Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C4K38_RS02310 Genome accession   NZ_CP027707
Coordinates   510026..511078 (+) Length   350 a.a.
NCBI ID   WP_053277119.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. piscium strain DSM 21509     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 505026..516078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K38_RS02300 (C4K38_0460) - 505983..507251 (+) 1269 WP_053277117.1 malic enzyme-like NAD(P)-binding protein -
  C4K38_RS02305 (C4K38_0461) - 507374..509809 (-) 2436 WP_164487031.1 penicillin-binding protein 1A -
  C4K38_RS02310 (C4K38_0463) pilM 510026..511078 (+) 1053 WP_053277119.1 pilus assembly protein PilM Machinery gene
  C4K38_RS02315 (C4K38_0464) - 511078..511644 (+) 567 WP_053277120.1 PilN domain-containing protein -
  C4K38_RS02320 (C4K38_0465) pilO 511641..512258 (+) 618 WP_053277121.1 type 4a pilus biogenesis protein PilO -
  C4K38_RS02325 (C4K38_0466) - 512255..512782 (+) 528 WP_053277122.1 pilus assembly protein PilP -
  C4K38_RS02330 (C4K38_0467) pilQ 512796..514868 (+) 2073 WP_053277123.1 type IV pilus secretin PilQ Machinery gene
  C4K38_RS02335 (C4K38_0468) aroK 514873..515391 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37234.58 Da        Isoelectric Point: 4.7547

>NTDB_id=278958 C4K38_RS02310 WP_053277119.1 510026..511078(+) (pilM) [Pseudomonas chlororaphis subsp. piscium strain DSM 21509]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGNRFRVEAYGVESLPASAVVEKNIVEPEGVGHALSRLLARVRPGAKHAAL
ALAGSAVISKVIEMEAGLSDDDRESQLKVDADQYIPYPLDEASIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEF
AGLTARVVDLEACALERSFALAAGSPVDRTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSIEEAD
LAKKQGGLPDDYVSEVLQPFQEAVVQQVSRSLQFLQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLISNPFVGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=278958 C4K38_RS02310 WP_053277119.1 510026..511078(+) (pilM) [Pseudomonas chlororaphis subsp. piscium strain DSM 21509]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCAACCGCTTTCGGGTCGAGGCCTATGGGGTGGAGTCGTTGCCGGCCAGCGCCGTGGTCGAGAAAAACA
TCGTCGAACCCGAAGGGGTGGGGCATGCCCTGTCTCGCCTGCTGGCCAGGGTCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGCTGGCCGGTTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGACTTTCCGATGACGATCGGGAGAGCCAGCT
CAAGGTTGACGCCGACCAGTACATTCCCTATCCGCTGGACGAGGCCTCCATCGATTTCGACGTCCTCGGCTATTCGGCCC
GCAATCCCGAACGGGTCGATGTGCTGTTGGCGGCCTGTCGCAAGGAGCACATCGAAGTTCGCGAGGCGGCGCTCGAGTTC
GCCGGGCTGACGGCGCGGGTAGTGGACCTGGAAGCCTGCGCGCTGGAGCGCTCATTCGCCCTGGCGGCCGGGTCGCCGGT
TGACCGGACCGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACTCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGCTGTTTGGCGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGATAGAAGAGGCCGAC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGACTACGTCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTACAGCA
GGTTTCGCGCTCGTTGCAGTTTCTCCAGGCCTCCGGTCCGCAGCGCCAGGTCGACTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGGCTTGCGCCATTGATCGAGCAGCGCCTGGGCTTGCCCGTGTTGATCTCCAATCCCTTCGTCGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCGTCCCTGATGATCGCCTGTGGCCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.261

100

0.546
  comM Acinetobacter nosocomialis M2

53.977

100

0.543
  comM Acinetobacter baylyi ADP1

51.42

100

0.517
  pilM Legionella pneumophila strain ERS1305867

45.763

100

0.463

Multiple sequence alignment