Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   ECDH10B_RS17585 Genome accession   NC_010473
Coordinates   3528203..3529327 (-) Length   374 a.a.
NCBI ID   WP_000228551.1    Uniprot ID   P30852
Organism   Escherichia coli str. K-12 substr. DH10B     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3523203..3534327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECDH10B_RS17555 (ECDH10B_3454) yrdA 3525003..3525557 (+) 555 WP_001286216.1 gamma carbonic anhydrase family protein -
  ECDH10B_RS17560 (ECDH10B_3455) yrdB 3525533..3525790 (-) 258 WP_001070563.1 DUF1488 domain-containing protein -
  ECDH10B_RS17565 (ECDH10B_3456) aroE 3525787..3526605 (-) 819 WP_000451243.1 shikimate dehydrogenase -
  ECDH10B_RS17570 (ECDH10B_3457) tsaC 3526610..3527182 (-) 573 WP_001301412.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  ECDH10B_RS17575 (ECDH10B_3458) yrdD 3527187..3527729 (-) 543 WP_001129722.1 type I DNA topoisomerase -
  ECDH10B_RS17580 (ECDH10B_3459) smg 3527758..3528231 (-) 474 WP_000460680.1 DUF494 family protein Smg -
  ECDH10B_RS17585 (ECDH10B_3460) dprA 3528203..3529327 (-) 1125 WP_000228551.1 DNA-protecting protein DprA Machinery gene
  ECDH10B_RS17590 (ECDH10B_3461) def 3529457..3529966 (+) 510 WP_000114984.1 peptide deformylase -
  ECDH10B_RS17595 (ECDH10B_3462) fmt 3529981..3530928 (+) 948 WP_000004473.1 methionyl-tRNA formyltransferase -
  ECDH10B_RS17600 (ECDH10B_3463) rsmB 3530974..3532263 (+) 1290 WP_000744778.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  ECDH10B_RS17605 trkA 3532285..3533661 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  ECDH10B_RS17610 (ECDH10B_3465) mscL 3533791..3534201 (+) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40954.92 Da        Isoelectric Point: 6.3122

>NTDB_id=27874 ECDH10B_RS17585 WP_000228551.1 3528203..3529327(-) (dprA) [Escherichia coli str. K-12 substr. DH10B]
MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=27874 ECDH10B_RS17585 WP_000228551.1 3528203..3529327(-) (dprA) [Escherichia coli str. K-12 substr. DH10B]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATCGCTCACTG
GGTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCACGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATTCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCT
TGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGTAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P30852

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.238

98.663

0.476
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

49.355

82.888

0.409
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Haemophilus influenzae Rd KW20

45.104

90.107

0.406
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39

Multiple sequence alignment