Detailed information NTG0027463

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: LLMG_RS06455
• Genome accession: NC_009004
• Coordinates: 1243244..1244092 (-)
• Length: 282 a.a.
• NCBI ID: WP_011835152.1
• Uniprot ID: Q25AZ2
• Organism: Lactococcus cremoris subsp. cremoris MG1363
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1238244..1249092

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLMG_RS06440 (llmg_1270)	xerS	1238314..1239384 (+)	1071	WP_011835149.1	tyrosine recombinase XerS	-
LLMG_RS06445 (llmg_1271)	trmFO	1239434..1240780 (-)	1347	WP_011835150.1	methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO	-
LLMG_RS06450 (llmg_1272)	topA	1240852..1242978 (-)	2127	WP_011835151.1	type I DNA topoisomerase	-
LLMG_RS06455 (llmg_1273)	dprA	1243244..1244092 (-)	849	WP_011835152.1	DNA-processing protein DprA	Machinery gene
LLMG_RS06460 (llmg_1274)	-	1244240..1244620 (-)	381	WP_011835153.1	RidA family protein	-
LLMG_RS06465 (llmg_1275)	budA	1244660..1245370 (-)	711	WP_011835154.1	acetolactate decarboxylase	-
LLMG_RS06470 (llmg_1276)	ilvA	1245506..1246756 (-)	1251	WP_011835155.1	threonine ammonia-lyase IlvA	-
LLMG_RS06475 (llmg_1277)	ilvC	1246815..1247837 (-)	1023	WP_011835156.1	ketol-acid reductoisomerase	-
LLMG_RS06480 (llmg_1278)	ilvN	1247882..1248358 (-)	477	WP_011835157.1	acetolactate synthase small subunit	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31919.06 Da
• Isoelectric Point: 9.9168

>NTDB_id=27463 LLMG_RS06455 WP_011835152.1 1243244..1244092(-) (dprA) [Lactococcus cremoris subsp. cremoris MG1363]
MITNFDLFRWKKAGMINLGVNKLLKFFHKYDRKISLRQMGQVAQVKSIPNFIEQYKNQDVKKLRIDYKKFSSFSILDDLY
PERLREIYNPPVLIFYQGNIDLLKNPKLAFVGSRLAGQSGIKAVQKIVTELNQSFTIVSGLAKGIDTASHLSAIKTKTPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLVLSEYSLGEKPLKYHFPERNRIIAGLSRGVVVVEAKLRSGSLITCERALEEG
RDIFAIPGNIADGTSDGCNHLIQQGAKLVYQAQDILEEYLYN

Nucleotide

• Length: 849 bp

>NTDB_id=27463 LLMG_RS06455 WP_011835152.1 1243244..1244092(-) (dprA) [Lactococcus cremoris subsp. cremoris MG1363]
ATGATTACAAATTTTGATTTGTTTCGTTGGAAAAAAGCAGGAATGATCAATCTTGGTGTAAATAAGCTCTTAAAATTTTT
CCACAAATATGATAGAAAAATAAGCTTACGACAAATGGGACAAGTGGCTCAAGTCAAATCAATCCCTAACTTTATCGAAC
AATATAAAAATCAGGACGTAAAGAAACTCAGGATAGACTATAAAAAATTTTCCTCCTTTTCCATTCTTGATGACCTTTAT
CCCGAGCGTCTAAGAGAAATCTATAACCCACCCGTCTTAATTTTTTATCAGGGAAATATTGATTTATTAAAAAACCCTAA
ACTTGCCTTTGTTGGAAGCCGCCTAGCAGGTCAATCAGGGATAAAAGCTGTCCAAAAAATTGTGACAGAATTGAATCAAT
CCTTTACAATAGTAAGCGGACTTGCCAAAGGGATTGACACAGCCAGCCATCTGTCAGCAATTAAAACTAAAACACCAACA
ATTGCTGTTATTGGTACAGGATTGGATATTTTCTATCCACTAGAAAATCGTAAAATACAGGAATATCTAGCAAAAAATCA
ATTAGTTTTAAGTGAATATTCATTAGGCGAAAAACCACTTAAATACCACTTCCCAGAGCGCAATCGAATTATTGCTGGTC
TTTCACGGGGAGTTGTTGTCGTGGAAGCAAAATTAAGAAGTGGAAGTTTAATTACCTGTGAAAGGGCACTGGAAGAAGGA
AGAGATATCTTTGCCATCCCTGGAAATATTGCAGATGGAACATCTGATGGCTGTAATCATCTTATTCAACAAGGAGCAAA
ACTTGTTTATCAAGCTCAAGATATTTTAGAAGAATATTTGTACAATTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (69-270)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (2-63)

3D structure

• AlphaFold DB: Q25AZ2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Lactococcus lactis subsp. cremoris KW2	98.227	100	0.982
dprA	Streptococcus mutans UA159	58.633	98.582	0.578
dprA/cilB/dalA	Streptococcus mitis SK321	55.357	99.291	0.55
dprA/cilB/dalA	Streptococcus pneumoniae R6	55	99.291	0.546
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	55	99.291	0.546
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	55	99.291	0.546
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	55	99.291	0.546
dprA/cilB/dalA	Streptococcus pneumoniae D39	55	99.291	0.546
dprA	Legionella pneumophila strain ERS1305867	39.13	97.872	0.383
dprA	Haemophilus influenzae Rd KW20	39.925	95.035	0.379