Detailed information NTG0278674

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: C7A17_RS06190
• Genome accession: NZ_CP027657
• Coordinates: 1327079..1327948 (+)
• Length: 289 a.a.
• NCBI ID: WP_106737196.1
• Uniprot ID: A0A2R3QKX1
• Organism: Ectopseudomonas mendocina strain NEB698
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1322079..1332948

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C7A17_RS06170 (C7A17_06170)	nadC	1322546..1323394 (+)	849	WP_106737192.1	carboxylating nicotinate-nucleotide diphosphorylase	-
C7A17_RS27320	-	1323795..1323906 (-)	112	Protein_1215	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
C7A17_RS06180 (C7A17_06180)	pilB	1324136..1325839 (+)	1704	WP_106737194.1	type IV-A pilus assembly ATPase PilB	Machinery gene
C7A17_RS06185 (C7A17_06185)	pilC	1325843..1327072 (+)	1230	WP_106737195.1	type II secretion system F family protein	Machinery gene
C7A17_RS06190 (C7A17_06190)	pilD	1327079..1327948 (+)	870	WP_106737196.1	A24 family peptidase	Machinery gene
C7A17_RS06195 (C7A17_06195)	coaE	1328126..1328734 (+)	609	WP_106737197.1	dephospho-CoA kinase	-
C7A17_RS06200 (C7A17_06200)	yacG	1328731..1328925 (+)	195	WP_106737198.1	DNA gyrase inhibitor YacG	-
C7A17_RS06205 (C7A17_06205)	-	1329014..1329229 (-)	216	WP_106737199.1	hypothetical protein	-
C7A17_RS06210 (C7A17_06210)	-	1329333..1330022 (-)	690	WP_106737200.1	energy-coupling factor ABC transporter permease	-
C7A17_RS06215 (C7A17_06215)	-	1330496..1331125 (+)	630	WP_106737201.1	DUF1780 domain-containing protein	-
C7A17_RS06220 (C7A17_06220)	-	1331122..1331616 (+)	495	WP_106737202.1	MOSC domain-containing protein	-
C7A17_RS06225 (C7A17_06225)	-	1331824..1331997 (+)	174	WP_106737203.1	DUF3094 family protein	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31928.89 Da
• Isoelectric Point: 7.1972

>NTDB_id=278674 C7A17_RS06190 WP_106737196.1 1327079..1327948(+) (pilD) [Ectopseudomonas mendocina strain NEB698]
MSFVIFLATHTVFFTFCACIFGLLIGSFLNVVVYRVPLMLQRDWQSQAREVLELPPAPPAQTFNLVLPNSSCPNCGHEIR
PWENIPVISYLFLRGKCSNCKTPISKRYPIVELACGLLSGYVAWHFGFTWQTGAMLVLAWGLLAMSLIDADHQILPDVLA
LPLLWLGLIVNYFGLFTSLEDALWGAIAGYLSLWSVYWLFKLITGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVILLRLRGSDSSTPIPFGPYLAIAGWIALLWGDVITGSYLKFAGF

Nucleotide

• Length: 870 bp

>NTDB_id=278674 C7A17_RS06190 WP_106737196.1 1327079..1327948(+) (pilD) [Ectopseudomonas mendocina strain NEB698]
ATGTCCTTCGTTATTTTCCTAGCCACCCACACCGTGTTCTTTACTTTTTGTGCTTGCATATTTGGCCTGCTGATTGGCAG
CTTTCTCAATGTGGTGGTCTACCGCGTTCCGCTGATGCTGCAGCGTGACTGGCAAAGCCAGGCGCGTGAGGTGTTGGAGC
TACCGCCAGCACCTCCGGCACAAACCTTCAATCTGGTTCTACCCAACTCCTCCTGCCCAAACTGTGGCCATGAGATCAGG
CCTTGGGAAAATATCCCTGTCATCAGCTATTTATTCCTTCGCGGTAAATGCTCGAACTGCAAAACGCCGATCAGCAAACG
CTACCCCATCGTCGAACTGGCCTGCGGGCTGCTTTCCGGTTATGTCGCCTGGCATTTCGGCTTTACCTGGCAGACTGGCG
CGATGCTGGTGTTGGCCTGGGGGTTGCTGGCGATGAGTCTGATCGATGCCGATCACCAGATTCTGCCGGACGTACTGGCT
TTGCCGCTGTTGTGGCTGGGCTTGATCGTCAACTACTTCGGGCTGTTTACCAGCCTGGAAGACGCGCTGTGGGGGGCGAT
TGCTGGCTATCTGAGCCTGTGGTCGGTGTACTGGCTGTTCAAGTTGATCACCGGCAAGGAAGGCATGGGCTATGGGGACT
TCAAGCTGCTGGCCATGCTTGGCGCCTGGGGCGGCTGGCAGATTCTGCCGCTGACCATTCTGTTGTCGTCCCTGGTTGGC
GCAGTGCTTGGGGTAATTTTGCTGCGCCTGCGTGGTAGCGACTCCAGCACGCCTATTCCCTTCGGGCCTTATCTGGCCAT
AGCCGGGTGGATCGCGCTGCTCTGGGGCGATGTGATTACCGGTAGTTATCTGAAATTCGCTGGATTTTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A2R3QKX1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	53.381	97.232	0.519
pilD	Acinetobacter nosocomialis M2	52.669	97.232	0.512
pilD	Vibrio campbellii strain DS40M4	54.044	94.118	0.509
pilD	Vibrio cholerae strain A1552	53.676	94.118	0.505
pilD	Neisseria gonorrhoeae MS11	50.19	91.003	0.457