Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4K38_RS02330 Genome accession   NZ_CP027707
Coordinates   512796..514868 (+) Length   690 a.a.
NCBI ID   WP_053277123.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. piscium strain DSM 21509     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 507796..519868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K38_RS02310 (C4K38_0463) pilM 510026..511078 (+) 1053 WP_053277119.1 pilus assembly protein PilM Machinery gene
  C4K38_RS02315 (C4K38_0464) - 511078..511644 (+) 567 WP_053277120.1 PilN domain-containing protein -
  C4K38_RS02320 (C4K38_0465) pilO 511641..512258 (+) 618 WP_053277121.1 type 4a pilus biogenesis protein PilO -
  C4K38_RS02325 (C4K38_0466) - 512255..512782 (+) 528 WP_053277122.1 pilus assembly protein PilP -
  C4K38_RS02330 (C4K38_0467) pilQ 512796..514868 (+) 2073 WP_053277123.1 type IV pilus secretin PilQ Machinery gene
  C4K38_RS02335 (C4K38_0468) aroK 514873..515391 (+) 519 WP_009041824.1 shikimate kinase AroK -
  C4K38_RS02340 (C4K38_0469) aroB 515540..516640 (+) 1101 WP_053277124.1 3-dehydroquinate synthase -
  C4K38_RS02345 (C4K38_0470) - 516651..518240 (+) 1590 WP_053277125.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74661.82 Da        Isoelectric Point: 5.3013

>NTDB_id=278959 C4K38_RS02330 WP_053277123.1 512796..514868(+) (pilQ) [Pseudomonas chlororaphis subsp. piscium strain DSM 21509]
MNRIFSAFGIALWMALLSPMVMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVASQLVSKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVVGNQLFVVIGQGATAAQSAPGLPDVAPGRAKAAASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIITIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGATANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=278959 C4K38_RS02330 WP_053277123.1 512796..514868(+) (pilQ) [Pseudomonas chlororaphis subsp. piscium strain DSM 21509]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGTTGCTTTCACCGATGGTCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGGCCGGTGCCCGAACCGCGGG
GTTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGCCAGCCAGCTGGTGAGCAAGAGCCGCGAT
CTGGGCAATGGCAATGCGCGCAGCGTGACGGTGGTCGAATCGGGGCAGCGTACCCGGGTGGTGGTCAACCTGGTGGCGTT
GGCGCCCTACAGCACCAGGGTTGTCGGTAATCAACTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCAGAGTGCGC
CGGGGCTGCCTGATGTCGCGCCTGGGCGGGCCAAGGCCGCGGCTTCCGCGGGGCGGTCGATTCGCAGCGTGGATTTCCAG
CGCGGCGAGCAGGGCGAGGGCAATGTACTGATCGACCTCTCCGATTCGGGAATGAGCCCGGATATACAGGAGCGCGACGG
CAAGCTCGTTCTCACCTTCGCCAAGGCTCGCCTGCCAGAGCCCTTGCGGGTCAGGCTCGACGTCAAGGATTTCGCCACCC
CGGTGCAGTTCGTCAGTGCCCGGGCGGAGTCCGGCAAGGCCATCATCACCATCGAGCCCGGCGGCACCTTCGATTACTCG
ACCTACCAGACGGACAACAAGCTCACGGTGAGCGTCAGGCCGATGACTTCGGGCGATCTGCAGAAACGCAACGCCGAGCG
CTCGGCCTATAACGGCGAGAAGCTCTCGCTGAACTTCCAGGACATCGAGGTTCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAATGTGTTGCTGGTGGCGCCGGCCGATGA
AATCGCCGCCCGCGAACGCCAGGAACTGGAGTCACAGAAGCAGATCGCCGACCTGGAGCCGCTGCGGCGCGAACTGCTGC
AGGTCAACTACGCCAAGGCCGCAGAAATCGCCAAGTTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGTCAGACGAGCGT
GGTTCGATTACCGTCGATGAACGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCAGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCTAACGTCGACTACGACAAAA
GTCTGGGCGTGCGCTGGGGAGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGC
TCTTCCACCATAGGTACGCCGGGCAGTACCAGCAGCAATTCACCCTTCGTCGACCTGGGCGCCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAACTGACGGCCATGGAAAAGACCGGTAACGGTGAAA
TCGTCTCCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGTACGGAAATCCCCTATCAGGAA
GCCGCTTCCAGTGGGGCGACCTCGGTGTCCTTCAAGGAGGCCTCGCTGTCCCTGGAGGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCA
AGAAAAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTTATCGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGACTTTTCCGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(298-344)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

AMIN domain (AMIN)

(40-122)

Bacterial type II and III secretion system protein (Secretin)

(525-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.769

100

0.733
  comQ Acinetobacter baylyi ADP1

40.863

100

0.412
  pilQ Acinetobacter baumannii strain A118

37.633

100

0.41
  pilQ Acinetobacter baumannii D1279779

37.633

100

0.41
  pilQ Legionella pneumophila strain Lp02

38.279

97.681

0.374
  pilQ Legionella pneumophila strain ERS1305867

38.358

97.101

0.372

Multiple sequence alignment