Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C6Q18_RS02190 Genome accession   NZ_CP027656
Coordinates   500229..502301 (+) Length   690 a.a.
NCBI ID   WP_106696325.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. piscium strain ZJU60     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 495229..507301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6Q18_RS02170 (C6Q18_02170) pilM 497459..498511 (+) 1053 WP_106696323.1 pilus assembly protein PilM Machinery gene
  C6Q18_RS02175 (C6Q18_02175) - 498511..499077 (+) 567 WP_025808191.1 PilN domain-containing protein -
  C6Q18_RS02180 (C6Q18_02180) - 499074..499691 (+) 618 WP_063427904.1 type 4a pilus biogenesis protein PilO -
  C6Q18_RS02185 (C6Q18_02185) - 499688..500215 (+) 528 WP_106696324.1 pilus assembly protein PilP -
  C6Q18_RS02190 (C6Q18_02190) pilQ 500229..502301 (+) 2073 WP_106696325.1 type IV pilus secretin PilQ Machinery gene
  C6Q18_RS02195 (C6Q18_02195) aroK 502306..502824 (+) 519 WP_009041824.1 shikimate kinase AroK -
  C6Q18_RS02200 (C6Q18_02200) aroB 502973..504073 (+) 1101 WP_025808185.1 3-dehydroquinate synthase -
  C6Q18_RS02205 (C6Q18_02205) - 504084..505673 (+) 1590 WP_106696326.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74570.80 Da        Isoelectric Point: 5.2971

>NTDB_id=278641 C6Q18_RS02190 WP_106696325.1 500229..502301(+) (pilQ) [Pseudomonas chlororaphis subsp. piscium strain ZJU60]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLANKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAAQSAPGLPAVASGPAKAAASVGRSIRSVDFQ
RGELGEGNVLIDLSDSGMSPDIQERDGKLVLAFAKARLPEPLRVRLDVKDFATPVQFVSARAEPGKAIITIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNVERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=278641 C6Q18_RS02190 WP_106696325.1 500229..502301(+) (pilQ) [Pseudomonas chlororaphis subsp. piscium strain ZJU60]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGTTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCATTGCCGGGGGATCGCCTGGAGTTGAAGTTGACCTTCGATGGGCCGGTGCCCGAGCCGCGGG
GTTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAGCTGGCGAACAAGAGTCGCGAT
CTGGGCAATGGCAATGCGCGCAGCGTGACGGTGGTCGAATCGGGGCAGCGTACCCGGGTGGTGGTCAACCTGGTCGCGCT
GGCGCCCTACAGCACCCGGGTCGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCAGAGTGCGC
CGGGGCTGCCTGCTGTCGCGTCCGGGCCGGCCAAGGCCGCGGCTTCCGTGGGGCGGTCGATTCGCAGTGTGGATTTCCAG
CGCGGCGAGCTGGGGGAGGGCAATGTGCTGATCGACCTCTCCGATTCGGGAATGAGCCCGGATATACAGGAGCGCGACGG
TAAGCTCGTCCTCGCCTTCGCCAAGGCCCGCCTGCCAGAGCCCTTGCGGGTCAGGCTCGACGTCAAGGATTTCGCCACGC
CGGTGCAGTTCGTCAGTGCCCGGGCGGAGCCCGGCAAGGCCATCATCACCATCGAACCTGGCGGCACCTTCGATTACTCG
ACCTACCAGACGGACAACAAGCTTACGGTGAGCGTCAGGCCGATGACCTCGGGCGATCTGCAGAAGCGCAACGTCGAGCG
CTCGGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAATGTGTTGCTGGTGGCGCCGGCCGATGA
AATCGCCGCTCGCGAACGCCAGGAGCTGGAGTCACAGAAGCAGATAGCCGACCTGGAGCCGCTGCGGCGCGAACTGCTAC
AGGTCAACTACGCCAAGGCCGCGGAAATCGCCAAGTTGTTCCAGTCGGTCACTCGCGCCGAGGACAAGTCCGATGAGCGT
GGTTCGATCACCGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGC
TCTTCCACCATAGGCACGCCGGGCAGTACCAGCAGCAATTCACCCTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAACTGACGGCCATGGAAAAGACCGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGTACGGAAATCCCCTATCAGGAA
GCCGCTTCCAGTGGGGCGACCTCGGTGTCCTTCAAGGAGGCCTCGCTGTCTTTGGAGGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCAATCA
AGAAAAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTCATAGGTGGAGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGACTTTTCCGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-122)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Secretin and TonB N terminus short domain (STN)

(298-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.629

100

0.732
  comQ Acinetobacter baylyi ADP1

39.973

100

0.422
  pilQ Acinetobacter baumannii strain A118

38.032

100

0.414
  pilQ Acinetobacter baumannii D1279779

38.032

100

0.414
  pilQ Legionella pneumophila strain Lp02

37.982

97.681

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.06

97.101

0.37

Multiple sequence alignment