Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C4K26_RS02365 Genome accession   NZ_CP027713
Coordinates   528556..529608 (+) Length   350 a.a.
NCBI ID   WP_124349203.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain TAMOak81     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 523556..534608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K26_RS02355 (C4K26_0472) - 524508..525776 (+) 1269 WP_007925851.1 malic enzyme-like NAD(P)-binding protein -
  C4K26_RS02360 (C4K26_0473) - 525904..528339 (-) 2436 WP_164486216.1 penicillin-binding protein 1A -
  C4K26_RS02365 (C4K26_0475) pilM 528556..529608 (+) 1053 WP_124349203.1 pilus assembly protein PilM Machinery gene
  C4K26_RS02370 (C4K26_0476) - 529608..530174 (+) 567 WP_124349204.1 PilN domain-containing protein -
  C4K26_RS02375 (C4K26_0477) pilO 530171..530788 (+) 618 WP_124349205.1 type 4a pilus biogenesis protein PilO -
  C4K26_RS02380 (C4K26_0478) - 530785..531312 (+) 528 WP_124349206.1 pilus assembly protein PilP -
  C4K26_RS02385 (C4K26_0479) pilQ 531326..533398 (+) 2073 WP_124349207.1 type IV pilus secretin PilQ Machinery gene
  C4K26_RS02390 (C4K26_0480) aroK 533403..533921 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37116.51 Da        Isoelectric Point: 4.8999

>NTDB_id=279116 C4K26_RS02365 WP_124349203.1 528556..529608(+) (pilM) [Pseudomonas chlororaphis strain TAMOak81]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLSDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGSPAARTVALVDIGATLTTLSVVHEGQIVYSREQLFGGHQLTEHIQRRYGLSKEEAG
QAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASAPQRQVDSILLAGGSASVPGLVPLIEQRLGLPVLIANPFAGM
SIGAKVDAAALASEAPSLMIACGLALWSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=279116 C4K26_RS02365 WP_124349203.1 528556..529608(+) (pilM) [Pseudomonas chlororaphis strain TAMOak81]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAATT
GAGCCGTGTCGGTGGCCGCTTTCGGGTCGAGGCCTATGGCGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCTGAAGGGGTCGGACATGCCCTGTCTCGCCTGCTGGCCAGGGCCCGCCCGGGTGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTATCCGACGACGATCGGGAAAGCCAGCT
CAAGGTCGACGCCGACCAGTACATTCCCTATCCGCTGGACGAGGCCGCCATCGATTTCGATGTCCTCGGCTATTCGGCCC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAGCACATCGAAGTTCGCGAGGCGGCGCTCGAACTC
GCCGGGCTGACGGCCCGAGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGTTCGTTCGCCCTGGCGGCCGGCTCGCCGGC
TGCCCGGACCGTGGCGCTGGTCGATATCGGCGCCACCCTGACCACCCTGAGCGTCGTGCATGAAGGGCAGATCGTCTATT
CCCGTGAGCAGTTGTTCGGCGGACACCAACTGACCGAACACATCCAGCGTCGCTACGGGCTGTCGAAAGAAGAGGCCGGC
CAGGCAAAAAAGCAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAACA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGCCCCGCAACGCCAGGTCGATTCCATCCTGTTGGCGGGTGGCAGCG
CCTCGGTTCCGGGGCTTGTCCCGTTGATCGAGCAGCGCCTGGGCTTGCCTGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGCCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCCCCGTCCTTGATGATCGCCTGCGGGCTGGCCTTGTG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.114

100

0.554
  comM Acinetobacter nosocomialis M2

54.83

100

0.551
  comM Acinetobacter baylyi ADP1

51.989

100

0.523
  pilM Legionella pneumophila strain ERS1305867

45.714

100

0.457

Multiple sequence alignment