Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MAQU_RS13720 Genome accession   NC_008740
Coordinates   3002242..3003960 (+) Length   572 a.a.
NCBI ID   WP_011786126.1    Uniprot ID   A1U437
Organism   Marinobacter nauticus VT8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2997242..3008960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MAQU_RS13685 (Maqu_2672) - 2997575..2997877 (-) 303 WP_011786117.1 helix-turn-helix domain-containing protein -
  MAQU_RS22505 (Maqu_2674) - 2998195..2998368 (+) 174 WP_011786119.1 hypothetical protein -
  MAQU_RS13690 (Maqu_2675) - 2998643..2998945 (-) 303 WP_011786120.1 CopG family antitoxin -
  MAQU_RS13695 (Maqu_2676) - 2998947..2999219 (-) 273 WP_011786121.1 DUF4258 domain-containing protein -
  MAQU_RS13700 (Maqu_2677) - 2999401..2999724 (-) 324 WP_011786122.1 helix-turn-helix domain-containing protein -
  MAQU_RS13705 (Maqu_2678) - 2999711..2999965 (-) 255 WP_049756940.1 type II toxin-antitoxin system RelE/ParE family toxin -
  MAQU_RS13710 (Maqu_2679) - 3000119..3001114 (+) 996 WP_236078926.1 transposase -
  MAQU_RS13715 (Maqu_2680) - 3001341..3001874 (-) 534 WP_328285611.1 pilin -
  MAQU_RS13720 (Maqu_2681) pilB 3002242..3003960 (+) 1719 WP_011786126.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MAQU_RS13725 (Maqu_2682) pilC 3003963..3005183 (+) 1221 WP_011786127.1 type II secretion system F family protein Machinery gene
  MAQU_RS13730 (Maqu_2683) pilD 3005253..3006128 (+) 876 WP_011786128.1 prepilin peptidase Machinery gene
  MAQU_RS13735 (Maqu_2684) coaE 3006129..3006746 (+) 618 WP_011786129.1 dephospho-CoA kinase -
  MAQU_RS13740 (Maqu_2685) - 3006664..3007824 (+) 1161 WP_236078927.1 hypothetical protein -
  MAQU_RS13745 (Maqu_2686) tsaA 3007833..3008582 (+) 750 WP_011786131.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62857.89 Da        Isoelectric Point: 4.8638

>NTDB_id=27159 MAQU_RS13720 WP_011786126.1 3002242..3003960(+) (pilB) [Marinobacter nauticus VT8]
MASSNRNVTLTGLARRFVEDGLLDEETAKDAFIQASQNRIPLITYLTQNQLADSSKLAFSAAMEFGVSVLDLDSFLPEMM
PEKVVDEKLVRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDAKLRDAIDKYLESQDTTMGDLEDA
DLEGVETEGNEGEDDDGAVSATDVDDAPIVKYVNKMLLDAIRGGASDIHFEPYEKIYRVRYRTDGILKEVSRPSIKLAPK
ISARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKKLYMDAL
AQPQGMILVTGPTGSGKTVSLYTGLNILNTPGINISTAEDPAEINLEGINQVNVNNRVGLGFAEALRAFLRQDPDVIMVG
EIRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCSSCKQPADVPKDVLLK
EGFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELASMIMEEASSIKIAKQAQAEGFRNLRQSALLKVIEGV
TSLEEANRVTKD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=27159 MAQU_RS13720 WP_011786126.1 3002242..3003960(+) (pilB) [Marinobacter nauticus VT8]
ATGGCTAGCAGCAACCGAAACGTGACTTTGACGGGCCTGGCCCGTCGTTTTGTGGAAGACGGTCTTCTGGATGAGGAAAC
TGCAAAAGATGCATTCATCCAGGCGTCCCAAAACCGAATTCCACTAATAACTTACCTGACCCAGAATCAACTTGCCGATA
GTTCCAAGCTCGCATTCTCCGCAGCTATGGAATTCGGCGTTTCGGTATTGGACCTGGATTCCTTTCTTCCCGAAATGATG
CCGGAGAAAGTGGTCGACGAAAAGCTGGTTCGCAAGCACAACGCCCTCCCCCTCTATAAACGAGGTAACCGTTTATTTAT
TGCAGTATCCGACCCCACCAACATTCAGGCGCTGGACGAGATAAAGTTCAACACAGGCCTTAGCACAGACGCTATTTTGG
TGGATGACGCCAAGCTTCGGGATGCTATCGACAAATACTTGGAGTCCCAGGACACGACAATGGGCGACCTGGAAGATGCG
GATTTGGAAGGCGTGGAGACCGAGGGTAATGAAGGCGAGGACGACGATGGCGCCGTATCCGCTACAGATGTTGACGATGC
ACCCATCGTTAAATATGTGAATAAGATGCTGCTGGACGCCATTCGGGGCGGGGCTTCAGATATTCACTTCGAGCCCTACG
AGAAAATCTACCGAGTGCGTTACCGCACCGACGGCATATTGAAAGAGGTTTCTCGCCCTTCTATCAAGCTAGCTCCCAAG
ATCTCTGCGCGCGTAAAGATCATGGCCCAATTAGACATTTCTGAGCGAAGGGTTCCTCAGGATGGCCGCATAAAGATGAA
GCTGTCCAAAACCAAGGCCATCGACTTCCGGGTAAACACACTGCCCACCCTTTGGGGCGAGAAGATCGTTTTGCGGATTC
TGGACCCAAGCCAGGCGAAGCTGGGCATTGATGTGCTGGGCTACGAGGAGGATCAGAAGAAGCTGTATATGGACGCTTTG
GCGCAGCCGCAAGGCATGATTCTGGTCACAGGCCCGACAGGTTCCGGTAAAACCGTTTCGCTTTACACCGGCTTGAACAT
TCTGAACACCCCGGGCATCAACATTTCCACGGCGGAAGATCCGGCAGAGATCAACCTGGAAGGCATTAATCAGGTCAACG
TAAACAACCGGGTGGGACTCGGCTTTGCGGAAGCGTTGCGTGCCTTCCTCCGTCAGGATCCAGACGTGATCATGGTAGGT
GAGATCCGGGACCTGGAAACTGCCAATATCGCCATCAAAGCGGCGCAGACGGGGCACCTGGTTCTTTCCACCCTGCACAC
CAACAGCGCCGCGGAAACCCTTACCCGGATGATGAACATGGGCGTGCCGGCGTTCAACATTGCCACCTCGGTGAGCCTGA
TTATTGCCCAGCGTTTGGGCCGGCGACTCTGCAGTAGCTGCAAACAACCCGCCGATGTGCCTAAAGACGTGCTTTTGAAG
GAAGGGTTCACACAAGAACAAATTGATACAGGCTTCACGTTGTACCGCCCCAAGGGCTGTGATAAATGCAATGGTGGATA
TAAAGGCCGCGTTGGTATTTACGAGGTGGTCAAAATTACCGATGAGCTGGCAAGCATGATTATGGAAGAGGCCAGCTCCA
TCAAAATTGCAAAGCAGGCTCAGGCGGAAGGCTTCCGTAATCTCAGGCAATCGGCGCTTCTCAAGGTTATTGAAGGAGTG
ACCAGTCTTGAAGAAGCCAACCGTGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1U437

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.068

100

0.572
  pilB Acinetobacter baylyi ADP1

57.092

98.601

0.563
  pilB Legionella pneumophila strain ERS1305867

53.899

100

0.544
  pilB Vibrio parahaemolyticus RIMD 2210633

51.526

97.378

0.502
  pilB Vibrio campbellii strain DS40M4

50.088

98.776

0.495
  pilB Vibrio cholerae strain A1552

54.335

90.734

0.493
  pilF Neisseria gonorrhoeae MS11

48.233

98.951

0.477
  pilB/pilB1 Synechocystis sp. PCC 6803

37.236

100

0.4
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.038

92.133

0.369

Multiple sequence alignment