Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   C4M74_RS09470 Genome accession   NZ_CP026744
Coordinates   1959730..1960164 (-) Length   144 a.a.
NCBI ID   WP_104227617.1    Uniprot ID   -
Organism   Pasteurella multocida strain 161215033201-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1954730..1965164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4M74_RS09440 - 1955291..1955560 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  C4M74_RS09445 yacG 1955560..1955763 (-) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  C4M74_RS09450 coaE 1955774..1956394 (-) 621 WP_104227613.1 dephospho-CoA kinase -
  C4M74_RS09455 - 1956406..1957131 (-) 726 WP_104884548.1 A24 family peptidase -
  C4M74_RS09460 pilC 1957131..1958342 (-) 1212 WP_104227615.1 type II secretion system F family protein Machinery gene
  C4M74_RS09465 pilB 1958342..1959730 (-) 1389 WP_104227616.1 GspE/PulE family protein Machinery gene
  C4M74_RS09470 pilA 1959730..1960164 (-) 435 WP_104227617.1 prepilin peptidase-dependent pilin Machinery gene
  C4M74_RS09475 ampD 1960324..1960890 (+) 567 WP_104227618.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C4M74_RS09480 rppH 1961476..1962072 (+) 597 WP_083004799.1 RNA pyrophosphohydrolase -
  C4M74_RS09485 - 1962075..1962869 (+) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  C4M74_RS09490 lgt 1962879..1963682 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  C4M74_RS09495 - 1963688..1964539 (+) 852 WP_083004791.1 thymidylate synthase -
  C4M74_RS09500 tadA 1964566..1965054 (+) 489 WP_083004788.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15086.53 Da        Isoelectric Point: 9.1281

>NTDB_id=272127 C4M74_RS09470 WP_104227617.1 1959730..1960164(-) (pilA) [Pasteurella multocida strain 161215033201-1]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGVSYTLTASGNAVQGVSWTINCGAQSDLFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=272127 C4M74_RS09470 WP_104227617.1 1959730..1960164(-) (pilA) [Pasteurella multocida strain 161215033201-1]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTCATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCGGCAGCAAGTAAAAAGTATTTAAAAGCCATCACGGTTAAAGCAGGGGTAATTACTGTTACTGGACAAGGGAG
TTTGGAAGGAGTTAGCTATACGCTGACAGCGAGTGGTAACGCAGTTCAAGGTGTTTCTTGGACCATTAATTGTGGTGCTC
AATCTGACCTTTTCCCTGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  pilA Vibrio campbellii strain DS40M4

35.333

100

0.368

Multiple sequence alignment