Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C2E19_RS03180 Genome accession   NZ_CP027218
Coordinates   708558..710639 (+) Length   693 a.a.
NCBI ID   WP_129387979.1    Uniprot ID   A0A481QPP8
Organism   Pseudomonas sp. DTU12.3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 703558..715639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2E19_RS03160 (C2E19_03165) comM 705770..706834 (+) 1065 WP_129387968.1 pilus assembly protein PilM Machinery gene
  C2E19_RS03165 (C2E19_03170) - 706834..707400 (+) 567 WP_129387971.1 PilN domain-containing protein -
  C2E19_RS03170 (C2E19_03175) pilO 707397..708020 (+) 624 WP_129387974.1 type 4a pilus biogenesis protein PilO -
  C2E19_RS03175 (C2E19_03180) - 708017..708544 (+) 528 WP_129387977.1 pilus assembly protein PilP -
  C2E19_RS03180 (C2E19_03185) pilQ 708558..710639 (+) 2082 WP_129387979.1 type IV pilus secretin PilQ family protein Machinery gene
  C2E19_RS03185 (C2E19_03190) aroK 710644..711162 (+) 519 WP_129387982.1 shikimate kinase AroK -
  C2E19_RS03190 (C2E19_03195) aroB 711315..712415 (+) 1101 WP_129387985.1 3-dehydroquinate synthase -
  C2E19_RS03195 (C2E19_03200) - 712426..714015 (+) 1590 WP_129387988.1 AAA family ATPase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 74999.38 Da        Isoelectric Point: 5.7396

>NTDB_id=275789 C2E19_RS03180 WP_129387979.1 708558..710639(+) (pilQ) [Pseudomonas sp. DTU12.3]
MNRIFSTLGFSLWIALLSPMVHAANLRTLDVAALPGDRVELKLAFDSPPPVPKGYTTDSPARIALDLPGVASQLASKNVD
LGSGNARTATVVEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGAPTAAPRTAAVAPRATAAAVAPAKTFVAKNRAIR
GVDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILGFARTQLPEKLRVRLDVKDFATPVQFVNAGVSGDRTVITVEPSG
TFEYSTFQTDNKLTVSIRPMTLDDLQKRNADRNAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNTSGVS
NGSSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIP
YQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFS
NTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=275789 C2E19_RS03180 WP_129387979.1 708558..710639(+) (pilQ) [Pseudomonas sp. DTU12.3]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCACGCGGCCAATCTGCG
GACGCTGGATGTCGCGGCATTGCCCGGCGACCGCGTCGAGCTGAAGCTGGCATTCGACAGTCCACCGCCGGTGCCAAAGG
GCTACACCACCGACTCGCCCGCGCGGATCGCCCTCGATTTGCCGGGTGTTGCCAGCCAGTTGGCCAGCAAGAATGTCGAT
CTGGGCAGCGGCAATGCGCGCACGGCGACGGTGGTCGAAGCCAAGGACCGCACGCGGCTGATCGTCAGTCTCACCCAACT
GGCGCCGTACAGCACGAGGGTCGAGGGCAATAATCTGTTCGTCGTGGTCGGGCAGGGCGCTCCGACAGCCGCACCGCGCA
CCGCCGCTGTAGCACCGCGCGCGACGGCGGCGGCAGTGGCGCCAGCGAAGACGTTCGTGGCGAAAAACCGCGCCATTCGC
GGCGTCGACTTTCAGCGTGGTACGGCGGGTGAAGGCAATGTCGTCATTGATCTGTCTGACCCGACCATCGCCCCGGATAT
CCAGGAGCATGACGGCAAGATCATCCTCGGCTTTGCCCGTACGCAGTTGCCGGAAAAACTGCGGGTGCGCCTCGACGTCA
AGGATTTCGCCACGCCGGTGCAGTTCGTCAATGCCGGTGTGAGCGGTGATCGCACGGTGATTACTGTCGAGCCGAGCGGC
ACTTTCGAATATTCCACCTTCCAGACCGACAACAAGCTGACCGTCAGTATCCGGCCGATGACGCTCGACGATCTGCAAAA
ACGCAACGCCGACCGTAACGCCTACGTCGGTGAAAAGCTTTCGCTGAATTTCCAGGACATCGACGTGCGTTCGGTGCTGC
AACTGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGCGGCATTACTCTGCGTCTGCAAAAC
GTGCCATGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAAGGCCTGGATAAACGCAAGATCGGTAACGTGCTGCTGGT
GGCGCCGGCCGACGAGATTGCCGCCCGCGAACGCCAGGAACTGGAATCGCAGAAGCAGATTGCCGAACTGGCGCCGCTGC
GCCGTGAGCTGCTGCAAGTGAATTACGCCAAAGCGGCAGATATCGCCAAACTGTTCCAGTCAGTGACCAGCGCCGAGGCG
AAAATCGACGAGCGTGGTTCGATCACGGTGGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
TGAGCTGCGGCGAATCGTGGCGCAACTGGATATTCCGGTGCGCCAAGTGATGATCGAGGCGCGCATCGTCGAGGCCAACG
TCGATTACGACAAGAGTCTGGGCGTGCGCTGGGGTGGTTCGGTGCAGAACAAGGGCAACTGGAACACCTCCGGAGTCAGC
AACGGCTCATCGACAACCATTGGCACGCCGGGCAGCACCAGCACTAACTCGCCCTTCGTCGACATGGGCACGGTCGGCAA
TACCTCGGGGATCGGCATTGCTTTCATCACCGACAACGTTTTGCTTGATCTTGAACTGACGGCCATGGAGAAGACCGGCA
ACGGCGAAATCGTCTCGCAGCCCAAGGTCGTCACCTCCGACAAGGAAACTGCGAAGATCCTCAAAGGCACCGAGATTCCG
TATCAGGAAGCCAGCTCCAGCGGCGCTACCTCCGTGTCGTTCAAGGAAGCTTCGTTGTCGCTGGAAGTGACGCCGCAAAT
CACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAAGTGCAGGATGTGC
CGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAACGACGGCGAAACCATCGTGATTGGTGGTGTTTTCTCA
AATACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGT
GGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCATT
GA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Bacterial type II and III secretion system protein (Secretin)

(528-684)

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(39-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A481QPP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.626

100

0.75
  comQ Acinetobacter baylyi ADP1

40.576

100

0.407
  pilQ Acinetobacter baumannii strain A118

37.12

100

0.405
  pilQ Acinetobacter baumannii D1279779

37.12

100

0.405
  pilQ Legionella pneumophila strain ERS1305867

37.722

97.547

0.368
  pilQ Legionella pneumophila strain Lp02

37.722

97.547

0.368

Multiple sequence alignment