Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4K26_RS02385 Genome accession   NZ_CP027713
Coordinates   531326..533398 (+) Length   690 a.a.
NCBI ID   WP_124349207.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain TAMOak81     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 526326..538398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K26_RS02365 (C4K26_0475) pilM 528556..529608 (+) 1053 WP_124349203.1 pilus assembly protein PilM Machinery gene
  C4K26_RS02370 (C4K26_0476) - 529608..530174 (+) 567 WP_124349204.1 PilN domain-containing protein -
  C4K26_RS02375 (C4K26_0477) pilO 530171..530788 (+) 618 WP_124349205.1 type 4a pilus biogenesis protein PilO -
  C4K26_RS02380 (C4K26_0478) - 530785..531312 (+) 528 WP_124349206.1 pilus assembly protein PilP -
  C4K26_RS02385 (C4K26_0479) pilQ 531326..533398 (+) 2073 WP_124349207.1 type IV pilus secretin PilQ Machinery gene
  C4K26_RS02390 (C4K26_0480) aroK 533403..533921 (+) 519 WP_009041824.1 shikimate kinase AroK -
  C4K26_RS02395 (C4K26_0481) aroB 534070..535170 (+) 1101 WP_087089784.1 3-dehydroquinate synthase -
  C4K26_RS02400 (C4K26_0482) - 535181..536770 (+) 1590 WP_124349208.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74728.91 Da        Isoelectric Point: 5.3081

>NTDB_id=279117 C4K26_RS02385 WP_124349207.1 531326..533398(+) (pilQ) [Pseudomonas chlororaphis strain TAMOak81]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLADKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAALSAPGQPAVVSGRAKAVASVGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNAERSTYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=279117 C4K26_RS02385 WP_124349207.1 531326..533398(+) (pilQ) [Pseudomonas chlororaphis strain TAMOak81]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGTTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAGCTGACCTTCGATGGACCGGTGCCCGAGCCGCGCG
GCTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCCGGTGTTGTCAGCCAGCTGGCAGACAAGAGCCGTGAC
CTGGGCAATGGCAATGCGCGCAGCGTGACGGTGGTCGAATCGGGGCAGCGTACCCGGGTCGTCGTCAATCTGGTCGCGCT
GGCGCCCTACAGCACTCGGGTCGCTGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCTGAGTGCGC
CGGGGCAGCCGGCTGTCGTGTCCGGGCGGGCCAAGGCCGTGGCTTCCGTGGGCCGGTCGATTCGCAGCGTGGATTTCCAG
CGTGGCGAGCAGGGCGAGGGGAATGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATCCAGGAACGCGACGG
CAAGCTCGTCCTGACCTTTGCCAAGGCCCGCCTGCCCGAGCCCCTGCGGGTCAGGCTGGACGTCAAGGATTTCGCCACGC
CGGTGCAGTTTGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACTTTCGATTACTCG
ACCTACCAGACGGACAACAAACTCACGGTGAGCGTCAGACCGATGACCTCGGGCGATCTGCAGAAACGCAACGCCGAGCG
CTCGACCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATTGAGGTCCGCTCGGTGCTGCAACTGATCGCCGATT
TCACCAATCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAACGTGCCGTGGGACCAG
GCCCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTAGGCAACGTGTTGCTGGTGGCGCCGGCCGATGA
AATCGCTGCCCGCGAACGTCAGGAACTGGAGTCGCAGAAGCAGATCGCCGACCTGGAACCATTGCGGCGCGAACTGCTGC
AGGTCAACTACGCCAAGGCCGCGGAGATCGCCAAGTTGTTCCAATCGGTCACCCGCGCCGAGGACAAGTCCGACGAACGC
GGGTCGATCACTGTCGATGAGCGGACCAACAACATCATCGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
CGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGT
TCCTCCACCATAGGCACGCCGGGCAGTACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACTACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAATGTGTTGCTGGACCTTGAGCTGACAGCCATGGAAAAGACCGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTGGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGTACGGAGATCCCTTATCAGGAA
GCCGCCTCCAGTGGGGCGACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCCCTGGAGGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAAGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGATGGAGAGACCATCGTCATCGGTGGAGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(298-344)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

AMIN domain (AMIN)

(40-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.49

100

0.73
  comQ Acinetobacter baylyi ADP1

40.055

100

0.423
  pilQ Acinetobacter baumannii strain A118

38.255

100

0.413
  pilQ Acinetobacter baumannii D1279779

38.255

100

0.413
  pilQ Legionella pneumophila strain Lp02

38.187

97.536

0.372
  pilQ Legionella pneumophila strain ERS1305867

38.266

96.957

0.371

Multiple sequence alignment