Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C6Q18_RS02170 Genome accession   NZ_CP027656
Coordinates   497459..498511 (+) Length   350 a.a.
NCBI ID   WP_106696323.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. piscium strain ZJU60     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 492459..503511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6Q18_RS02160 (C6Q18_02160) - 493342..494610 (+) 1269 WP_025808196.1 malic enzyme-like NAD(P)-binding protein -
  C6Q18_RS02165 (C6Q18_02165) - 494807..497242 (-) 2436 WP_199796888.1 penicillin-binding protein 1A -
  C6Q18_RS02170 (C6Q18_02170) pilM 497459..498511 (+) 1053 WP_106696323.1 pilus assembly protein PilM Machinery gene
  C6Q18_RS02175 (C6Q18_02175) - 498511..499077 (+) 567 WP_025808191.1 PilN domain-containing protein -
  C6Q18_RS02180 (C6Q18_02180) - 499074..499691 (+) 618 WP_063427904.1 type 4a pilus biogenesis protein PilO -
  C6Q18_RS02185 (C6Q18_02185) - 499688..500215 (+) 528 WP_106696324.1 pilus assembly protein PilP -
  C6Q18_RS02190 (C6Q18_02190) pilQ 500229..502301 (+) 2073 WP_106696325.1 type IV pilus secretin PilQ Machinery gene
  C6Q18_RS02195 (C6Q18_02195) aroK 502306..502824 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37245.73 Da        Isoelectric Point: 5.1262

>NTDB_id=278640 C6Q18_RS02170 WP_106696323.1 497459..498511(+) (pilM) [Pseudomonas chlororaphis subsp. piscium strain ZJU60]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGNRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLSDDDRESQLKVDADQYIPYPLEEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEM
AGLTARVVDLEACALERSFALAAGSSVARTVALIDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYRLSKEEAG
LVKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQLFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLSLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=278640 C6Q18_RS02170 WP_106696323.1 497459..498511(+) (pilM) [Pseudomonas chlororaphis subsp. piscium strain ZJU60]
GTGCTAGGACTCTTCAGCAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCAACCGCTTTCGGGTCGAGGCCTATGGGGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAGAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCCTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGACTTTCCGATGATGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATTCCCTATCCACTGGAGGAGGCGGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GTAATCCCGAGCGGGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTCGAAATG
GCGGGATTGACGGCGCGGGTGGTGGACCTGGAAGCCTGCGCGCTGGAGCGTTCGTTCGCCCTGGCGGCCGGGTCATCGGT
TGCCCGGACCGTGGCGCTGATCGATATCGGCGCCACCCTGACCACTCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGCGGGCATCAGTTGACCGAGCACATCCAGCGTCGTTACAGGCTGTCGAAAGAAGAGGCCGGC
CTGGTGAAAAAGCAGGGCGGCCTGCCGGACGACTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTGGTACAGCA
GGTTTCGCGCTCGTTGCAGCTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGATTCCATCCTGTTGGCGGGGGGTAGCG
CCTCGGTTCCAGGGCTTGCACCGTTGATCGAGCAACGCCTGAGCTTGCCCGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGATGCCGCGGCCCTGGCCAGCGAGGCCCCGTCCCTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.977

100

0.543
  comM Acinetobacter nosocomialis M2

53.693

100

0.54
  comM Acinetobacter baylyi ADP1

51.136

100

0.514
  pilM Legionella pneumophila strain ERS1305867

44.633

100

0.451

Multiple sequence alignment