Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C4901_RS03605 Genome accession   NZ_CP027663
Coordinates   683024..684733 (-) Length   569 a.a.
NCBI ID   WP_205736164.1    Uniprot ID   -
Organism   Acidiferrobacter sp. SPIII_3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 678024..689733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4901_RS03575 (C4901_03565) - 678962..679372 (+) 411 WP_065968819.1 hypothetical protein -
  C4901_RS03580 (C4901_03570) - 679431..679838 (+) 408 WP_110136168.1 roadblock/LC7 domain-containing protein -
  C4901_RS03585 (C4901_03575) - 679963..681033 (+) 1071 WP_110136169.1 malate dehydrogenase -
  C4901_RS03590 (C4901_03580) - 681018..681272 (+) 255 WP_110136170.1 hypothetical protein -
  C4901_RS03595 (C4901_03585) coaE 681289..681888 (-) 600 WP_110136171.1 dephospho-CoA kinase -
  C4901_RS03600 (C4901_03590) - 681881..682780 (-) 900 WP_205736163.1 A24 family peptidase -
  C4901_RS03605 (C4901_03595) pilB 683024..684733 (-) 1710 WP_205736164.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C4901_RS03610 (C4901_03600) - 684913..685332 (+) 420 WP_110136173.1 rubrerythrin family protein -
  C4901_RS03615 (C4901_03605) - 685406..686755 (+) 1350 WP_110136174.1 heterodisulfide reductase-related iron-sulfur binding cluster -
  C4901_RS03620 (C4901_03610) - 686789..687085 (+) 297 WP_110136175.1 encapsulin-associated ferritin-like protein -
  C4901_RS03625 (C4901_03615) - 687108..687689 (+) 582 WP_110136176.1 DUF3501 family protein -
  C4901_RS03630 (C4901_03620) parE 687689..689581 (+) 1893 WP_304529291.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62022.40 Da        Isoelectric Point: 5.8843

>NTDB_id=278747 C4901_RS03605 WP_205736164.1 683024..684733(-) (pilB) [Acidiferrobacter sp. SPIII_3]
MANDGVLLTGLAARLVRDRLLTPAEAERLNAESLTNKVSFVTRLVESKKLDALTIAKVASEEFGIPLLDISAFDSETFPI
KLVDAKLIRRHRVLPIFKRGNRLFVAVADPTNLTALDEIKFNTGLLPEPLLVEEGKLGQLIDRNLSDTGDSLADMAVSES
LEGIDTTGDSAEPADAAAESDINDKPIVRFVNKILLDAINRGASDIHIEPYERTYRVRFRTDGVLQEVAAPPIALAARIA
ARVKILARLDISERRIPQDGRMKIRVSKTRAIDFRVSTLPTLFGEKVVMRLLDPASASLGVDKLGFESDQKAIYLEAIER
PYGMVLVTGPTGSGKTVTLYTALNILNTPDRNISTAEDPVEINLAGINQVNINERAKLTFATALRAFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHMVISTLHTNDAPQTLGRLVNMGVPPFNIASAVNLIIAQRLARRLCVHCRKPLELPEAVLRRAG
FRAEDIPDLKIFGPGGCDQCNGGYKGRVGIYQVMPVSPAIGQIIMNGGNANDIAEQAQKDGVSDLRQSGLKKVREGITSL
EEVERVTNE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=278747 C4901_RS03605 WP_205736164.1 683024..684733(-) (pilB) [Acidiferrobacter sp. SPIII_3]
ATGGCAAATGACGGCGTGTTATTGACAGGGCTTGCGGCGCGCCTCGTGCGCGACCGCCTGTTGACACCGGCCGAGGCCGA
ACGCCTGAATGCCGAATCACTGACTAACAAGGTCTCCTTCGTGACACGGCTCGTCGAGAGCAAGAAGCTCGACGCCCTGA
CCATCGCCAAGGTCGCCTCCGAGGAGTTTGGCATCCCGCTGCTTGACATCAGCGCCTTCGACAGCGAGACCTTTCCGATC
AAGCTCGTCGACGCCAAACTCATAAGGCGCCACCGCGTACTGCCGATCTTCAAGCGCGGAAATCGCCTGTTCGTGGCCGT
GGCCGACCCCACCAATCTGACGGCCTTAGACGAGATCAAGTTCAACACAGGGCTGCTCCCGGAACCCCTGCTCGTCGAAG
AGGGCAAGCTCGGGCAGCTCATAGACCGCAATCTCAGCGACACCGGCGATTCACTGGCCGACATGGCGGTGAGCGAATCC
CTGGAAGGCATCGACACGACCGGAGACAGCGCCGAACCCGCCGATGCCGCCGCCGAAAGCGACATCAATGACAAGCCCAT
CGTCCGCTTCGTCAACAAGATCCTGCTCGACGCCATCAACCGCGGGGCCTCGGACATCCACATAGAGCCCTACGAGCGGA
CTTACCGGGTGCGATTCCGGACCGACGGCGTCTTGCAGGAGGTTGCCGCCCCGCCGATCGCGCTCGCGGCGCGTATCGCC
GCGCGCGTCAAGATCCTGGCGCGGCTCGACATCTCCGAGCGGCGCATTCCCCAGGACGGGCGCATGAAGATCCGCGTATC
CAAGACGCGCGCGATCGATTTCCGGGTCAGCACCCTGCCGACTCTGTTTGGCGAAAAGGTCGTGATGCGTCTGCTCGATC
CGGCATCGGCCTCGCTTGGTGTCGACAAACTCGGGTTCGAGTCCGATCAGAAGGCCATCTACCTGGAGGCGATCGAGCGG
CCCTATGGCATGGTCCTCGTCACCGGACCGACCGGCAGCGGCAAGACCGTGACCCTGTATACCGCGCTCAACATCCTGAA
CACCCCGGATCGCAACATTTCGACCGCCGAAGACCCGGTCGAGATCAATCTTGCCGGCATCAACCAGGTCAACATCAACG
AACGCGCCAAACTGACCTTCGCCACCGCGCTGCGCGCCTTTCTGCGCCAGGACCCCGACATCATCATGGTCGGCGAGATC
CGTGATCTCGAGACCGCGGAGATCGCGATCAAGGCCGCGCAGACCGGACATATGGTGATCTCGACTTTGCATACCAACGA
CGCGCCCCAGACCCTGGGGCGGCTCGTAAACATGGGCGTGCCCCCCTTCAATATCGCCTCGGCGGTCAATCTCATCATCG
CCCAGCGCCTGGCGCGGCGACTGTGCGTGCACTGTCGCAAGCCCCTGGAACTCCCGGAAGCGGTCCTGCGCCGCGCGGGC
TTTCGCGCGGAAGACATCCCCGACCTGAAGATCTTCGGCCCGGGGGGCTGCGATCAATGCAACGGCGGCTACAAGGGCCG
CGTCGGCATCTATCAGGTCATGCCGGTAAGTCCGGCGATCGGGCAGATCATCATGAACGGCGGCAACGCCAACGACATCG
CCGAGCAGGCGCAAAAGGACGGGGTCTCGGACCTGCGTCAATCGGGCCTGAAAAAGGTGCGTGAAGGCATCACGAGCCTC
GAGGAAGTGGAGCGCGTGACCAATGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.614

100

0.557
  pilB Acinetobacter baumannii D1279779

55.044

99.297

0.547
  pilB Acinetobacter baylyi ADP1

53.206

100

0.54
  pilF Neisseria gonorrhoeae MS11

51.056

99.824

0.51
  pilB Vibrio cholerae strain A1552

48.319

99.297

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

47.695

99.121

0.473
  pilB Vibrio campbellii strain DS40M4

46.643

99.473

0.464
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.395

92.443

0.392
  pilF Thermus thermophilus HB27

41.055

93.322

0.383
  pilB/pilB1 Synechocystis sp. PCC 6803

40.769

91.388

0.373

Multiple sequence alignment