Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C4M74_RS09465 Genome accession   NZ_CP026744
Coordinates   1958342..1959730 (-) Length   462 a.a.
NCBI ID   WP_104227616.1    Uniprot ID   -
Organism   Pasteurella multocida strain 161215033201-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1953342..1964730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4M74_RS09435 - 1953469..1954842 (-) 1374 WP_104884547.1 sodium:alanine symporter family protein -
  C4M74_RS09440 - 1955291..1955560 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  C4M74_RS09445 yacG 1955560..1955763 (-) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  C4M74_RS09450 coaE 1955774..1956394 (-) 621 WP_104227613.1 dephospho-CoA kinase -
  C4M74_RS09455 - 1956406..1957131 (-) 726 WP_104884548.1 A24 family peptidase -
  C4M74_RS09460 pilC 1957131..1958342 (-) 1212 WP_104227615.1 type II secretion system F family protein Machinery gene
  C4M74_RS09465 pilB 1958342..1959730 (-) 1389 WP_104227616.1 GspE/PulE family protein Machinery gene
  C4M74_RS09470 pilA 1959730..1960164 (-) 435 WP_104227617.1 prepilin peptidase-dependent pilin Machinery gene
  C4M74_RS09475 ampD 1960324..1960890 (+) 567 WP_104227618.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C4M74_RS09480 rppH 1961476..1962072 (+) 597 WP_083004799.1 RNA pyrophosphohydrolase -
  C4M74_RS09485 - 1962075..1962869 (+) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  C4M74_RS09490 lgt 1962879..1963682 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  C4M74_RS09495 - 1963688..1964539 (+) 852 WP_083004791.1 thymidylate synthase -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 52101.76 Da        Isoelectric Point: 6.7666

>NTDB_id=272126 C4M74_RS09465 WP_104227616.1 1958342..1959730(-) (pilB) [Pasteurella multocida strain 161215033201-1]
MFHKNPLYTVSATTGEVFEIPQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
KNELKRALQQLSPPKLQVEESIPTAYQVVQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRLQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSVEKHILTAEDPIEIALSGIIQTQVNTQIGLDFKRLLRT
FLRQDPDVIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCCDCS
VETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=272126 C4M74_RS09465 WP_104227616.1 1958342..1959730(-) (pilB) [Pasteurella multocida strain 161215033201-1]
ATGTTTCATAAGAATCCCTTATATACTGTTAGCGCGACGACAGGAGAGGTATTTGAAATCCCGCAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCTGCATGTGATGCCTTTTCTTTTTTAACCAATAAACACATTGAGCCTGTTTTATTATCA
AAAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTAGAAGAAAGCATACCCACGGCTTATCA
AGTCGTGCAATTAGATTCTGATTCCGCAACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGATATTCATTTTGAACCGCAACAGCATAGTTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACGTTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCCGAGTTGGGGATG
ACCGCAGACCAACAACACAGGCTCCAACACGCGTTAAGTCAGCCTCAAGGACTGATTTTAGTCACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTACAGTGCGTTAGCTTGGCTCAATAGCGTAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGAGCGGGATAATCCAAACGCAAGTCAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACGTTATTATGTTAGGGGAAATTCGTGATGAGGAAAGTGCGTTAATCGCATTACGTGCAGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTTGTGATTGCAGT
GTAGAGACACAATCGGCGTGTCGTTGCTATCAAGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAA
AACAGGGTGTTTTGTCACGGATTTTAGTAGTTTACGTGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACTGATGACA
ATGAAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.77

98.052

0.664
  pilB Haemophilus influenzae Rd KW20

67.108

98.052

0.658
  pilB Glaesserella parasuis strain SC1401

58.351

99.784

0.582
  pilB Vibrio parahaemolyticus RIMD 2210633

40.541

100

0.422
  pilB Vibrio campbellii strain DS40M4

39.834

100

0.416
  pilB Acinetobacter baumannii D1279779

38.776

100

0.411
  pilB Vibrio cholerae strain A1552

39.095

100

0.411
  pilB Acinetobacter baylyi ADP1

37.475

100

0.405
  pilB Legionella pneumophila strain ERS1305867

36.401

100

0.385

Multiple sequence alignment