Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C6Y56_RS02020 Genome accession   NZ_CP027561
Coordinates   454087..456165 (+) Length   692 a.a.
NCBI ID   WP_169428508.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain G7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 449087..461165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6Y56_RS02000 (C6Y56_02020) comM 451302..452366 (+) 1065 WP_169428504.1 pilus assembly protein PilM Machinery gene
  C6Y56_RS02005 (C6Y56_02025) - 452366..452932 (+) 567 WP_169428505.1 PilN domain-containing protein -
  C6Y56_RS02010 (C6Y56_02030) pilO 452929..453552 (+) 624 WP_169428506.1 type 4a pilus biogenesis protein PilO -
  C6Y56_RS02015 (C6Y56_02035) - 453549..454073 (+) 525 WP_169428507.1 pilus assembly protein PilP -
  C6Y56_RS02020 (C6Y56_02040) pilQ 454087..456165 (+) 2079 WP_169428508.1 type IV pilus secretin PilQ Machinery gene
  C6Y56_RS02025 (C6Y56_02045) aroK 456170..456688 (+) 519 WP_011332081.1 shikimate kinase AroK -
  C6Y56_RS02030 (C6Y56_02050) aroB 456831..457931 (+) 1101 WP_169428509.1 3-dehydroquinate synthase -
  C6Y56_RS02035 (C6Y56_02055) - 457942..459537 (+) 1596 WP_169428510.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75108.27 Da        Isoelectric Point: 5.4082

>NTDB_id=278121 C6Y56_RS02020 WP_169428508.1 454087..456165(+) (pilQ) [Pseudomonas fluorescens strain G7]
MNRIFSTLGFSLWIALLSPMVQAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVASQLATKTRD
LGSGNARSATVVEANDRTRLIINLTQLAPYDARVEGNTLFVVVGQGTKATAPRPAAKTPRSTPVAAPARTYAPVARAIRG
VDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNAGVSGDRATITVEPGGT
FDYSTYQTDNKLTVSIRPLTIDDLQKRNADRNSYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
VDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=278121 C6Y56_RS02020 WP_169428508.1 454087..456165(+) (pilQ) [Pseudomonas fluorescens strain G7]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGCTTTCACCGATGGTTCAAGCGGCCAACCTGAA
GACGCTGGATGTTGCCGCGCTGCCGGGTGATCGCGTCGAGCTGAAGCTGTCGTTCGACGGCCCGCCGCCGCAGCCCAAGG
GTTACACCACCGACTCGCCGGCGCGCATCGCCCTCGACCTGCCGGGAGTAGCCAGCCAGCTCGCGACCAAGACCCGCGAC
CTCGGTAGCGGCAATGCGCGCTCTGCCACGGTGGTCGAGGCCAATGACCGCACCCGGTTGATCATCAATCTGACGCAACT
GGCGCCGTATGACGCCCGGGTGGAGGGCAACACGCTGTTCGTGGTGGTCGGGCAAGGCACCAAGGCCACCGCGCCGCGAC
CTGCCGCCAAGACACCGCGCTCCACGCCGGTGGCAGCGCCGGCGCGGACTTACGCACCCGTCGCCAGGGCCATTCGTGGT
GTGGACTTCCAGCGCGGCACGGCGGGTGAGGGCAATGTGGTCATCGACCTGTCCGACCCGACCATCGCGCCGGACATCCA
GGAACACGACGGCAAAATCATCCTCAACTTTGCCCGCACCCAATTGCCAGAGCCATTGCGGGTGCGTCTCGACGTCAAGG
ATTTCGCCACCCCGGTGCAGTTCGTCAATGCCGGCGTCAGTGGGGATCGGGCGACCATTACCGTTGAGCCCGGCGGCACG
TTCGACTATTCCACCTACCAGACCGACAACAAGCTGACCGTGAGCATCCGGCCGCTGACGATCGACGACCTGCAAAAACG
CAATGCCGACCGCAACAGCTACAGCGGCGAGAAACTGTCCCTCAACTTTCAGGACATCGACGTGCGTTCGGTGCTGCAAC
TGATCGCCGACTTCACCAATCTCAATCTGGTGGCCAGCGACACAGTGCAGGGCGGAATCACCTTGCGCCTGCAAAACGTG
CCGTGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAAGGCCTGGACAAGCGCAAGGTCGGCAACGTGTTGCTGGTCGC
GCCGGCGGATGAAATCGCCGCCCGCGAACGGCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGAGCTGGCGCCACTGCGTC
GTGAACTGCTGCAAGTGAACTACGCCAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTCACCAGTGCTGAGGCCAAG
GTCGACGAACGGGGTTCGATCACCGTCGACGAGCGCACCAACAACATCATCGCCTACCAGACCCAGGATCGGCTCGATGA
ACTGCGGCGGATCGTGGCGCAGCTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCG
ATTACGACAAGAGCCTGGGCGTGCGCTGGGGCGGATCGATCCAGAACAAGGGCAACTGGAACACTTCCGGGGTCAGCAAC
GGTTCGTCGACCACCATCGGCACGCCGGGGAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCAACAACAC
GTCCGGGATCGGCATTGCGTTCATCACCGACAACGTCTTGCTGGACCTTGAGCTGACGGCGATGGAAAAGACCGGCAACG
GCGAAATCGTCTCGCAGCCCAAGGTGGTCACCTCGGACAAAGAGACCGCGAAGATCCTCAAGGGCACCGAGATTCCGTAT
CAGGAAGCCAGTTCCAGCGGCGCTACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAGGTCACGCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCAC
CGATCAAGAAAAACGAGGTCAATGCCAAGGTGCTGGTGAACGACGGCGAGACCATCGTGATCGGGGGCGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

AMIN domain (AMIN)

(37-122)

Secretin and TonB N terminus short domain (STN)

(302-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.168

100

0.746
  comQ Acinetobacter baylyi ADP1

41.27

100

0.413
  pilQ Acinetobacter baumannii D1279779

37.917

100

0.41
  pilQ Acinetobacter baumannii strain A118

37.917

100

0.41
  pilQ Legionella pneumophila strain Lp02

38.37

97.543

0.374
  pilQ Legionella pneumophila strain ERS1305867

38.187

97.254

0.371

Multiple sequence alignment