Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C7A17_RS06180 Genome accession   NZ_CP027657
Coordinates   1324136..1325839 (+) Length   567 a.a.
NCBI ID   WP_106737194.1    Uniprot ID   A0A2R3QKR4
Organism   Ectopseudomonas mendocina strain NEB698     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1319136..1330839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7A17_RS06165 (C7A17_06165) - 1319816..1322122 (-) 2307 WP_106737191.1 DUF1631 domain-containing protein -
  C7A17_RS06170 (C7A17_06170) nadC 1322546..1323394 (+) 849 WP_106737192.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C7A17_RS27320 - 1323795..1323906 (-) 112 Protein_1215 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  C7A17_RS06180 (C7A17_06180) pilB 1324136..1325839 (+) 1704 WP_106737194.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C7A17_RS06185 (C7A17_06185) pilC 1325843..1327072 (+) 1230 WP_106737195.1 type II secretion system F family protein Machinery gene
  C7A17_RS06190 (C7A17_06190) pilD 1327079..1327948 (+) 870 WP_106737196.1 A24 family peptidase Machinery gene
  C7A17_RS06195 (C7A17_06195) coaE 1328126..1328734 (+) 609 WP_106737197.1 dephospho-CoA kinase -
  C7A17_RS06200 (C7A17_06200) yacG 1328731..1328925 (+) 195 WP_106737198.1 DNA gyrase inhibitor YacG -
  C7A17_RS06205 (C7A17_06205) - 1329014..1329229 (-) 216 WP_106737199.1 hypothetical protein -
  C7A17_RS06210 (C7A17_06210) - 1329333..1330022 (-) 690 WP_106737200.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62391.37 Da        Isoelectric Point: 5.3348

>NTDB_id=278672 C7A17_RS06180 WP_106737194.1 1324136..1325839(+) (pilB) [Ectopseudomonas mendocina strain NEB698]
MNDVASLSGLAKQMVVAQLLDEKSAQQAQTQAKRNQVPLVTYLVQSKLVKSRDLAELAAEQFGIAFIDISAIDKEGQPKE
LISEKLIRQHRALPLWRRGNKLFIAVSDPTNHQAITDIQFSTGLNTEAVLVEDDKLGDAIEKFFDGAGSGMEDLADVDLD
GLDVEAVDDDKADNTNSSDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKAYRVRFRTDGILHEVARPPIQLSPRIAAR
LKVMAALDISERRKPQDGRIKMKISKNKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEESQKNLYMAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPKQGMDFTQALRAFLRQDPDIIMVGEIRD
LDTASIAVKAAQTGHMVMSTLHTNSAAETLTRLQNMGVPSFNIATSVNLIIAQRLARKLCNSCKKEVDVPRETLLAEGFP
EEKIGTFKLYGPAGCDNCKNGYKGRVGIYEVVKNTPNLQRIIMEEGNSIDIAAQMRKDGFNDLRTSGLLKAMQGVTSLEE
INRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=278672 C7A17_RS06180 WP_106737194.1 1324136..1325839(+) (pilB) [Ectopseudomonas mendocina strain NEB698]
ATGAATGATGTAGCTTCCCTTTCGGGCTTGGCCAAGCAGATGGTGGTTGCTCAGCTCCTAGACGAGAAAAGCGCCCAGCA
AGCCCAAACGCAAGCAAAACGCAACCAAGTCCCATTGGTAACCTACCTCGTACAGAGCAAGCTTGTTAAAAGCCGCGACC
TAGCTGAACTGGCGGCCGAACAGTTCGGCATAGCATTCATTGACATCTCTGCAATCGACAAGGAAGGACAGCCCAAGGAG
CTGATCAGTGAAAAGCTCATTCGCCAGCATAGAGCCCTCCCGCTTTGGCGACGCGGCAACAAGCTTTTCATAGCCGTTTC
TGACCCGACCAACCATCAAGCCATTACAGACATCCAGTTCAGCACGGGACTCAATACCGAAGCGGTTCTGGTAGAAGACG
ACAAGCTGGGTGATGCCATAGAGAAATTCTTCGATGGCGCAGGCAGCGGGATGGAGGATCTAGCCGATGTCGACTTGGAC
GGCCTAGATGTGGAAGCCGTCGACGATGACAAAGCGGACAACACAAACAGTAGTGATGCCGATGATGCACCAGTAGTGCG
TTTCGTAAATAAAATGCTACTAGATGCCATTCGCCTGGGGTCATCCGACTTACACTTTGAGCCTTACGAAAAAGCCTATC
GTGTACGTTTCCGTACAGACGGTATCCTCCATGAGGTGGCCCGGCCGCCCATCCAACTCTCCCCACGAATTGCTGCACGT
CTAAAGGTCATGGCTGCACTCGATATTTCCGAGCGGCGAAAGCCGCAAGATGGCCGGATAAAGATGAAAATATCCAAGAA
CAAGGCCATCGATTTCAGGGTCAACACCCTGCCTACACTATGGGGCGAGAAGATCGTTATGCGAATTCTTGACCCAACCA
GCGCACAAATGGGTATCGATGCCCTAGGCTATGAGGAAAGTCAAAAGAACCTATATATGGCCGCCCTCAAGCAGCCACAG
GGGATGATTCTGGTGACCGGGCCCACAGGCTCAGGCAAGACTGTATCGCTCTATACCGGCCTAAACATCCTCAATACCCC
AGACGTGAATATCTCTACCGCCGAAGACCCAGTGGAAATAAACCTGGAGGGCATCAACCAAGTCAATGTCAATCCCAAGC
AGGGTATGGATTTTACTCAGGCGCTACGCGCCTTCCTGCGCCAGGATCCAGATATCATCATGGTCGGCGAGATCCGAGAC
CTGGATACTGCCTCTATTGCAGTCAAAGCCGCACAGACAGGGCATATGGTGATGTCTACTCTACACACCAATAGCGCGGC
AGAAACCCTCACTCGCTTGCAGAACATGGGCGTGCCGTCCTTCAACATCGCCACCTCGGTCAATTTAATCATTGCTCAGC
GCTTGGCACGCAAACTGTGCAATAGCTGCAAAAAGGAAGTGGACGTACCGCGGGAAACCTTGCTGGCAGAAGGTTTCCCC
GAAGAAAAAATAGGCACCTTCAAGCTCTACGGCCCAGCCGGTTGCGACAACTGCAAGAATGGCTACAAAGGGCGTGTCGG
GATTTATGAAGTAGTTAAAAACACGCCCAACCTACAACGGATTATCATGGAGGAAGGTAACTCCATCGATATCGCAGCAC
AGATGCGCAAAGATGGCTTTAATGACTTGCGTACCTCCGGCCTGCTCAAAGCCATGCAAGGCGTGACCAGCCTCGAGGAA
ATCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R3QKR4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.511

99.471

0.582
  pilB Acinetobacter baylyi ADP1

57.549

99.295

0.571
  pilB Legionella pneumophila strain ERS1305867

55.221

99.647

0.55
  pilF Neisseria gonorrhoeae MS11

49.912

99.647

0.497
  pilB Vibrio cholerae strain A1552

49.822

99.118

0.494
  pilB Vibrio parahaemolyticus RIMD 2210633

51.667

95.238

0.492
  pilB Vibrio campbellii strain DS40M4

49.117

99.824

0.49
  pilB/pilB1 Synechocystis sp. PCC 6803

36.349

100

0.397
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.113

93.651

0.376

Multiple sequence alignment