Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C4K35_RS28270 Genome accession   NZ_CP027710
Coordinates   6227719..6228936 (+) Length   405 a.a.
NCBI ID   WP_124303621.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. piscium strain SLPH10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6222719..6233936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K35_RS28255 (C4K35_5725) - 6223176..6226091 (+) 2916 WP_173678550.1 DUF748 domain-containing protein -
  C4K35_RS28260 (C4K35_5726) - 6226294..6226650 (-) 357 WP_025807489.1 BON domain-containing protein -
  C4K35_RS28265 (C4K35_5727) - 6226939..6227355 (-) 417 WP_063433049.1 pilin -
  C4K35_RS28270 (C4K35_5728) pilC 6227719..6228936 (+) 1218 WP_124303621.1 type II secretion system F family protein Machinery gene
  C4K35_RS28275 (C4K35_5729) pilD 6228939..6229811 (+) 873 WP_063433051.1 prepilin peptidase Machinery gene
  C4K35_RS28280 (C4K35_5730) coaE 6229808..6230431 (+) 624 WP_063433052.1 dephospho-CoA kinase -
  C4K35_RS28285 (C4K35_5731) yacG 6230428..6230631 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  C4K35_RS28290 (C4K35_5732) - 6230636..6230851 (-) 216 WP_025807496.1 hypothetical protein -
  C4K35_RS28295 (C4K35_5733) - 6230917..6231606 (-) 690 WP_063433053.1 energy-coupling factor ABC transporter permease -
  C4K35_RS28300 (C4K35_5734) - 6231603..6232070 (-) 468 WP_063433054.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44592.39 Da        Isoelectric Point: 10.4288

>NTDB_id=279051 C4K35_RS28270 WP_124303621.1 6227719..6228936(+) (pilC) [Pseudomonas chlororaphis subsp. piscium strain SLPH10]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHNPSLIRAQLRKQGISPARVRKKSRSLFQRTPPIRARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLMAELKQDIAAGSSFTTALRKRPRYFDELYCSLVDAGEQAGALETLLDRVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQHWWWLLAGGLLLSVFGLR
HAHRHSPRFRQWLDVGLLKLPLVAPLMYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDSMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=279051 C4K35_RS28270 WP_124303621.1 6227719..6228936(+) (pilC) [Pseudomonas chlororaphis subsp. piscium strain SLPH10]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATAACCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAGCCCGGCCAGGGTCAGGAAGAAATCCC
GTTCGCTGTTCCAGCGCACGCCCCCGATCAGGGCCCGCGACATCAGCTTGTTCACCCGGCAGCTGGCGACCCTGATCAAG
GCCGGCGTGCCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGACAACCCGAACATGCGCAAACTGATGGCCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCACAGCATTGCGCAAGAGGCCGCGGTACTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAACAGGCCGGGGCCCTGGAAACCCTGCTGGATCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACGCGCATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTCACGGGCAT
TCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAAGGCTTCGGCGCGCAACTGCCGGTGTTCACCCAACTGG
TCATCGCCCTCTCGGAGTTCGTGCAGCACTGGTGGTGGCTGTTGGCGGGCGGTCTGTTGCTCAGCGTGTTCGGCCTGCGC
CATGCCCACCGGCACTCACCGCGCTTTCGCCAGTGGCTGGACGTGGGCCTGCTGAAACTGCCGTTGGTAGCGCCGCTGAT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCACTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAATCAGGTGTTCAAGCATGCGGTGAACCGCATCAGGCAGGACGTGGCGACTGGCATG
CAGTTGCACTTTTCCATGCGCGCGTCCGGCATCTTTCCCGGCATGGCCATCCAGATGACCGCCATCGGCGAAGAGTCCGG
GGCCCTGGACAGCATGCTGGAAAAGGTCGCGATCCACTATGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAACCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGTACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.432

100

0.654
  pilC Acinetobacter baylyi ADP1

55.882

100

0.563
  pilC Acinetobacter baumannii D1279779

55.147

100

0.556
  pilC Legionella pneumophila strain ERS1305867

53.03

97.778

0.519
  pilG Neisseria gonorrhoeae MS11

41.294

99.259

0.41
  pilG Neisseria meningitidis 44/76-A

40.547

99.259

0.402
  pilC Vibrio cholerae strain A1552

41.327

96.79

0.4
  pilC Vibrio campbellii strain DS40M4

38.06

99.259

0.378

Multiple sequence alignment