Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C4K27_RS02230 Genome accession   NZ_CP027712
Coordinates   501837..502889 (+) Length   350 a.a.
NCBI ID   WP_053259356.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. chlororaphis strain DSM 50083     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 496837..507889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K27_RS02220 (C4K27_0450) - 497789..499057 (+) 1269 WP_007925851.1 malic enzyme-like NAD(P)-binding protein -
  C4K27_RS02225 (C4K27_0451) - 499185..501620 (-) 2436 WP_164523775.1 penicillin-binding protein 1A -
  C4K27_RS02230 (C4K27_0453) pilM 501837..502889 (+) 1053 WP_053259356.1 pilus assembly protein PilM Machinery gene
  C4K27_RS02235 (C4K27_0454) - 502889..503455 (+) 567 WP_053259357.1 PilN domain-containing protein -
  C4K27_RS02240 (C4K27_0455) - 503452..504069 (+) 618 WP_053259358.1 type 4a pilus biogenesis protein PilO -
  C4K27_RS02245 (C4K27_0456) - 504066..504593 (+) 528 WP_053259359.1 pilus assembly protein PilP -
  C4K27_RS02250 (C4K27_0457) pilQ 504607..506679 (+) 2073 WP_053259360.1 type IV pilus secretin PilQ Machinery gene
  C4K27_RS02255 (C4K27_0458) aroK 506684..507202 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37094.50 Da        Isoelectric Point: 4.9018

>NTDB_id=279091 C4K27_RS02230 WP_053259356.1 501837..502889(+) (pilM) [Pseudomonas chlororaphis subsp. chlororaphis strain DSM 50083]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLSDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGSPAARTVALVDIGATLTTLSVVHEGQIVYSREQLFGGHQLTEHIQRRYGLSKEEAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVLRSLQFFQASAPQHQVDSILLAGGSASVPGLVPLIDQRLGLPALIANPFADM
SIGAKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=279091 C4K27_RS02230 WP_053259356.1 501837..502889(+) (pilM) [Pseudomonas chlororaphis subsp. chlororaphis strain DSM 50083]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAATT
GAGCCGTGTCGGTGGCCGTTTTCGGGTCGAGGCCTATGGCGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TAGTCGAGCCTGAAGGGGTCGGGCATGCCCTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGTGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTGTCCGACGACGATCGGGAAAGCCAGCT
CAAGGTCGACGCCGACCAGTACATTCCCTATCCGCTGGACGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCTC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAGCACATCGAAGTTCGCGAGGCGGCGCTCGAGCTC
GCCGGGCTGACGGCCCGAGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGGTCGTTCGCCCTGGCGGCCGGCTCGCCGGC
TGCCCGGACCGTGGCGCTGGTCGATATCGGCGCCACCCTGACCACCCTGAGCGTCGTGCATGAAGGGCAGATCGTCTATT
CCCGTGAGCAGTTGTTCGGCGGGCACCAACTGACCGAACACATCCAGCGTCGCTACGGGCTGTCGAAAGAAGAGGCCGGC
CTGGCGAAAAAGCAGGGTGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCTTTTCAGGAGGCGGTTGTGCAACA
GGTTTTGCGCTCGTTGCAGTTTTTCCAGGCCTCCGCCCCGCAACACCAGGTCGATTCCATCCTGTTAGCGGGTGGCAGCG
CCTCGGTTCCGGGGCTTGTCCCGTTGATCGATCAGCGCCTGGGCTTGCCTGCGCTGATCGCCAACCCCTTCGCTGACATG
AGCATCGGCGCCAAGGTCGACGCCGCAGCCCTGGCCAGCGAAGCCCCGTCCTTGATGATCGCCTGCGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.114

100

0.554
  comM Acinetobacter nosocomialis M2

54.83

100

0.551
  comM Acinetobacter baylyi ADP1

51.989

100

0.523
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.46

Multiple sequence alignment