Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CSB93_RS32745 Genome accession   NZ_CP027169
Coordinates   6567648..6569351 (+) Length   567 a.a.
NCBI ID   WP_033998207.1    Uniprot ID   A6VBR2
Organism   Pseudomonas paraeruginosa strain AR_0356     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6562648..6574351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSB93_RS35710 - 6563368..6563610 (+) 243 WP_023109353.1 hypothetical protein -
  CSB93_RS35715 - 6563902..6564084 (+) 183 WP_123809553.1 hypothetical protein -
  CSB93_RS32730 (CSB93_6488) tfpZ 6564204..6564956 (-) 753 WP_034081328.1 TfpX/TfpZ family type IV pilin accessory protein -
  CSB93_RS32735 (CSB93_6489) - 6564953..6566893 (-) 1941 WP_034081327.1 hypothetical protein -
  CSB93_RS32740 (CSB93_6490) - 6566958..6567425 (-) 468 WP_034081326.1 pilin -
  CSB93_RS32745 (CSB93_6491) pilB 6567648..6569351 (+) 1704 WP_033998207.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CSB93_RS32750 (CSB93_6492) pilC 6569355..6570572 (+) 1218 WP_034081324.1 type II secretion system F family protein Machinery gene
  CSB93_RS32755 (CSB93_6493) pilD 6570575..6571447 (+) 873 WP_058146767.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CSB93_RS32760 (CSB93_6494) coaE 6571444..6572055 (+) 612 WP_003152767.1 dephospho-CoA kinase -
  CSB93_RS32765 (CSB93_6495) yacG 6572052..6572252 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  CSB93_RS32770 (CSB93_6496) - 6572290..6572499 (-) 210 WP_033998211.1 hypothetical protein -
  CSB93_RS32775 (CSB93_6497) - 6572605..6573294 (-) 690 WP_012077298.1 energy-coupling factor ABC transporter permease -
  CSB93_RS32780 (CSB93_6498) - 6573291..6573761 (-) 471 WP_079385052.1 FAD/FMN-containing dehydrogenase -
  CSB93_RS32785 (CSB93_6499) - 6573758..6574183 (-) 426 WP_003152776.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62612.45 Da        Isoelectric Point: 5.5587

>NTDB_id=275388 CSB93_RS32745 WP_033998207.1 6567648..6569351(+) (pilB) [Pseudomonas paraeruginosa strain AR_0356]
MNDRHPLTGLARQLVLSNLLDENAAQQAHLQAQRSKLPLVTYLVQNKLVKSRDLLELTAEQFGIAYVDLGALDKESFPKD
LISEKLSRQHRVVPLWRRGNKLFIGLSDPANHQAVSDIQFSTGLTTEAILVEDDKLTQTIDKLYENATDSLAGLDDVDLD
GLDVSTGDSSPQEDGSGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPRETLLHEGFP
EELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRSSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=275388 CSB93_RS32745 WP_033998207.1 6567648..6569351(+) (pilB) [Pseudomonas paraeruginosa strain AR_0356]
ATGAATGATCGCCACCCCTTGACCGGACTGGCCAGGCAACTGGTTCTTTCCAATCTTCTGGATGAAAATGCCGCCCAGCA
GGCCCATTTGCAAGCCCAGCGCAGCAAGCTGCCCCTGGTCACCTATCTGGTACAGAACAAGCTGGTCAAGAGTCGCGATT
TGCTGGAACTGACAGCTGAACAGTTCGGCATCGCTTACGTAGATCTTGGTGCTCTGGACAAGGAGAGTTTCCCCAAGGAT
CTGATCAGCGAAAAACTCTCTCGACAGCACCGAGTCGTTCCACTGTGGCGACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCCGCCAACCACCAGGCGGTGTCTGACATTCAGTTCAGCACCGGGCTGACCACGGAAGCGATCCTGGTCGAAGACG
ACAAGCTGACGCAGACCATCGACAAGCTTTATGAAAATGCCACCGACAGCCTGGCTGGCCTGGACGATGTCGATCTCGAC
GGCCTGGATGTATCCACCGGCGACAGTTCGCCCCAGGAGGACGGTTCGGGCGGAGACGCCGACGACGCACCGGTAGTGCG
CTTCGTCAACAAGATGTTGCTGGACGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAACCCTACGAGAAGATCTACC
GGGTGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGCTGGCTAGCCGTATATCTGCGCGT
CTCAAGGTGATGGCCGGCCTCGACATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGATTTCCGGGTCAACACCCTGCCGACCCTCTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCA
GCGCGCAAATGGGCATCGATGCGCTGGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGCCCCACCGGTTCGGGCAAGACGGTCTCGCTGTACACCGGCCTGAACATCCTCAATACAAC
CGATATCAACATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTCGGCGAGATCCGCGAC
CTGGAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTCACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGC
GCCTTGCGCGAAAACTCTGTTCGCATTGCAAGAAAGAACACGATGTTCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCT
GAAGAACTGATCGGCACCTTCAAGCTGTATTCGCCGGTGGGCTGTGAAAATTGCAAAGGCGGTTACAAAGGTCGCGTCGG
TATTTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCTCCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A6VBR2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.511

99.471

0.582
  pilB Acinetobacter baylyi ADP1

57.549

99.295

0.571
  pilB Legionella pneumophila strain ERS1305867

55.342

100

0.557
  pilB Vibrio cholerae strain A1552

50.261

100

0.51
  pilF Neisseria gonorrhoeae MS11

49.647

99.824

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

51.201

95.414

0.489
  pilB Vibrio campbellii strain DS40M4

48.85

99.647

0.487
  pilB/pilB1 Synechocystis sp. PCC 6803

38.745

95.591

0.37
  pilF Thermus thermophilus HB27

39.434

93.474

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.899

92.945

0.362

Multiple sequence alignment