Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   C5Y82_RS10300 Genome accession   NZ_CP027034
Coordinates   2033304..2033921 (+) Length   205 a.a.
NCBI ID   WP_012010348.1    Uniprot ID   A8FEG6
Organism   Bacillus pumilus strain 150a     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2028304..2038921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5Y82_RS10260 (C5Y82_10260) - 2028474..2029592 (+) 1119 WP_238389159.1 NADH-dependent flavin oxidoreductase -
  C5Y82_RS10265 (C5Y82_10265) - 2029641..2030147 (-) 507 WP_012010341.1 PTS glucose transporter subunit IIA -
  C5Y82_RS10270 (C5Y82_10270) - 2030316..2030735 (+) 420 WP_012010342.1 Hsp20/alpha crystallin family protein -
  C5Y82_RS10275 (C5Y82_10275) - 2030756..2031208 (-) 453 WP_012010343.1 YppG family protein -
  C5Y82_RS10280 (C5Y82_10280) - 2031315..2031686 (-) 372 WP_012010344.1 YppE family protein -
  C5Y82_RS10285 (C5Y82_10285) - 2031744..2031977 (-) 234 WP_225970028.1 YppD family protein -
  C5Y82_RS10290 (C5Y82_10290) sspM 2032174..2032278 (+) 105 WP_012010346.1 acid-soluble spore protein SspM -
  C5Y82_RS10295 (C5Y82_10295) - 2032301..2033257 (-) 957 WP_012010347.1 DUF2515 domain-containing protein -
  C5Y82_RS10300 (C5Y82_10300) recU 2033304..2033921 (+) 618 WP_012010348.1 Holliday junction resolvase RecU Machinery gene
  C5Y82_RS10305 (C5Y82_10305) - 2033959..2036670 (+) 2712 WP_012010349.1 PBP1A family penicillin-binding protein -
  C5Y82_RS10310 (C5Y82_10310) - 2036705..2037214 (-) 510 WP_119125039.1 YpoC family protein -
  C5Y82_RS10315 (C5Y82_10315) nth 2037211..2037873 (-) 663 WP_012010351.1 endonuclease III -
  C5Y82_RS10320 (C5Y82_10320) - 2037891..2038595 (-) 705 WP_012010352.1 DnaD domain-containing protein -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 23635.81 Da        Isoelectric Point: 8.9761

>NTDB_id=274649 C5Y82_RS10300 WP_012010348.1 2033304..2033921(+) (recU) [Bacillus pumilus strain 150a]
MIRYPNGKSYQPIQPIGTKKRISGESSYSNRGMTLEADLNETNQYYLVNGIAVIHKKPTPVQIVNVDYPKRSAAVIKEAY
FKQSSTTDYNGVYRGRYIDFEAKETKSTTSFPLKNFHEHQIEHMKQVEKQGGICFVIISAFGSVYYLPASDLFFFWERQK
LNGRKSISKDELMEAGHLMTLGYSPRIDYIKVVETLHFSDESEYQ

Nucleotide


Download         Length: 618 bp        

>NTDB_id=274649 C5Y82_RS10300 WP_012010348.1 2033304..2033921(+) (recU) [Bacillus pumilus strain 150a]
ATGATTCGGTATCCAAACGGAAAATCGTATCAGCCCATTCAACCAATTGGGACAAAAAAACGAATTAGCGGCGAATCCTC
TTATAGTAACCGCGGGATGACTCTTGAGGCTGACTTAAACGAAACCAACCAGTACTATTTGGTCAACGGGATCGCAGTCA
TTCATAAAAAGCCTACCCCCGTTCAAATTGTGAACGTGGATTATCCAAAAAGAAGCGCTGCTGTGATCAAAGAAGCCTAT
TTTAAACAATCATCCACAACAGACTATAATGGAGTGTACAGAGGGCGCTATATCGATTTTGAAGCAAAGGAAACCAAAAG
CACCACCTCGTTTCCGCTCAAAAATTTTCATGAACATCAAATTGAGCATATGAAACAGGTTGAAAAGCAAGGCGGTATTT
GTTTCGTTATTATATCCGCATTTGGCTCCGTTTATTATTTGCCCGCTTCAGATCTCTTTTTCTTCTGGGAACGGCAGAAA
CTAAACGGCCGGAAATCCATTAGTAAAGATGAACTAATGGAAGCCGGTCACTTAATGACACTTGGATATTCGCCAAGAAT
TGATTATATTAAAGTAGTGGAGACACTTCATTTTTCGGATGAAAGTGAGTATCAATAA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8FEG6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

71.782

98.537

0.707

Multiple sequence alignment