Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C6568_RS03995 Genome accession   NZ_CP027667
Coordinates   861152..862888 (+) Length   578 a.a.
NCBI ID   WP_106682998.1    Uniprot ID   A0A2R3Q9R2
Organism   Melaminivora suipulveris strain SC2-9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 856152..867888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6568_RS03965 (C6568_03965) proB 856713..857852 (-) 1140 WP_106682993.1 glutamate 5-kinase -
  C6568_RS03970 (C6568_03970) cgtA 857934..859013 (-) 1080 WP_106682994.1 Obg family GTPase CgtA -
  C6568_RS03975 (C6568_03975) rpmA 859136..859393 (-) 258 WP_106682995.1 50S ribosomal protein L27 -
  C6568_RS03980 (C6568_03980) rplU 859420..859731 (-) 312 WP_106682996.1 50S ribosomal protein L21 -
  C6568_RS03985 (C6568_03985) - 859962..860891 (+) 930 WP_234026798.1 polyprenyl synthetase family protein -
  C6568_RS03995 (C6568_03995) pilB 861152..862888 (+) 1737 WP_106682998.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C6568_RS04000 (C6568_04000) pilC 862921..864141 (+) 1221 WP_106682999.1 type II secretion system F family protein Machinery gene
  C6568_RS04005 (C6568_04005) pilD 864141..865025 (+) 885 WP_106683000.1 prepilin peptidase Machinery gene
  C6568_RS04010 (C6568_04010) coaE 865022..865636 (+) 615 WP_106683001.1 dephospho-CoA kinase -
  C6568_RS04015 (C6568_04015) zapD 865689..866444 (+) 756 WP_106685330.1 cell division protein ZapD -
  C6568_RS04020 (C6568_04020) - 866441..866671 (+) 231 WP_106683002.1 DNA gyrase inhibitor YacG -
  C6568_RS04025 (C6568_04025) - 866659..866949 (-) 291 WP_106683003.1 DUF1330 domain-containing protein -
  C6568_RS04030 (C6568_04030) - 866972..867670 (-) 699 WP_106683004.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63225.49 Da        Isoelectric Point: 5.3333

>NTDB_id=278821 C6568_RS03995 WP_106682998.1 861152..862888(+) (pilB) [Melaminivora suipulveris strain SC2-9]
MVAAVPPTSKQAQPVALPGLARALMSVGKLDQQTAEELFRKSQATRTHFITTLTSAGVITNADLAHTVSSVFGAPLLDLD
ALDPLRISRDLLDIKLCKAYGVVALAKRSNRLIVATADPTNQEAAEKIKFTTQMGVDWVIAEHDKLLRLIEAAAKGDEAL
RASADLEDFSFDDIQPEATEEDADGAASEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPP
IAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIEALGYEPEEKE
RLMTAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNERAGLTFATALRAFLRQDP
DIIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPACKEPADIP
HETLLDAGYNEDELDGSWVTYRPVGCSACNNGYKGRVGIYQVMPITEEMQQIILRDGSALDIAEQARRDGVRSLRESGLH
KARLGVTSLEEVLAVTNE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=278821 C6568_RS03995 WP_106682998.1 861152..862888(+) (pilB) [Melaminivora suipulveris strain SC2-9]
ATGGTCGCCGCCGTTCCACCCACCTCGAAACAGGCCCAGCCCGTGGCCCTGCCGGGTCTTGCCCGCGCCCTGATGTCGGT
GGGAAAGCTCGATCAGCAGACAGCCGAGGAGTTGTTTCGCAAGTCGCAGGCGACACGCACCCACTTCATCACCACGCTCA
CGTCGGCTGGGGTCATCACCAACGCCGATCTGGCGCACACCGTTTCGTCGGTGTTCGGCGCGCCGCTGCTCGATCTGGAC
GCGCTCGATCCACTGCGCATCTCGCGCGACCTGCTTGACATCAAGCTGTGCAAGGCCTACGGCGTGGTGGCGCTGGCCAA
GCGCAGCAATCGGCTGATAGTCGCCACGGCGGACCCCACCAACCAGGAGGCGGCCGAGAAAATCAAGTTCACGACCCAGA
TGGGTGTGGACTGGGTCATCGCCGAGCACGACAAGCTGCTGCGGCTGATCGAAGCCGCCGCCAAAGGCGACGAGGCGCTG
CGCGCGTCGGCGGATCTGGAAGACTTCAGCTTCGACGACATTCAGCCGGAGGCCACCGAAGAAGACGCAGACGGTGCGGC
CAGTGAGGTCGAAGACGCACCCATCGTCAAGTTTCTGCACAAGATGCTGCTGGACGCCTTCAACATGCGCGCGTCCGACC
TGCACTTTGAGCCCTACGAACACCAGTACCGGGTGCGCTTTCGTATCGACGGCGAACTGCGCGAAATCGCCTCGCCGCCA
ATCGCCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTTATCTCGCGCCTGGACATCTCGGAAAAGCGCGTGCCGCAGGA
CGGCCGCATGAAGCTGAAAGTGGGGCCCGACCGGGTGATCGACTTTCGCGTCAGCACGCTACCCACGTTGTTCGGCGAGA
AGATCGTTATTCGTATTCTGGACCCGAGCAGTGCCCGGCTGGGCATCGAGGCGCTCGGATACGAGCCCGAGGAAAAGGAG
CGGCTGATGACTGCCATCGGCCGGCCTTACGGAATGATTCTGGTCACCGGGCCAACTGGCTCGGGCAAGACGGTGTCGCT
CTACACCTGCCTGAACCTGCTGAACAAGCCTGGGGTCAACATCGCCACGGCGGAAGATCCTTCGGAAATCAACCTGCCGG
GCGTGAACCAGGTCAACGTCAACGAAAGAGCCGGCCTGACCTTTGCCACAGCCCTGCGCGCCTTCCTGCGCCAGGACCCA
GACATCATCATGGTCGGTGAAATCCGCGACCTGGAAACCGCCGACATCGCCATCAAGGCCGCCCAAACCGGCCACTTGGT
GCTGTCCACATTGCACACCAACGACGCGCCGACCACGCTCACGCGCATGCGCAACATGGGCATCGCGCCGTTCAACATCG
CCTCCAGCGTCATCCTGATCACCGCCCAGCGCCTGGCGCGGCGGCTGTGCCCCGCCTGCAAGGAGCCTGCCGACATCCCG
CACGAAACGCTGCTGGATGCCGGCTACAACGAAGACGAACTGGACGGCTCATGGGTGACCTACCGGCCCGTGGGCTGCTC
CGCGTGCAACAACGGTTACAAGGGCCGGGTTGGCATCTACCAGGTCATGCCGATCACGGAGGAAATGCAGCAGATCATTC
TGCGCGACGGCAGCGCGCTGGACATCGCCGAGCAGGCGCGGCGCGACGGTGTGCGCTCGCTGCGCGAGTCCGGGCTGCAC
AAGGCCCGCCTGGGAGTGACTTCGCTGGAAGAAGTGCTGGCCGTCACCAATGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R3Q9R2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.674

98.097

0.536
  pilB Acinetobacter baumannii D1279779

57.692

89.965

0.519
  pilF Neisseria gonorrhoeae MS11

51.865

97.405

0.505
  pilB Legionella pneumophila strain ERS1305867

49.383

98.097

0.484
  pilB Vibrio campbellii strain DS40M4

45.633

97.059

0.443
  pilB Vibrio cholerae strain A1552

47.458

91.869

0.436
  pilB Vibrio parahaemolyticus RIMD 2210633

44.681

97.578

0.436
  pilF Thermus thermophilus HB27

40.174

99.481

0.4
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.748

92.561

0.377

Multiple sequence alignment