Detailed information NTG0274097

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LL267_RS09295
• Genome accession: NZ_CP032058
• Coordinates: 1843149..1843796 (-)
• Length: 215 a.a.
• NCBI ID: WP_004255240.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain 267
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1838149..1848796

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL267_RS09265 (LL267_1787)	atpF	1838220..1838726 (-)	507	WP_058221761.1	F0F1 ATP synthase subunit B	-
LL267_RS09270 (LL267_1788)	atpB	1838741..1839454 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LL267_RS09275 (LL267_1789)	-	1839499..1839714 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LL267_RS09280 (LL267_1790)	-	1839898..1840674 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
LL267_RS09285 (LL267_1791)	comEC	1840959..1842518 (-)	1560	WP_405023164.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LL267_RS13700 (LL267_02940)	comEC	1842536..1843168 (-)	633	WP_284198769.1	ComEC/Rec2 family competence protein	Machinery gene
LL267_RS09295 (LL267_1792)	comEA	1843149..1843796 (-)	648	WP_004255240.1	ComEA family DNA-binding protein	Machinery gene
LL267_RS09300 (LL267_1793)	-	1843856..1845217 (-)	1362	WP_058221762.1	ABC transporter permease	-
LL267_RS09305 (LL267_1794)	-	1845214..1846146 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LL267_RS09310 (LL267_1795)	-	1846251..1846649 (-)	399	WP_004255236.1	hypothetical protein	-
LL267_RS09315 (LL267_1796)	-	1846753..1847316 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LL267_RS09320 (LL267_1797)	-	1847489..1848667 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23651.83 Da
• Isoelectric Point: 5.0062

>NTDB_id=274097 LL267_RS09295 WP_004255240.1 1843149..1843796(-) (comEA) [Lactococcus lactis subsp. lactis strain 267]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=274097 LL267_RS09295 WP_004255240.1 1843149..1843796(-) (comEA) [Lactococcus lactis subsp. lactis strain 267]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterProScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367