Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4K27_RS02250 Genome accession   NZ_CP027712
Coordinates   504607..506679 (+) Length   690 a.a.
NCBI ID   WP_053259360.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. chlororaphis strain DSM 50083     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 499607..511679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K27_RS02230 (C4K27_0453) pilM 501837..502889 (+) 1053 WP_053259356.1 pilus assembly protein PilM Machinery gene
  C4K27_RS02235 (C4K27_0454) - 502889..503455 (+) 567 WP_053259357.1 PilN domain-containing protein -
  C4K27_RS02240 (C4K27_0455) - 503452..504069 (+) 618 WP_053259358.1 type 4a pilus biogenesis protein PilO -
  C4K27_RS02245 (C4K27_0456) - 504066..504593 (+) 528 WP_053259359.1 pilus assembly protein PilP -
  C4K27_RS02250 (C4K27_0457) pilQ 504607..506679 (+) 2073 WP_053259360.1 type IV pilus secretin PilQ Machinery gene
  C4K27_RS02255 (C4K27_0458) aroK 506684..507202 (+) 519 WP_009041824.1 shikimate kinase AroK -
  C4K27_RS02260 (C4K27_0459) aroB 507351..508451 (+) 1101 WP_053259361.1 3-dehydroquinate synthase -
  C4K27_RS02265 (C4K27_0460) - 508462..510048 (+) 1587 WP_053259362.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74640.85 Da        Isoelectric Point: 5.4337

>NTDB_id=279092 C4K27_RS02250 WP_053259360.1 504607..506679(+) (pilQ) [Pseudomonas chlororaphis subsp. chlororaphis strain DSM 50083]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLADKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAALSAPGQPVVASGRAKAVASVGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERGGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=279092 C4K27_RS02250 WP_053259360.1 504607..506679(+) (pilQ) [Pseudomonas chlororaphis subsp. chlororaphis strain DSM 50083]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGGCCGGTGCCCGAGCCGCGGG
GGTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCCGGTGTTGTCAGCCAGCTGGCAGACAAGAGCCGTGAC
CTGGGCAATGGCAATGCGCGCAGCGTGACGGTGGTCGAATCGGGGCAGCGTACCCGGGTCGTCGTCAATCTGGTCGCGCT
GGCGCCCTACAGCACTCGGGTCGCTGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCTGAGTGCGC
CAGGGCAGCCGGTTGTCGCGTCCGGGCGGGCCAAGGCCGTGGCTTCCGTGGGGCGGTCGATTCGCAGCGTGGATTTCCAG
CGCGGCGAGCAAGGCGAAGGTAATGTGCTGATCGATCTCTCCGACTCGGGAATGAGCCCGGATATACAGGAGCGCGGCGG
CAAGCTCGTCCTCACCTTCGCCAAAGCCCGCCTGCCCGAGCCCCTGCGGGTCAGGCTGGACGTCAAGGATTTCGCCACCC
CCGTGCAGTTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACTTTCGATTACTCG
ACCTACCAGACGGACAACAAACTCACGGTGAGCGTCAGGCCGATGACCTCGGGCGACCTGCAGAAACGCAACGCCGAGCG
CTCGGCCTACAACGGCGAAAAACTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAATTGATCGCCGATT
TCACCAACCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAACGTGTTGCTGGTGGCGCCGGCCGATGA
AATCGCTGCCCGCGAACGCCAGGAACTGGAGTCGCAGAAGCAGATCGCCGACCTGGAACCATTGCGGCGCGAACTGCTGC
AGGTCAACTACGCCAAGGCCGCGGAGATCGCCAAGCTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGTCCGACGAACGC
GGGTCGATCACTGTCGATGAGCGGACCAACAACATCATCGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
CGTCAGCCAACTGGATATCCCGGTGCGCCAGGTGATGATCGAAGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGCGGCTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGT
TCCTCCACCATAGGCACGCCGGGCAGCACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACTACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAATGTGTTGCTGGACCTTGAGCTGACAGCCATGGAAAAGACCGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGCACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCGACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCCCTGGAGGTGACGCCACAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAAGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGATGGAGAGACCATCGTCATCGGTGGAGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAAGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

AMIN domain (AMIN)

(40-122)

Secretin and TonB N terminus short domain (STN)

(298-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.769

100

0.733
  comQ Acinetobacter baylyi ADP1

39.973

100

0.422
  pilQ Acinetobacter baumannii D1279779

38.255

100

0.413
  pilQ Acinetobacter baumannii strain A118

38.255

100

0.413
  pilQ Legionella pneumophila strain Lp02

38.484

97.536

0.375
  pilQ Legionella pneumophila strain ERS1305867

38.565

96.957

0.374

Multiple sequence alignment