Detailed information NTG0027107

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PING_RS02575
• Genome accession: NC_008709
• Coordinates: 627570..628697 (+)
• Length: 375 a.a.
• NCBI ID: WP_011768908.1
• Uniprot ID: A1SS84
• Organism: Psychromonas ingrahamii 37
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 622570..633697

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PING_RS02565 (Ping_0491)	nagE	623506..624963 (+)	1458	WP_011768906.1	N-acetylglucosamine-specific PTS transporter subunit IIBC	-
PING_RS02570 (Ping_0492)	glnS	625231..626892 (+)	1662	WP_011768907.1	glutamine--tRNA ligase	-
PING_RS21255 (Ping_0493)	-	626982..627200 (-)	219	WP_408635116.1	winged helix-turn-helix domain-containing protein	-
PING_RS02575 (Ping_0494)	pilU	627570..628697 (+)	1128	WP_011768908.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PING_RS02605 (Ping_0495)	dnaQ	630552..631271 (-)	720	WP_011768909.1	DNA polymerase III subunit epsilon	-
PING_RS02610 (Ping_0496)	rnhA	631409..631870 (+)	462	WP_041765839.1	ribonuclease HI	-
PING_RS02615 (Ping_0497)	-	631839..632579 (-)	741	WP_011768911.1	class I SAM-dependent methyltransferase	-
PING_RS02620 (Ping_0498)	gloB	632621..633391 (+)	771	WP_011768912.1	hydroxyacylglutathione hydrolase	-

Sequence

Protein

• Length: 375 a.a.
• Molecular weight: 41280.28 Da
• Isoelectric Point: 5.4838

>NTDB_id=27107 PING_RS02575 WP_011768908.1 627570..628697(+) (pilU) [Psychromonas ingrahamii 37]
MEFEEMLNILSSKDGSDLYLSTGAPPSAKFQGTLEPLTDIPFRAGEVAEIAASIMDSEQKVAFEQELEMNLAISISKVGR
FRVNIFKQRNEVSIVARNIKIEIPSLDELGLPEILKSVITAKRGLVLFIGATGSGKSTSLAALIDHRNTNLSGHIITIED
PIEFVHQHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLAISTLHANNANQALDRI
INFFPEDRRIQLLSDLGNNLKSFISQRLIPTIDGKRCAAIEVMMGSLTIRDMIKRGDFGLLKEVMEKSEGLGMKTFDGAL
FDLTIAGKISEEEAIKNSDSATNLTLKFKLYHGNTTEEAGQDDKKSGGLTLSMMD

Nucleotide

• Length: 1128 bp

>NTDB_id=27107 PING_RS02575 WP_011768908.1 627570..628697(+) (pilU) [Psychromonas ingrahamii 37]
ATGGAATTTGAAGAGATGCTCAATATTTTGTCGAGCAAAGATGGCTCGGATCTTTACCTGTCAACGGGGGCTCCCCCTTC
AGCTAAATTTCAAGGTACATTAGAACCTTTAACGGATATTCCTTTTCGGGCAGGTGAAGTTGCTGAAATAGCCGCTTCGA
TTATGGATAGTGAACAAAAAGTGGCATTTGAGCAAGAGCTTGAAATGAACTTAGCGATATCTATTTCCAAGGTTGGACGC
TTTCGAGTTAATATTTTTAAACAGCGTAATGAAGTTTCAATCGTTGCGCGTAATATTAAAATTGAGATCCCCAGCCTCGA
TGAATTAGGGCTTCCGGAAATACTTAAAAGTGTGATCACGGCTAAACGAGGTTTAGTGCTATTTATCGGTGCAACGGGCT
CAGGTAAATCGACTTCATTAGCAGCCCTTATTGATCACCGAAATACTAATCTCTCCGGTCATATTATTACCATCGAAGAT
CCTATTGAGTTTGTACACCAGCATAAAAAAAGTATCGTCAATCAGCGTGAAGTAGGAGTTGATACGCGTAGTTTTCATGC
GGCCTTAAAAAATACTCTGCGTCAGGCACCCGATGTTATTTTGATTGGTGAAATCCGCGATAGGGAAACAATGGAGCACG
CATTAGCCTTTGCAGAAACCGGCCACTTAGCTATTTCAACATTACATGCGAATAATGCTAACCAGGCATTGGATCGTATT
ATTAACTTCTTTCCAGAAGATCGTCGTATTCAGTTGTTAAGTGATTTAGGTAATAACTTGAAATCCTTTATTTCTCAACG
TTTAATTCCAACGATTGATGGCAAACGTTGTGCTGCCATTGAAGTCATGATGGGAAGCCTAACGATACGCGATATGATTA
AGCGCGGTGATTTTGGTTTGCTGAAAGAGGTGATGGAAAAATCAGAAGGATTAGGGATGAAAACCTTTGATGGTGCATTA
TTTGATCTCACCATTGCAGGAAAAATATCTGAAGAGGAAGCGATAAAAAATTCAGATTCAGCCACTAACCTAACGCTGAA
ATTTAAGTTGTACCATGGTAATACTACTGAAGAAGCAGGACAAGACGACAAAAAATCAGGTGGATTGACGTTATCAATGA
TGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (75-244)

3D structure

• AlphaFold DB: A1SS84

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	53.316	100	0.536
pilU	Acinetobacter baylyi ADP1	51.515	96.8	0.499
pilU	Vibrio cholerae strain A1552	48.108	98.667	0.475
pilT	Pseudomonas aeruginosa PAK	42.216	89.067	0.376
pilT	Legionella pneumophila strain Lp02	41.617	89.067	0.371
pilT	Legionella pneumophila strain ERS1305867	41.617	89.067	0.371
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.593	86.4	0.368