Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C6Y56_RS24385 Genome accession   NZ_CP027561
Coordinates   5362679..5364379 (+) Length   566 a.a.
NCBI ID   WP_169431956.1    Uniprot ID   A0A7Z3C8M4
Organism   Pseudomonas fluorescens strain G7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5357679..5369379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6Y56_RS24365 (C6Y56_24635) - 5357941..5360892 (+) 2952 WP_169431954.1 DUF748 domain-containing protein -
  C6Y56_RS24370 (C6Y56_24640) - 5360892..5361188 (+) 297 WP_169431955.1 DUF2845 domain-containing protein -
  C6Y56_RS24375 (C6Y56_24645) - 5361354..5361710 (-) 357 WP_065261581.1 BON domain-containing protein -
  C6Y56_RS24380 (C6Y56_24650) - 5362009..5362452 (-) 444 WP_085732829.1 pilin -
  C6Y56_RS24385 (C6Y56_24655) pilB 5362679..5364379 (+) 1701 WP_169431956.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C6Y56_RS24390 (C6Y56_24660) pilC 5364382..5365599 (+) 1218 WP_169431957.1 type II secretion system F family protein Machinery gene
  C6Y56_RS24395 (C6Y56_24665) pilD 5365601..5366473 (+) 873 WP_169431958.1 A24 family peptidase Machinery gene
  C6Y56_RS24400 (C6Y56_24670) coaE 5366470..5367093 (+) 624 WP_169431959.1 dephospho-CoA kinase -
  C6Y56_RS24405 (C6Y56_24675) yacG 5367090..5367290 (+) 201 WP_039771664.1 DNA gyrase inhibitor YacG -
  C6Y56_RS24410 (C6Y56_24680) - 5367297..5367512 (-) 216 WP_007959584.1 hypothetical protein -
  C6Y56_RS24415 (C6Y56_24685) - 5367578..5368267 (-) 690 WP_169431960.1 energy-coupling factor ABC transporter permease -
  C6Y56_RS24420 (C6Y56_24690) - 5368264..5368731 (-) 468 WP_169431961.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62780.82 Da        Isoelectric Point: 5.5573

>NTDB_id=278134 C6Y56_RS24385 WP_169431956.1 5362679..5364379(+) (pilB) [Pseudomonas fluorescens strain G7]
MNDIALSGLAKQLVQAELLTEKVAQQAWQQAQRNRLSLVSYLVQNKLVKSWQVVEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHASGLEDMADVDLEG
LDVESVDDSKQDAIGGMDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVARPPIQLAGRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCRKPIEIPRETLLKEGFPE
ERIGSFTIYEPVGCDQCNGGYKGRTGIYEVVRNTPELQRLIMAEGNSLEIDSQMRLDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=278134 C6Y56_RS24385 WP_169431956.1 5362679..5364379(+) (pilB) [Pseudomonas fluorescens strain G7]
ATGAATGACATCGCTCTGAGCGGCCTGGCCAAACAGCTGGTCCAGGCCGAACTGCTCACGGAAAAAGTCGCCCAGCAAGC
CTGGCAGCAGGCCCAGCGCAATCGCCTGTCGCTGGTCAGCTATCTGGTACAGAACAAACTGGTGAAGAGCTGGCAGGTCG
TCGAAATTGCCTCGGAGCATTTCGGCATGGCATTCCTCGACCTCAACTGCCTGGACAAGGAAACCCAGCCCAAGGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCATCACGCCCTGCCCCTGTGGCGGCGAGGCAACAAGCTGTTCGTGGGCATTTCCGA
CCCGAGCAATCACCAGGCGATCAACGACATTCAATTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTGGAGGACGACA
AGCTCACCGACGCCATCGAAAAGTTCTTCGACACCCACGCTTCCGGTCTGGAAGACATGGCCGATGTCGACCTTGAAGGA
CTGGACGTCGAGTCAGTCGATGACAGCAAACAGGACGCCATCGGTGGGATGGACGCCGACGATGCTCCGGTGGTGCGCTT
CGTCCACAAGATGCTGCTGGACGCGATCAAAAGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGAACTACCGGG
TGCGGGTACGTACCGACGGCATCCTGCGTGAAGTGGCCAGGCCACCGATCCAGCTGGCCGGGCGTATCGCTGCACGTCTG
AAGGTCATGGCCAGCCTCGACATCTCCGAACGTCGCAAGCCCCAGGACGGGCGTTTGAAGATGCGTCTGTCAAAAACCAA
GTCGATCGATTTCCGGGTCAATACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATTCGGATCCTCGACCCCTCCAGCG
CCCAGATCGGCATCGACGCCCTCGGTTATGAGCCGGAGCAGAAAGAGTTGTACCTCGCCGCCCTGAAGCAGCCACAGGGC
ATGATTCTGGTTACCGGCCCTACCGGTTCAGGCAAGACCGTGTCGCTGTACACCGGGCTCAACATCCTCAATACCGTCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGAAGCAGG
GCCTGGATTTCGCCCAGGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGATCTG
GAGACCGCGGAAATCGCCATCAAGGCGGCGCAGACCGGTCACCTGGTCCTCTCCACACTGCACACCAACAGCGCCGCCGA
AACGCTCACGCGTTTGCACAACATGGGCATCCCCGGCTTCAACATCGCTACATCGGTCAGCCTGATCATCGCCCAGCGTC
TGGCACGCAAGCTGTGCAGCCATTGCCGAAAACCCATCGAGATTCCCCGCGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCAGTTTCACGATCTATGAGCCCGTCGGTTGCGATCAATGCAACGGCGGCTACAAGGGCCGCACCGGCAT
CTATGAAGTGGTCAGGAATACTCCCGAACTGCAGCGGCTGATCATGGCCGAAGGCAACTCACTGGAAATCGACAGCCAGA
TGCGCCTGGACGGCTTCAACGACCTGCGCACCTCGGGACTGATCAAGGCCATGCAGGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z3C8M4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.522

99.823

0.574
  pilB Acinetobacter baumannii D1279779

57.092

99.647

0.569
  pilB Legionella pneumophila strain ERS1305867

56.007

100

0.56
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio campbellii strain DS40M4

49.298

100

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

49.733

99.117

0.493
  pilF Neisseria gonorrhoeae MS11

49.556

99.47

0.493
  pilF Thermus thermophilus HB27

36.559

98.587

0.36

Multiple sequence alignment