Detailed information NTG0027105

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: PING_RS02515
• Genome accession: NC_008709
• Coordinates: 606886..608574 (+)
• Length: 562 a.a.
• NCBI ID: WP_011768896.1
• Uniprot ID: A1SS72
• Organism: Psychromonas ingrahamii 37
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 601886..613574

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PING_RS02495 (Ping_0476)	-	602675..603100 (+)	426	WP_011768892.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
PING_RS21570 (Ping_0477)	-	603758..604204 (+)	447	WP_011768893.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
PING_RS02505 (Ping_0478)	-	605080..605484 (+)	405	WP_011768894.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
PING_RS02510 (Ping_0479)	-	606497..606886 (+)	390	WP_011768895.1	hypothetical protein	-
PING_RS02515 (Ping_0480)	pilB	606886..608574 (+)	1689	WP_011768896.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PING_RS02520 (Ping_0481)	pilC	609052..610284 (+)	1233	WP_011768897.1	type II secretion system F family protein	Machinery gene
PING_RS02525 (Ping_0483)	-	610888..612108 (-)	1221	WP_011768898.1	EAL domain-containing protein	-

Sequence

Protein

• Length: 562 a.a.
• Molecular weight: 61681.57 Da
• Isoelectric Point: 4.8144

>NTDB_id=27105 PING_RS02515 WP_011768896.1 606886..608574(+) (pilB) [Psychromonas ingrahamii 37]
MATNNISGLASSLATRGLLPFDKLQQITSAAQQDKKSLVSYLVDNKFIDSLTLAEFFEIEYGLPLLDASILDLDEIPEKF
LNNKLIEKHHALPVYVQDKTLYIAMSDPTNIAALEDFAFSFAIHTDALLVEEQYLAKAIETVLEDDVASLGEMSDIEDIE
VDDSDSRLDEAQGNGSDDAPIVKYINSILLDAVRKGASDLHFEPYEKTYRIRFRVDGILHQVATPPVNLANRFSARLKVM
SKLDIAERRLPQDGRIKLKITNKRSVDLRVSTLPTMWGEKVVMRILDSSGANLNIDSLGYNDKQKESYLSALAKPQGMIL
ITGPTGSGKTVSLYSGLNILNTVQRNISTAEDPVEINLSGINQVQINTKAGLTFASALRSFLRQDPDILMVGEIRDLETA
EIAIKAAQTGHMVLSTLHTNSAAETLTRLLNMGVPAFNVASSVSLIIAQRLARRLCPECKTEEVFETPELKTIGFTETQI
AAGITLYKPVGCSKCTEGYKGRVGIYEVMQMSETIGHLILNNGNAPEIAKLAEAEGMDTLRQSALQQVLKGITSLTEVTR
VT

Nucleotide

• Length: 1689 bp

>NTDB_id=27105 PING_RS02515 WP_011768896.1 606886..608574(+) (pilB) [Psychromonas ingrahamii 37]
ATGGCAACCAATAATATTAGTGGTCTGGCATCCAGTCTTGCCACACGTGGTTTACTCCCCTTTGATAAACTTCAGCAGAT
TACCAGCGCGGCACAGCAGGATAAAAAATCCCTGGTAAGTTATCTGGTCGACAACAAATTTATCGATAGCCTGACGCTGG
CGGAGTTCTTCGAAATAGAATATGGTCTTCCGTTATTAGATGCATCTATCCTTGATCTGGATGAAATACCGGAAAAATTT
CTGAATAACAAGTTGATAGAAAAACACCATGCACTGCCGGTTTATGTGCAGGACAAAACCCTGTATATCGCCATGTCTGA
CCCCACTAATATTGCTGCGCTTGAAGACTTTGCTTTCAGCTTTGCTATCCATACCGATGCCCTGCTAGTGGAAGAGCAAT
ACCTTGCTAAAGCCATTGAAACCGTATTGGAAGATGATGTTGCTTCTTTGGGTGAAATGTCCGATATTGAGGATATTGAG
GTTGATGATTCTGATTCGCGTCTGGATGAGGCGCAGGGTAACGGCAGTGATGATGCGCCTATTGTTAAATATATTAACAG
CATTTTATTGGATGCGGTGCGCAAAGGGGCATCGGATCTGCACTTTGAACCCTATGAAAAAACCTATCGTATTCGCTTTC
GTGTTGACGGAATTTTACATCAAGTGGCCACGCCACCGGTGAATCTGGCCAACCGTTTTTCAGCCCGCCTTAAGGTTATG
TCAAAACTGGATATCGCCGAGCGTCGTTTGCCGCAGGACGGGCGTATTAAACTTAAAATTACCAATAAACGCAGTGTGGA
TTTGCGTGTCAGTACACTGCCGACCATGTGGGGCGAAAAGGTGGTAATGCGTATTCTGGACTCATCGGGAGCGAACCTGA
ATATCGACAGTTTAGGCTACAATGATAAGCAGAAAGAAAGCTACCTTAGTGCTCTGGCAAAACCGCAGGGAATGATTTTA
ATCACTGGTCCGACTGGCAGTGGTAAAACTGTTTCTTTGTATAGCGGATTAAATATTCTTAATACCGTGCAGCGTAATAT
TTCTACGGCGGAAGATCCGGTAGAGATTAATTTATCCGGTATTAATCAGGTGCAGATCAATACCAAAGCGGGATTAACCT
TTGCCTCTGCATTGCGTTCTTTTCTACGTCAAGATCCCGATATTTTGATGGTTGGTGAAATTCGTGATTTAGAAACCGCT
GAAATTGCGATCAAAGCCGCGCAAACCGGACATATGGTTTTGTCTACCCTGCATACCAACTCCGCTGCGGAAACCTTAAC
GCGTTTACTTAATATGGGTGTGCCCGCATTTAATGTCGCTTCTTCGGTGTCGCTGATTATCGCGCAGCGTTTGGCGCGGC
GTTTATGCCCTGAGTGTAAAACCGAAGAGGTTTTTGAAACGCCGGAATTAAAAACCATTGGGTTTACAGAAACCCAGATA
GCTGCGGGTATCACGCTCTATAAACCCGTTGGTTGTTCAAAATGTACTGAAGGTTATAAAGGCCGAGTGGGTATCTACGA
AGTGATGCAGATGAGCGAGACGATTGGTCACCTTATTTTAAACAATGGTAATGCTCCGGAGATCGCCAAGCTGGCCGAGG
CCGAGGGGATGGATACACTGCGTCAGTCAGCACTGCAGCAGGTGTTAAAGGGGATCACCAGTTTAACGGAAGTGACCCGG
GTAACCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (187-454)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (42-142)

3D structure

• AlphaFold DB: A1SS72

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Legionella pneumophila strain ERS1305867	54.159	100	0.544
pilB	Acinetobacter baylyi ADP1	53.832	99.822	0.537
pilB	Acinetobacter baumannii D1279779	55.433	96.619	0.536
pilB	Vibrio parahaemolyticus RIMD 2210633	51.95	100	0.521
pilB	Vibrio campbellii strain DS40M4	50.177	100	0.504
pilB	Vibrio cholerae strain A1552	54.795	90.925	0.498
pilF	Neisseria gonorrhoeae MS11	51.282	90.214	0.463
pilB/pilB1	Synechocystis sp. PCC 6803	37.198	100	0.411
pilF	Thermus thermophilus HB27	38.156	98.399	0.375