Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C4K25_RS02200 Genome accession   NZ_CP027714
Coordinates   493289..494341 (+) Length   350 a.a.
NCBI ID   WP_037004437.1    Uniprot ID   A0AAX2E8Q7
Organism   Pseudomonas chlororaphis strain ATCC 17415     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 488289..499341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K25_RS02190 (C4K25_0439) - 489227..490495 (+) 1269 WP_062823907.1 malic enzyme-like NAD(P)-binding protein -
  C4K25_RS02195 (C4K25_0440) - 490637..493072 (-) 2436 WP_164485849.1 penicillin-binding protein 1A -
  C4K25_RS02200 (C4K25_0442) pilM 493289..494341 (+) 1053 WP_037004437.1 pilus assembly protein PilM Machinery gene
  C4K25_RS02205 (C4K25_0443) - 494341..494907 (+) 567 WP_114760241.1 PilN domain-containing protein -
  C4K25_RS02210 (C4K25_0444) pilO 494904..495521 (+) 618 WP_114760242.1 type 4a pilus biogenesis protein PilO -
  C4K25_RS02215 (C4K25_0445) - 495518..496045 (+) 528 WP_114760243.1 pilus assembly protein PilP -
  C4K25_RS02220 (C4K25_0446) pilQ 496059..498131 (+) 2073 WP_114760244.1 type IV pilus secretin PilQ Machinery gene
  C4K25_RS02225 (C4K25_0447) aroK 498136..498654 (+) 519 WP_114760245.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37125.48 Da        Isoelectric Point: 4.9975

>NTDB_id=279142 C4K25_RS02200 WP_037004437.1 493289..494341(+) (pilM) [Pseudomonas chlororaphis strain ATCC 17415]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLSDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGSPTARTVALVDIGATLTTLSVVHEGQIVYSREQLFGGHQLTEHIQRRYGLSKEEAG
QAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASAPQHQVDSILLAGGSASVPGLVPLIEQRLGLPALIANPFVGM
SIGARVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=279142 C4K25_RS02200 WP_037004437.1 493289..494341(+) (pilM) [Pseudomonas chlororaphis strain ATCC 17415]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAATT
GAGCCGTGTCGGTGGCCGCTTTCGGGTCGAGGCCTATGGCGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCTGAAGGGGTCGGGCATGCCCTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGTGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTGTCCGACGACGATCGGGAAAGCCAGCT
CAAGGTCGACGCCGACCAGTACATTCCCTACCCGCTGGACGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCCC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAGCACATCGAGGTGCGCGAGGCGGCGCTCGAACTT
GCGGGCCTGACTGCGCGAGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGTTCGTTCGCCCTGGCGGCCGGCTCGCCGAC
TGCCCGGACCGTGGCGCTGGTCGATATCGGCGCCACCCTGACCACCCTGAGCGTCGTGCATGAAGGGCAGATCGTCTATT
CCCGTGAGCAGTTGTTCGGCGGACACCAACTGACCGAACACATCCAGCGCCGCTACGGGCTGTCGAAAGAAGAGGCCGGC
CAGGCAAAAAAGCAGGGTGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAACA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGCCCCGCAACACCAGGTCGATTCCATTCTGTTGGCGGGTGGCAGCG
CCTCGGTTCCGGGGCTTGTCCCGTTGATCGAGCAGCGCCTGGGCTTGCCTGCGCTGATCGCCAACCCTTTCGTTGGCATG
AGCATCGGCGCCAGGGTCGACGCCGCGGCCCTGGCCAGCGAGGCCCCATCCTTGATGATCGCCTGCGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.398

100

0.557
  comM Acinetobacter nosocomialis M2

55.114

100

0.554
  comM Acinetobacter baylyi ADP1

52.273

100

0.526
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.454

Multiple sequence alignment