCCTCCACCATAGGCACGCCGGGCAGCACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACTACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAATGTGTTGCTGGATCTTGAGCTGACAGCCATGGAAAAGACCGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGGACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCGACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCCCTGGAGGTGACGCCACAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAAGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGATGGAGAGACCATCGTCATCGGTGGAGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

AMIN domain (AMIN)

(40-122)

Secretin and TonB N terminus short domain (STN)

(298-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.629

100

0.732
  comQ Acinetobacter baylyi ADP1

40.055

100

0.423
  pilQ Acinetobacter baumannii strain A118

38.121

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.121

100

0.412
  pilQ Legionella pneumophila strain Lp02

38.039

97.536

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.117

96.957

0.37

Multiple sequence alignment